FastQCFastQC Report
Fri 10 Feb 2017
ERR1631246.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631246.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313418
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA6290.20069045172900088No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC5140.16399823877377814No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC4160.13273009208150138No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC4130.13177290391745208No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC3980.12698696309720567No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC3610.11518164240726442No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC3300.10529069804542178No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT3150.10050475722517532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAT303.5935585E-430.8333341
CCCTACA250.005490320729.62
ATACCAT250.005490320729.66
TACGTCC250.005490320729.630
GGGTATC405.9265636E-527.751
AAGACGG754.6020432E-1027.1333335
AGACGGA754.6020432E-1027.1333336
CAAGACG809.713403E-1025.43754
TAGGACA451.3202139E-424.6666684
CCATCGG551.8969402E-523.54545419
ATACACA1201.0913936E-1121.58333437
GCGAAAG957.0940587E-921.42105318
GGTATCA2250.021.3777771
CGTCGTA705.082409E-621.14285710
ACCGTCG705.082409E-621.1428578
TACCGTC705.082409E-621.1428577
ATACCGT806.92693E-720.81256
CGGACCA1202.3646862E-1020.0416689
AAGCATT1052.2437234E-819.3809522
GAGCGAA1052.2437234E-819.3809516
Produced by FastQC (version 0.11.5)