FastQCFastQC Report
Fri 10 Feb 2017
ERR1631245.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631245.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences770259
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12030.15618123254645513No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9380.12177722038950534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8420.10931388013642165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA400.001930611823.1250021
GTACTAT502.7009816E-422.1999991
GGTATCA5100.022.127451
ATAAGCG450.003824678520.55555722
GTCTAGG656.898351E-519.9230771
TACGCCC500.007032828418.49999835
TATACTC500.007032828418.4999983
TAGATTG752.066151E-417.2666665
TCTAGAT1301.3909812E-817.0769232
TATTAGA650.00157929417.0769232
ATACACA2600.017.07692337
CTTATAC8750.016.70285837
TAGAGTG1005.872729E-616.655
AGTCGGT1005.872729E-616.6511
CTAGATA1352.2158929E-816.4444453
TTTAACC904.4428918E-516.4444453
ATAACGA1708.54925E-1116.32352812
GCAGTCG1251.6555168E-716.2799999
GTCCTAA1151.2408182E-616.0869561
CATAGTA700.00259145315.8571423
Produced by FastQC (version 0.11.5)