FastQCFastQC Report
Fri 10 Feb 2017
ERR1631247.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631247.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906786
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.128034618972944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10910.1203150467695796No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9690.10686093521514448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA250.005495738729.629
GCTATAT400.001930874323.1250023
TAGTACA502.7014923E-422.24
TCTAGAC2550.021.0392153
TCTAGTT2600.020.63461528
GACATGT2450.020.3877567
CTCTAGT2450.020.38775627
ATACACA3700.020.037
GGTATCA8450.019.9230771
CTAGACA2950.019.4406784
GTATCAA8250.019.0606062
TATTTTC2550.018.86274513
ACATGTA2550.018.8627458
GTTCTAG2850.018.824561
AGGTATC701.2188375E-418.57
CTAAACA500.00703376418.54
TCTAAGC500.00703376418.53
CTTACAC1205.169568E-918.53
CTAGTTT2900.017.86206829
GTTTTTG3200.017.3437532
Produced by FastQC (version 0.11.5)