FastQCFastQC Report
Fri 10 Feb 2017
ERR1631244.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631244.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43167
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9552.212338128663099No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8692.0131118678620243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6011.3922672411796047No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2610.604628535686983No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.33358815762040445No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.2548242870711423No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1090.25250770264322286No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1030.238608196075706No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.18532675423355804No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA760.17606041652188015No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA700.16216090995436327No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.141311650103088No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.11814580582389325No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.11582922139597378No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA500.11582922139597378No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.10887946811221536No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT450.10424629925637639No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA450.10424629925637639No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.10192971482845692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCCC200.00182237637.037
AGTCGGT556.0304046E-726.9090911
GGGCAGT601.2882956E-624.6666687
TGATTCC652.584935E-622.7692317
GTGATTC652.584935E-622.7692316
GATTCCT652.584935E-622.7692318
TTATACA502.6441689E-422.19999937
GGTGATT704.916874E-621.14285915
ATTCCTC704.916874E-621.14285919
GGCAGTC704.916874E-621.1428598
GTCGGTG704.916874E-621.14285912
TCCTCGC758.931778E-619.73333421
CAGTCGG758.931778E-619.73333410
TCGGTGA758.931778E-619.73333413
TTCCTCG758.931778E-619.73333420
GCAGTCG758.931778E-619.7333349
CGGTGAT758.931778E-619.73333414
TGGGCAG801.5587584E-518.56
ACCTGTC752.0097978E-417.26666636
CTCGCCT752.0097978E-417.26666623
Produced by FastQC (version 0.11.5)