FastQCFastQC Report
Fri 10 Feb 2017
ERR1631243.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631243.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030680
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT103691.006034850778127No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72690.7052625451158459No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70990.688768579966624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38360.3721814724259712No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC18620.1806574300461831No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA14490.14058679706601468No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT13730.13321302441106844No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG12160.11798036247914No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC11680.11332324290759498No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.11264407963674467No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11430.11089765979741531No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC11290.10953933325571469No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC10800.1047851903597625No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT10500.10187449062754686No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10390.10080723405906782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC400.001931052523.1258
GGACCGT400.001931052523.1256
GCAGTCG6900.021.9855089
CAGTCGG7150.021.21678410
AGTCGGT7150.021.21678411
GGCAGTC7300.020.780828
TCGGTGA7250.020.66896413
CGGTGAT7550.020.3377514
GTGATTC7500.020.22666716
TACTGGA656.901249E-519.9230772
GGGCAGT7800.019.6858987
GTCGGTG7750.019.57419212
TCCTCGC6200.018.79838821
TTCCTCG6300.018.7936520
AGCGTCA1409.458745E-1118.53
ACGAGTG500.00703440318.4999985
TTACAAC500.00703440318.4999983
GATTCCT8100.018.0432118
GGTGATT8550.017.74269115
AGGCCCG3550.017.71831110
Produced by FastQC (version 0.11.5)