##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631246.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	313418
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.82073461000964	34.0	31.0	34.0	31.0	34.0
2	33.006713079657196	34.0	33.0	34.0	31.0	34.0
3	33.119616614234026	34.0	33.0	34.0	31.0	34.0
4	36.44213159422879	37.0	37.0	37.0	35.0	37.0
5	36.42592639861144	37.0	37.0	37.0	35.0	37.0
6	36.467589608765294	37.0	37.0	37.0	35.0	37.0
7	36.45832083671008	37.0	37.0	37.0	35.0	37.0
8	36.43515369251288	37.0	37.0	37.0	35.0	37.0
9	38.293930150789045	39.0	39.0	39.0	37.0	39.0
10	38.2529784505038	39.0	39.0	39.0	37.0	39.0
11	38.31387795212783	39.0	39.0	39.0	37.0	39.0
12	38.09018626881672	39.0	39.0	39.0	37.0	39.0
13	38.214917458473984	39.0	39.0	39.0	37.0	39.0
14	39.74765329368447	41.0	40.0	41.0	38.0	41.0
15	39.752321181297816	41.0	40.0	41.0	38.0	41.0
16	39.73948847864513	41.0	40.0	41.0	38.0	41.0
17	39.71991079006311	41.0	40.0	41.0	38.0	41.0
18	39.685825319541316	41.0	40.0	41.0	37.0	41.0
19	39.69814114058541	41.0	40.0	41.0	37.0	41.0
20	39.68202528253004	41.0	40.0	41.0	37.0	41.0
21	39.643999387399575	41.0	40.0	41.0	37.0	41.0
22	39.58967257783535	41.0	40.0	41.0	37.0	41.0
23	39.52125914912354	41.0	39.0	41.0	37.0	41.0
24	39.48707795978533	41.0	39.0	41.0	37.0	41.0
25	39.469124300454986	41.0	39.0	41.0	37.0	41.0
26	39.41135161350018	41.0	39.0	41.0	36.0	41.0
27	39.3414322087436	41.0	39.0	41.0	36.0	41.0
28	39.24146347689029	41.0	39.0	41.0	36.0	41.0
29	39.166273155977	41.0	39.0	41.0	35.0	41.0
30	39.11418935734387	41.0	39.0	41.0	35.0	41.0
31	39.07564019935039	40.0	39.0	41.0	35.0	41.0
32	38.993357114141496	40.0	39.0	41.0	35.0	41.0
33	38.89907088935543	40.0	39.0	41.0	35.0	41.0
34	38.85813195157904	40.0	38.0	41.0	35.0	41.0
35	38.74213350860512	40.0	38.0	41.0	35.0	41.0
36	38.68810980862618	40.0	38.0	41.0	35.0	41.0
37	38.627746332374016	40.0	38.0	41.0	35.0	41.0
38	38.538877792596466	40.0	38.0	41.0	35.0	41.0
39	38.45584490999241	40.0	38.0	41.0	35.0	41.0
40	38.3802238544053	40.0	38.0	41.0	34.0	41.0
41	38.29560842070334	40.0	37.0	41.0	34.0	41.0
42	38.19449425368039	40.0	37.0	41.0	34.0	41.0
43	37.23937361606544	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	12.0
19	17.0
20	25.0
21	60.0
22	109.0
23	142.0
24	242.0
25	344.0
26	518.0
27	725.0
28	956.0
29	1241.0
30	1748.0
31	2269.0
32	3159.0
33	4313.0
34	6491.0
35	9714.0
36	16096.0
37	29135.0
38	68808.0
39	167290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52047425482902	16.598599952778716	12.060889929742387	27.820035862649878
2	21.08972681849798	18.807790235404475	31.58912378995463	28.513359156142915
3	20.946467656611937	19.332329349303485	27.90043966843002	31.820763325654553
4	15.570579864589781	14.83163060194373	31.614010682219913	37.98377885124658
5	17.58578001263488	33.45500258440804	31.558493768705056	17.40072363425202
6	37.320447453560426	32.340516498733315	14.754736486098437	15.58429956160782
7	32.857079044598585	27.325807707279097	18.986146296638992	20.830966951483322
8	29.94403639867525	30.438583616767385	18.483941573234468	21.133438411322896
9	28.294801192018326	13.569418476284067	17.236725395478242	40.899054936219365
10	19.59683234530244	23.44345251389518	29.22231652298209	27.73739861782029
11	39.88315923144172	19.654263635145398	18.554773497374114	21.90780363603877
12	25.26370533919558	22.884773688811748	25.32783694618688	26.523684025805792
13	32.85612185643454	16.345264152027006	22.982406881544772	27.816207109993684
14	25.504597693814652	18.415981213586967	20.929238269659052	35.150182822939335
15	28.672252391375096	24.394578486238824	18.87032652878903	28.062842593597047
16	28.76541870600923	22.85095304034867	20.557849262007927	27.825778991634177
17	27.946065637583033	22.955286550230046	21.41261829250394	27.686029519682982
18	28.000944425655195	20.949658283825435	23.09822664939474	27.951170641124634
19	28.211206759024687	22.169754130266927	21.8015557498293	27.81748336087908
20	28.908358805173922	20.949977346546785	21.64457689092522	28.497086957354078
21	30.374132947054733	21.53641462838765	21.045058037509012	27.044394387048605
22	30.280647569699255	21.27574038504489	20.54987269397418	27.893739351281678
23	28.124421698817553	21.20937533900414	21.61203249334754	29.054170468830765
24	27.598287271311793	22.24601012066952	21.860582353279007	28.295120254739675
25	27.89948248026597	21.11876152614081	21.557153705275383	29.424602288317836
26	27.972228780733715	22.62314225730494	21.769011352251624	27.63561760970972
27	29.063104225028557	21.00964207543919	21.527799934911204	28.399453764621047
28	27.907459048299714	21.65829658794326	22.163691938561282	28.270552425195746
29	26.572819684893656	21.890893311807236	23.38155434595333	28.154732657345782
30	26.278324793087826	23.37517309152633	23.026118474369692	27.32038364101615
31	28.17834329872566	22.45021026233337	21.232666917662673	28.138779521278295
32	26.653223490673795	21.541200569207895	22.670363540064706	29.1352124000536
33	26.345647027292625	21.27542132232354	23.214046417244703	29.16488523313913
34	27.768666764512567	20.609856485587937	23.63010420588479	27.991372544014702
35	27.186058235327902	20.62134274355653	24.487744800872953	27.704854220242613
36	25.6363705977321	21.583316848426062	24.520927323893332	28.259385229948503
37	26.652904427952446	19.63480080914306	25.147247445902916	28.565047317001575
38	25.051209566776638	19.40635190065663	26.683215386480676	28.859223146086055
39	25.38080135793094	18.755783011824466	27.472576559099988	28.390839071144608
40	24.113803291451035	19.214595205125423	28.295439317461025	28.376162185962517
41	22.858610545661065	19.339029666451832	30.05985616652522	27.742503621361887
42	23.48620691855605	19.32626715759784	29.784824100721718	27.402701823124392
43	21.355187002660983	18.84033463298215	30.380514201481727	29.423964162875137
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	6.0
4	9.0
5	9.0
6	16.0
7	23.0
8	23.0
9	23.0
10	36.0
11	49.0
12	49.0
13	75.5
14	102.0
15	192.5
16	283.0
17	265.0
18	247.0
19	247.0
20	288.0
21	329.0
22	366.0
23	403.0
24	521.5
25	640.0
26	640.0
27	869.0
28	1098.0
29	1468.5
30	1839.0
31	2390.5
32	2942.0
33	2942.0
34	3751.0
35	4560.0
36	5780.5
37	7001.0
38	8353.0
39	9705.0
40	9705.0
41	10873.5
42	12042.0
43	13369.0
44	14696.0
45	16172.5
46	17649.0
47	17649.0
48	19369.5
49	21090.0
50	22591.5
51	24093.0
52	26359.5
53	28626.0
54	28626.0
55	28059.0
56	27492.0
57	27084.0
58	26676.0
59	25908.0
60	25140.0
61	25140.0
62	23848.0
63	22556.0
64	20855.0
65	19154.0
66	16927.5
67	14701.0
68	14701.0
69	12433.0
70	10165.0
71	8789.0
72	7413.0
73	5766.0
74	4119.0
75	4119.0
76	3404.5
77	2690.0
78	2355.0
79	2020.0
80	1825.0
81	1630.0
82	1630.0
83	1344.0
84	1058.0
85	797.0
86	536.0
87	439.0
88	342.0
89	342.0
90	274.0
91	206.0
92	124.5
93	43.0
94	28.5
95	14.0
96	14.0
97	11.0
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	313418.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.51572971470155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.65006756874574	58.48355257884633
2	4.769554796584667	6.154226162318295
3	1.43853828143273	2.784250408474959
4	0.7348397569322739	1.8963489256743828
5	0.4266985009756553	1.3764382579306849
6	0.32000018168133454	1.2387027138005022
7	0.21429398172492548	0.9677732823116739
8	0.15900862660843745	0.8206846061260683
9	0.1261018424949057	0.7321997148234483
>10	0.9702080035437177	12.795213440613686
>50	0.12613247045832385	5.565556806465938
>100	0.0635628197585253	6.818864325858905
>500	9.931690587269579E-4	0.3661887767551554
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	629	0.20069045172900088	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	514	0.16399823877377814	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	416	0.13273009208150138	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	413	0.13177290391745208	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	398	0.12698696309720567	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	361	0.11518164240726442	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	330	0.10529069804542178	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	315	0.10050475722517532	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.190627213497629E-4	0.0	0.0	0.0
6	0.0	3.190627213497629E-4	0.0	0.0	0.0
7	0.0	3.190627213497629E-4	0.0	0.0	0.0
8	0.0	3.190627213497629E-4	0.0	0.0	0.0
9	0.0	3.190627213497629E-4	0.0	0.0	0.0
10	0.0	3.190627213497629E-4	0.0	0.0	0.0
11	0.0	3.190627213497629E-4	0.0	0.0	0.0
12	0.0	3.190627213497629E-4	0.0	0.0	0.0
13	0.0	3.190627213497629E-4	0.0	0.0	0.0
14	0.0	3.190627213497629E-4	0.0	0.0	0.0
15	0.0	3.190627213497629E-4	0.0	3.190627213497629E-4	0.0
16	0.0	3.190627213497629E-4	0.0	3.190627213497629E-4	0.0
17	0.0	3.190627213497629E-4	0.0	6.381254426995258E-4	0.0
18	0.0	3.190627213497629E-4	0.0	6.381254426995258E-4	3.190627213497629E-4
19	0.0	3.190627213497629E-4	0.0	9.571881640492888E-4	3.190627213497629E-4
20	0.0	3.190627213497629E-4	0.0	9.571881640492888E-4	3.190627213497629E-4
21	0.0	3.190627213497629E-4	0.0	0.0015953136067488147	3.190627213497629E-4
22	0.0	3.190627213497629E-4	0.0	0.0025525017707981033	3.190627213497629E-4
23	0.0	3.190627213497629E-4	0.0	0.003828752656197155	3.190627213497629E-4
24	0.0	3.190627213497629E-4	0.0	0.006700317148345022	3.190627213497629E-4
25	0.0	3.190627213497629E-4	0.0	0.010210007083192413	3.190627213497629E-4
26	0.0	3.190627213497629E-4	0.0	0.018186575116936488	3.190627213497629E-4
27	0.0	3.190627213497629E-4	0.0	0.05966472889240567	3.190627213497629E-4
28	0.0	3.190627213497629E-4	0.0	0.2523786125876625	3.190627213497629E-4
29	0.0	3.190627213497629E-4	0.0	0.5542119469845382	3.190627213497629E-4
30	0.0	3.190627213497629E-4	0.0	0.9249628291929628	3.190627213497629E-4
31	0.0	3.190627213497629E-4	0.0	2.0021185764697624	3.190627213497629E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATTAT	30	3.5935585E-4	30.833334	1
CCCTACA	25	0.0054903207	29.6	2
ATACCAT	25	0.0054903207	29.6	6
TACGTCC	25	0.0054903207	29.6	30
GGGTATC	40	5.9265636E-5	27.75	1
AAGACGG	75	4.6020432E-10	27.133333	5
AGACGGA	75	4.6020432E-10	27.133333	6
CAAGACG	80	9.713403E-10	25.4375	4
TAGGACA	45	1.3202139E-4	24.666668	4
CCATCGG	55	1.8969402E-5	23.545454	19
ATACACA	120	1.0913936E-11	21.583334	37
GCGAAAG	95	7.0940587E-9	21.421053	18
GGTATCA	225	0.0	21.377777	1
CGTCGTA	70	5.082409E-6	21.142857	10
ACCGTCG	70	5.082409E-6	21.142857	8
TACCGTC	70	5.082409E-6	21.142857	7
ATACCGT	80	6.92693E-7	20.8125	6
CGGACCA	120	2.3646862E-10	20.041668	9
AAGCATT	105	2.2437234E-8	19.38095	22
GAGCGAA	105	2.2437234E-8	19.38095	16
>>END_MODULE