##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631243.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1030680
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8267192533085	34.0	33.0	34.0	31.0	34.0
2	32.98254162300617	34.0	33.0	34.0	31.0	34.0
3	33.078992509799356	34.0	33.0	34.0	31.0	34.0
4	36.41581480187837	37.0	37.0	37.0	35.0	37.0
5	36.40946462529592	37.0	37.0	37.0	35.0	37.0
6	36.46534908992122	37.0	37.0	37.0	35.0	37.0
7	36.457937478169754	37.0	37.0	37.0	35.0	37.0
8	36.45905324640044	37.0	37.0	37.0	35.0	37.0
9	38.2993945744557	39.0	39.0	39.0	37.0	39.0
10	38.26871676951139	39.0	39.0	39.0	37.0	39.0
11	38.32243179260294	39.0	39.0	39.0	37.0	39.0
12	38.12434994372647	39.0	39.0	39.0	37.0	39.0
13	38.23811464276012	39.0	39.0	39.0	37.0	39.0
14	39.73960783172274	41.0	40.0	41.0	38.0	41.0
15	39.7257199130671	41.0	40.0	41.0	38.0	41.0
16	39.701205029689135	41.0	40.0	41.0	37.0	41.0
17	39.72217856172624	41.0	40.0	41.0	38.0	41.0
18	39.70614739783444	41.0	40.0	41.0	38.0	41.0
19	39.7251959871153	41.0	40.0	41.0	37.0	41.0
20	39.696092870726126	41.0	40.0	41.0	37.0	41.0
21	39.666535685178715	41.0	40.0	41.0	37.0	41.0
22	39.59028505452711	41.0	40.0	41.0	37.0	41.0
23	39.50945492296348	41.0	40.0	41.0	36.0	41.0
24	39.47373481584973	41.0	39.0	41.0	36.0	41.0
25	39.46009721737106	41.0	39.0	41.0	36.0	41.0
26	39.35469689913455	41.0	39.0	41.0	36.0	41.0
27	39.27343404354407	41.0	39.0	41.0	35.0	41.0
28	39.15164939651492	41.0	39.0	41.0	35.0	41.0
29	39.03902860247604	41.0	39.0	41.0	35.0	41.0
30	38.94151725074708	40.0	38.0	41.0	35.0	41.0
31	38.844801490278265	40.0	38.0	41.0	35.0	41.0
32	38.724667209997286	40.0	38.0	41.0	35.0	41.0
33	38.5791079675554	40.0	38.0	41.0	35.0	41.0
34	38.496762331664534	40.0	38.0	41.0	35.0	41.0
35	38.34775196957349	40.0	38.0	41.0	35.0	41.0
36	38.250103814957114	40.0	38.0	41.0	35.0	41.0
37	38.13496526564986	40.0	37.0	41.0	35.0	41.0
38	37.99001144875228	40.0	37.0	41.0	34.0	41.0
39	37.84563103970194	40.0	37.0	41.0	34.0	41.0
40	37.67354465013389	40.0	36.0	41.0	33.0	41.0
41	37.50169208677766	40.0	36.0	41.0	33.0	41.0
42	37.3012438390189	40.0	36.0	41.0	33.0	41.0
43	36.365277292661155	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	5.0
12	8.0
13	4.0
14	4.0
15	6.0
16	4.0
17	17.0
18	21.0
19	46.0
20	80.0
21	172.0
22	284.0
23	477.0
24	809.0
25	1262.0
26	1982.0
27	2933.0
28	4292.0
29	6136.0
30	8476.0
31	10646.0
32	12887.0
33	16875.0
34	24890.0
35	36485.0
36	54760.0
37	115196.0
38	203743.0
39	528173.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.67576745449606	16.893022082508637	12.265688671556642	25.16552179143866
2	19.66740404393216	20.740579035200064	32.754298133271234	26.837718787596536
3	20.226452439166376	23.009081383164514	29.26116738463927	27.503298793029845
4	13.939341017580626	15.443784685838475	33.04633833973688	37.570535956844026
5	15.081693639150851	36.71265572243567	31.509779951100242	16.69587068731323
6	33.63915085186479	33.708619552140334	16.755443008499242	15.896786587495635
7	29.834963325183374	30.57573640703225	20.11245003298793	19.476850234796444
8	30.77531338533783	30.107113750145537	19.054895796949587	20.06267706756704
9	28.72918849691466	13.241258198470913	18.825823728024215	39.20372957659021
10	19.585807428105717	25.741840338417354	28.58181006714014	26.09054216633679
11	36.437982691038926	22.299355765125934	19.517600031047465	21.745061512787675
12	24.173167229401948	26.154286490472312	26.16156323980285	23.510983040322895
13	30.964411844607444	18.062735281561686	25.21772033996973	25.75513253386114
14	23.548045950246436	20.297570535956844	24.756568479062366	31.39781503473435
15	26.923875499670118	26.207261225598632	21.94075755811697	24.928105716614272
16	25.204234097877134	24.980498311794154	23.503027127721506	26.31224046260721
17	23.95748437924477	26.382485349478014	24.65188031202701	25.008149959250204
18	24.244382349516826	22.884794504598904	27.069216439632086	25.801606706252183
19	24.7354173943416	24.824193736174177	26.622132960763768	23.818255908720456
20	25.099739977490586	23.498370008149962	26.66230061706834	24.73958939729111
21	28.084274459580083	23.584720766872355	25.482982108898977	22.848022664648582
22	26.93115224900066	23.726180773858037	25.075678193037604	24.2669887841037
23	24.8164318702216	24.17142080956262	25.560018628478286	25.452128691737492
24	24.18180230527419	25.062871114215856	26.129060426126436	24.626266154383515
25	24.25515193852602	23.92992975511313	26.14215857492141	25.672759731439438
26	24.846994217409865	25.111285753095043	26.30603096984515	23.73568905964994
27	25.72243567353592	23.965537315170568	25.335118562502423	24.97690844879109
28	23.692319633639926	24.312686769899482	27.283735009896382	24.71125858656421
29	23.6001474754531	24.23244848061474	27.70219660806458	24.465207435867583
30	22.777292661155744	25.16348430162611	27.902161679667792	24.157061357550354
31	23.826599914619475	25.203069817984243	26.289634028020338	24.680696239375948
32	23.121240346179224	24.636356580121863	27.103756743121043	25.138646330577867
33	23.132786121783678	24.361392478751892	27.530950440485892	24.97487095897854
34	24.253114448713468	24.455214033453643	26.880312027011293	24.411359490821592
35	23.987658633135407	24.265436410913182	27.664357511545774	24.082547444405638
36	22.50164939651492	24.992626227345056	26.789886288663794	25.71583808747623
37	22.67066402763224	23.688244654014827	28.03091163115613	25.610179687196805
38	22.505918422788838	22.583634105638993	29.29551364147941	25.614933830092756
39	22.422963480420695	22.692591298948265	29.303663600729617	25.580781619901426
40	22.04884154150658	22.542981332712383	30.19909186168355	25.209085264097492
41	20.229266115574184	23.022470601932703	31.310979159389916	25.437284123103193
42	20.967807660961697	23.101544611324563	30.807816199014244	25.122831528699503
43	19.667015950634532	22.154305895137192	31.419160166103932	26.759517988124344
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	311.0
1	289.0
2	267.0
3	970.5
4	1674.0
5	1674.0
6	2297.0
7	2920.0
8	2733.5
9	2547.0
10	3818.0
11	5089.0
12	5089.0
13	8332.5
14	11576.0
15	16439.0
16	21302.0
17	18316.5
18	15331.0
19	15331.0
20	16101.5
21	16872.0
22	12403.0
23	7934.0
24	7984.0
25	8034.0
26	8034.0
27	8691.5
28	9349.0
29	10854.5
30	12360.0
31	14305.5
32	16251.0
33	16251.0
34	19925.5
35	23600.0
36	28157.0
37	32714.0
38	37398.5
39	42083.0
40	42083.0
41	45815.5
42	49548.0
43	53072.5
44	56597.0
45	60591.0
46	64585.0
47	64585.0
48	69510.5
49	74436.0
50	74332.0
51	74228.0
52	75267.5
53	76307.0
54	76307.0
55	73020.5
56	69734.0
57	66584.5
58	63435.0
59	59974.0
60	56513.0
61	56513.0
62	53909.0
63	51305.0
64	47200.5
65	43096.0
66	38007.0
67	32918.0
68	32918.0
69	28410.0
70	23902.0
71	21498.5
72	19095.0
73	17473.5
74	15852.0
75	15852.0
76	14507.0
77	13162.0
78	10485.5
79	7809.0
80	5446.5
81	3084.0
82	3084.0
83	2409.5
84	1735.0
85	1566.5
86	1398.0
87	1256.5
88	1115.0
89	1115.0
90	799.0
91	483.0
92	281.5
93	80.0
94	56.5
95	33.0
96	33.0
97	23.5
98	14.0
99	8.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1030680.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.345833715948785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.12781357562102	40.38011160009917
2	6.358388204622035	5.893696048657258
3	1.9404641749166434	2.6979728994732755
4	0.9933072757722912	1.8414261532713878
5	0.6209605587369754	1.4389467399693259
6	0.3925218082228828	1.091505027226876
7	0.3058420128594695	0.992215214993523
8	0.2478896142449766	0.9190920673366699
9	0.1918901913677789	0.8003979810767404
>10	1.4393364200184202	13.330953462231673
>50	0.18942787514254877	6.181565613817083
>100	0.1751289434407086	16.053027756866108
>500	0.013665523792898308	4.165331638537107
>1k	0.0027331047585796623	1.8034602750399613
>5k	4.2047765516610186E-4	1.3999739171091992
>10k+	2.1023882758305093E-4	1.0103236042946329
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10369	1.006034850778127	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7269	0.7052625451158459	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7099	0.688768579966624	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3836	0.3721814724259712	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1862	0.1806574300461831	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1449	0.14058679706601468	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	1373	0.13321302441106844	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1216	0.11798036247914	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1168	0.11332324290759498	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1161	0.11264407963674467	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1143	0.11089765979741531	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1129	0.10953933325571469	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1080	0.1047851903597625	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1050	0.10187449062754686	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1039	0.10080723405906782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.702332440718748E-5	0.0
2	0.0	0.0	0.0	9.702332440718748E-5	0.0
3	0.0	0.0	0.0	9.702332440718748E-5	0.0
4	0.0	9.702332440718748E-5	0.0	9.702332440718748E-5	0.0
5	1.9404664881437497E-4	9.702332440718748E-5	0.0	1.9404664881437497E-4	0.0
6	1.9404664881437497E-4	9.702332440718748E-5	0.0	1.9404664881437497E-4	0.0
7	1.9404664881437497E-4	9.702332440718748E-5	0.0	1.9404664881437497E-4	0.0
8	1.9404664881437497E-4	9.702332440718748E-5	0.0	1.9404664881437497E-4	0.0
9	1.9404664881437497E-4	9.702332440718748E-5	0.0	5.821399464431249E-4	0.0
10	2.9106997322156245E-4	9.702332440718748E-5	0.0	9.702332440718748E-4	0.0
11	2.9106997322156245E-4	9.702332440718748E-5	0.0	0.0018434431637365622	0.0
12	2.9106997322156245E-4	9.702332440718748E-5	0.0	0.002813676407808437	0.0
13	2.9106997322156245E-4	9.702332440718748E-5	0.0	0.003201769705437187	0.0
14	2.9106997322156245E-4	9.702332440718748E-5	0.0	0.0038809329762874993	0.0
15	2.9106997322156245E-4	9.702332440718748E-5	0.0	0.004948189544766562	0.0
16	2.9106997322156245E-4	9.702332440718748E-5	0.0	0.0060154461132456245	0.0
17	2.9106997322156245E-4	9.702332440718748E-5	0.0	0.007567819303760624	0.0
18	2.9106997322156245E-4	9.702332440718748E-5	0.0	0.008538052547832499	0.0
19	2.9106997322156245E-4	9.702332440718748E-5	0.0	0.0100904257383475	0.0
20	2.9106997322156245E-4	9.702332440718748E-5	0.0	0.012613032172934373	0.0
21	2.9106997322156245E-4	9.702332440718748E-5	0.0	0.01581480187837156	0.0
22	3.8809329762874993E-4	9.702332440718748E-5	0.0	0.023091551208910623	0.0
23	3.8809329762874993E-4	9.702332440718748E-5	0.0	0.032599837000814993	0.0
24	3.8809329762874993E-4	9.702332440718748E-5	0.0	0.042690262739162495	0.0
25	4.851166220359374E-4	9.702332440718748E-5	0.0	0.05025808204292312	0.0
26	5.821399464431249E-4	9.702332440718748E-5	0.0	0.06316218418907905	0.0
27	5.821399464431249E-4	9.702332440718748E-5	0.0	0.12244343540187061	0.0
28	5.821399464431249E-4	9.702332440718748E-5	0.0	0.3202739938681259	0.0
29	6.791632708503124E-4	1.9404664881437497E-4	0.0	0.6520937633407071	0.0
30	6.791632708503124E-4	1.9404664881437497E-4	0.0	1.192707726937556	0.0
31	6.791632708503124E-4	1.9404664881437497E-4	0.0	2.0144952846664337	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCC	40	0.0019310525	23.125	8
GGACCGT	40	0.0019310525	23.125	6
GCAGTCG	690	0.0	21.985508	9
CAGTCGG	715	0.0	21.216784	10
AGTCGGT	715	0.0	21.216784	11
GGCAGTC	730	0.0	20.78082	8
TCGGTGA	725	0.0	20.668964	13
CGGTGAT	755	0.0	20.33775	14
GTGATTC	750	0.0	20.226667	16
TACTGGA	65	6.901249E-5	19.923077	2
GGGCAGT	780	0.0	19.685898	7
GTCGGTG	775	0.0	19.574192	12
TCCTCGC	620	0.0	18.798388	21
TTCCTCG	630	0.0	18.79365	20
AGCGTCA	140	9.458745E-11	18.5	3
ACGAGTG	50	0.007034403	18.499998	5
TTACAAC	50	0.007034403	18.499998	3
GATTCCT	810	0.0	18.04321	18
GGTGATT	855	0.0	17.742691	15
AGGCCCG	355	0.0	17.718311	10
>>END_MODULE