FastQCFastQC Report
Fri 10 Feb 2017
ERR1631242.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631242.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169975
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6240.367112810707457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4840.28474775702309163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4000.2353287248124724No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3780.22238564494778645No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC3170.1864980144138844No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC2970.1747315781732608No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC2840.16708339461685542No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC2430.14296220032357698No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC2040.12001764965436094No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC2010.1182526842182674No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT1840.10825121341373732No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG1830.10766289160170613No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1820.10707456978967496No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.10589792616561258No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA1790.10530960435358142No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC1770.10413296072951904No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC1750.10295631710545668No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGA200.001837526237.033
GAGTTCC251.2292039E-437.015
AATACCC200.001837526237.05
GCCGAGT303.5866984E-430.83333412
CCGAGTT303.5866984E-430.83333413
AGATTTG303.5866984E-430.8333346
ATGAATT303.5866984E-430.83333420
AAAGTGA250.005483336329.625
TTTGGAT250.005483336329.634
GGGTAAG405.910844E-527.7500021
CGAGTTC358.838203E-426.4285714
CCCTAAG358.838203E-426.428571
TGCATAA358.838203E-426.4285714
TCCTTTT358.838203E-426.4285719
ATACACA706.508344E-926.4285737
GTTCCTT451.3167413E-424.66666817
GTAAGAT451.3167413E-424.6666683
GATTTGC400.001924459523.1250027
CTGCATA400.001924459523.12500213
TTTGCCG400.001924459523.1250029
Produced by FastQC (version 0.11.5)