##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631245.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	770259
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84451983034278	34.0	33.0	34.0	31.0	34.0
2	33.02316753196003	34.0	33.0	34.0	31.0	34.0
3	33.14431769054305	34.0	33.0	34.0	31.0	34.0
4	36.44750272311002	37.0	37.0	37.0	35.0	37.0
5	36.4288882051362	37.0	37.0	37.0	35.0	37.0
6	36.47035737329911	37.0	37.0	37.0	35.0	37.0
7	36.45989595707418	37.0	37.0	37.0	35.0	37.0
8	36.44727682506793	37.0	37.0	37.0	35.0	37.0
9	38.30143886666693	39.0	39.0	39.0	37.0	39.0
10	38.25709144586431	39.0	39.0	39.0	37.0	39.0
11	38.31365164185034	39.0	39.0	39.0	37.0	39.0
12	38.09184962460679	39.0	39.0	39.0	37.0	39.0
13	38.21735286442612	39.0	39.0	39.0	37.0	39.0
14	39.75312200182017	41.0	40.0	41.0	38.0	41.0
15	39.75958346478262	41.0	40.0	41.0	38.0	41.0
16	39.74215036760362	41.0	40.0	41.0	38.0	41.0
17	39.7276059091812	41.0	40.0	41.0	38.0	41.0
18	39.69912847496751	41.0	40.0	41.0	38.0	41.0
19	39.70470581973077	41.0	40.0	41.0	37.0	41.0
20	39.6745575189644	41.0	40.0	41.0	37.0	41.0
21	39.643812016477575	41.0	40.0	41.0	37.0	41.0
22	39.590942786776914	41.0	40.0	41.0	37.0	41.0
23	39.52539600316257	41.0	39.0	41.0	37.0	41.0
24	39.49039478928516	41.0	39.0	41.0	37.0	41.0
25	39.475215479468595	41.0	39.0	41.0	37.0	41.0
26	39.39926440327215	41.0	39.0	41.0	36.0	41.0
27	39.32974103515831	41.0	39.0	41.0	36.0	41.0
28	39.24526425526998	41.0	39.0	41.0	36.0	41.0
29	39.16419541998211	41.0	39.0	41.0	35.0	41.0
30	39.09836950947668	41.0	39.0	41.0	35.0	41.0
31	39.05225385227566	40.0	39.0	41.0	35.0	41.0
32	38.98285901235818	40.0	39.0	41.0	35.0	41.0
33	38.88169693570604	40.0	38.0	41.0	35.0	41.0
34	38.82934311705543	40.0	38.0	41.0	35.0	41.0
35	38.73320921923665	40.0	38.0	41.0	35.0	41.0
36	38.662439517097496	40.0	38.0	41.0	35.0	41.0
37	38.596037177105366	40.0	38.0	41.0	35.0	41.0
38	38.49271219161347	40.0	38.0	41.0	35.0	41.0
39	38.40296965046822	40.0	38.0	41.0	35.0	41.0
40	38.30394711389286	40.0	37.0	41.0	34.0	41.0
41	38.20614624431522	40.0	37.0	41.0	34.0	41.0
42	38.09585866572153	40.0	37.0	41.0	34.0	41.0
43	37.14808914923422	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	0.0
13	1.0
14	1.0
15	0.0
16	5.0
17	7.0
18	15.0
19	29.0
20	55.0
21	110.0
22	199.0
23	343.0
24	549.0
25	811.0
26	1238.0
27	1783.0
28	2495.0
29	3337.0
30	4563.0
31	6240.0
32	7968.0
33	10897.0
34	15949.0
35	23994.0
36	39150.0
37	73476.0
38	165911.0
39	411128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.316781757824316	16.781627997855267	11.351376614878891	27.55021362944153
2	20.50219471632269	19.11551828670616	31.560682835254116	28.821604161717033
3	20.733934949153465	20.165295050106522	27.984223488462973	31.116546512277036
4	15.532048311022656	14.62391221654015	31.815532178137484	38.02850729429971
5	17.729750642316418	33.15417281719525	31.30596331883172	17.81011322165661
6	37.05377022533979	32.101539871653564	14.972366437782616	15.872323465224037
7	32.45155201042766	28.185843982348796	18.975954841163816	20.38664916605973
8	29.89916378776489	29.91733949230064	18.700852570369186	21.482644149565274
9	28.24569398085579	13.331749450509504	18.080931219239243	40.341625349395464
10	19.33466535282288	23.83652771340549	29.596797960166644	27.232008973604984
11	39.586294999474205	19.364914918228806	19.454235523375903	21.59455455892109
12	24.446841906423682	23.208816774617368	26.058507592900572	26.285833726058378
13	32.171905813499095	16.95598493493747	22.60434477234281	28.267764479220624
14	24.64313951540975	18.688259403655135	22.323010831421637	34.34559024951348
15	28.106520014696358	24.737653178995636	19.68909159126995	27.466735215038057
16	27.96721622207595	23.519621322178644	21.324775173026218	27.18838728271919
17	27.09465257789912	23.5236459424687	22.622260823956616	26.759440655675558
18	26.973394663353496	22.009350101719033	23.831724134349617	27.185531100577858
19	27.32223836397887	22.903464938416818	23.03017556432317	26.744121133281144
20	27.43765408778086	22.04037862589077	22.867892488111142	27.65407479821722
21	28.52443139255757	22.52476115176843	22.387664408984513	26.56314304668949
22	28.501452108965946	22.356116578968894	22.275364520245788	26.867066791819376
23	27.189036415024038	22.080235349408444	22.85516949493612	27.875558740631394
24	27.193969820540882	22.814663639113597	22.804667001618935	27.186699538726582
25	27.43882252592959	21.696078851399335	22.68964075719985	28.175457865471227
26	27.242395090482553	22.961627192931207	22.784024594324766	27.011953122261474
27	27.701201803549196	22.190458014771654	22.653419174589327	27.454921007089823
28	26.73204727241097	22.55514054363532	23.328127292248453	27.38468489170526
29	26.173014531475776	22.83842188147104	23.779274244117886	27.2092893429353
30	25.82884458344531	23.35902598995922	23.62698780540052	27.185141621194948
31	26.907442821180926	22.821414615084016	22.468805947090527	27.80233661664453
32	26.219881883885808	22.649913860143148	23.116120681485057	28.014083574485987
33	25.840009659088697	22.192924717530076	24.047365886020156	27.91969973736107
34	27.189685547328885	22.086986325378867	23.512610693286284	27.210717434005964
35	26.739836860069143	21.97974966861796	24.319092668829576	26.961320802483318
36	25.805735473392716	22.438037075840725	24.112798422348845	27.643429028417714
37	26.042798591123244	21.574561283931768	24.77322562930131	27.609414495643676
38	25.30746151619131	21.049413249309648	25.89219989639848	27.75092533810056
39	25.358872794735277	20.65123549351582	26.321276349903084	27.668615361845823
40	24.84969341481242	20.864540368888907	27.12035821717111	27.165407999127567
41	23.94298541140058	20.795083212270157	27.926970019175368	27.33496135715389
42	24.268460349051423	20.945032774690073	28.035375114085003	26.751131762173504
43	23.228420570223783	19.993145162860802	28.435110787410466	28.343323479504946
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	50.5
2	52.0
3	110.0
4	168.0
5	168.0
6	236.5
7	305.0
8	280.5
9	256.0
10	380.5
11	505.0
12	505.0
13	829.0
14	1153.0
15	1810.5
16	2468.0
17	2282.0
18	2096.0
19	2096.0
20	2428.0
21	2760.0
22	2578.0
23	2396.0
24	2828.0
25	3260.0
26	3260.0
27	3857.0
28	4454.0
29	5517.5
30	6581.0
31	8111.5
32	9642.0
33	9642.0
34	12066.0
35	14490.0
36	17585.5
37	20681.0
38	23797.5
39	26914.0
40	26914.0
41	29818.5
42	32723.0
43	35475.5
44	38228.0
45	40756.0
46	43284.0
47	43284.0
48	47459.0
49	51634.0
50	53756.5
51	55879.0
52	58948.0
53	62017.0
54	62017.0
55	61721.0
56	61425.0
57	60299.5
58	59174.0
59	58185.0
60	57196.0
61	57196.0
62	54922.5
63	52649.0
64	48939.0
65	45229.0
66	40001.5
67	34774.0
68	34774.0
69	30443.5
70	26113.0
71	22819.5
72	19526.0
73	15507.0
74	11488.0
75	11488.0
76	9381.0
77	7274.0
78	6108.0
79	4942.0
80	4282.5
81	3623.0
82	3623.0
83	2769.5
84	1916.0
85	1672.0
86	1428.0
87	1193.0
88	958.0
89	958.0
90	699.0
91	440.0
92	249.5
93	59.0
94	50.0
95	41.0
96	41.0
97	23.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	770259.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.30831860844912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.64664679462078	52.858218998521956
2	6.75631500386364	8.14923995744107
3	1.9562885769456029	3.539414243655148
4	0.9051965316617008	2.1836352333886775
5	0.5574312150688151	1.680886966033251
6	0.3661799363137881	1.3250217760340124
7	0.25115438182165545	1.0602688935165936
8	0.18893339843771284	0.9115404471006918
9	0.14477233872066736	0.785787869633069
>10	1.0217495097572935	12.182178544108812
>50	0.12091109045365878	5.062554878145208
>100	0.08118255651673553	8.95470170651391
>500	0.003022754763495091	1.1499050569115163
>1k	2.159110545353636E-4	0.15664542899609943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1203	0.15618123254645513	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	938	0.12177722038950534	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	842	0.10931388013642165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.2982646096962192E-4
11	0.0	0.0	0.0	0.0	1.2982646096962192E-4
12	0.0	0.0	0.0	2.5965292193924383E-4	1.2982646096962192E-4
13	0.0	0.0	0.0	3.894793829088657E-4	1.2982646096962192E-4
14	0.0	0.0	0.0	5.193058438784877E-4	1.2982646096962192E-4
15	0.0	0.0	0.0	5.193058438784877E-4	1.2982646096962192E-4
16	0.0	0.0	0.0	7.789587658177314E-4	1.2982646096962192E-4
17	0.0	0.0	0.0	7.789587658177314E-4	1.2982646096962192E-4
18	0.0	0.0	0.0	7.789587658177314E-4	1.2982646096962192E-4
19	0.0	0.0	0.0	9.087852267873534E-4	1.2982646096962192E-4
20	0.0	0.0	0.0	0.0010386116877569753	1.2982646096962192E-4
21	0.0	0.0	0.0	0.001298264609696219	1.2982646096962192E-4
22	0.0	0.0	0.0	0.0022070498364835725	1.2982646096962192E-4
23	0.0	1.2982646096962192E-4	0.0	0.002986008602301304	1.2982646096962192E-4
24	0.0	1.2982646096962192E-4	0.0	0.00545271136072412	1.2982646096962192E-4
25	0.0	1.2982646096962192E-4	0.0	0.008698372884964667	1.2982646096962192E-4
26	0.0	1.2982646096962192E-4	0.0	0.01622830762120274	1.2982646096962192E-4
27	0.0	1.2982646096962192E-4	0.0	0.050242840395243675	1.2982646096962192E-4
28	0.0	1.2982646096962192E-4	0.0	0.1743569370822022	1.2982646096962192E-4
29	0.0	1.2982646096962192E-4	0.0	0.33495226930162453	1.2982646096962192E-4
30	0.0	1.2982646096962192E-4	0.0	0.5273550844586041	1.2982646096962192E-4
31	0.0	1.2982646096962192E-4	0.0	0.9981058319344532	1.2982646096962192E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAA	40	0.0019306118	23.125002	1
GTACTAT	50	2.7009816E-4	22.199999	1
GGTATCA	510	0.0	22.12745	1
ATAAGCG	45	0.0038246785	20.555557	22
GTCTAGG	65	6.898351E-5	19.923077	1
TACGCCC	50	0.0070328284	18.499998	35
TATACTC	50	0.0070328284	18.499998	3
TAGATTG	75	2.066151E-4	17.266666	5
TCTAGAT	130	1.3909812E-8	17.076923	2
TATTAGA	65	0.001579294	17.076923	2
ATACACA	260	0.0	17.076923	37
CTTATAC	875	0.0	16.702858	37
TAGAGTG	100	5.872729E-6	16.65	5
AGTCGGT	100	5.872729E-6	16.65	11
CTAGATA	135	2.2158929E-8	16.444445	3
TTTAACC	90	4.4428918E-5	16.444445	3
ATAACGA	170	8.54925E-11	16.323528	12
GCAGTCG	125	1.6555168E-7	16.279999	9
GTCCTAA	115	1.2408182E-6	16.086956	1
CATAGTA	70	0.002591453	15.857142	3
>>END_MODULE