##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631242.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	169975
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8027239299897	34.0	31.0	34.0	31.0	34.0
2	32.97177231945874	34.0	33.0	34.0	31.0	34.0
3	33.06784527136344	34.0	33.0	34.0	31.0	34.0
4	36.42318576261215	37.0	37.0	37.0	35.0	37.0
5	36.41415502279747	37.0	37.0	37.0	35.0	37.0
6	36.46455655243418	37.0	37.0	37.0	35.0	37.0
7	36.45087218708634	37.0	37.0	37.0	35.0	37.0
8	36.448183556405354	37.0	37.0	37.0	35.0	37.0
9	38.29547286365642	39.0	39.0	39.0	37.0	39.0
10	38.24418296808354	39.0	39.0	39.0	37.0	39.0
11	38.31416384762465	39.0	39.0	39.0	37.0	39.0
12	38.08055302250331	39.0	39.0	39.0	37.0	39.0
13	38.21516105309605	39.0	39.0	39.0	37.0	39.0
14	39.69139579349904	41.0	40.0	41.0	37.0	41.0
15	39.701385497867335	41.0	40.0	41.0	38.0	41.0
16	39.659520517723195	41.0	40.0	41.0	37.0	41.0
17	39.65903809383733	41.0	40.0	41.0	37.0	41.0
18	39.677952640094134	41.0	40.0	41.0	37.0	41.0
19	39.69714369760259	41.0	40.0	41.0	37.0	41.0
20	39.67076923076923	41.0	40.0	41.0	37.0	41.0
21	39.64380644212384	41.0	40.0	41.0	37.0	41.0
22	39.583732901897335	41.0	40.0	41.0	37.0	41.0
23	39.489960288277686	41.0	39.0	41.0	37.0	41.0
24	39.47561700250037	41.0	39.0	41.0	37.0	41.0
25	39.45081923812325	41.0	39.0	41.0	37.0	41.0
26	39.3861273716723	41.0	39.0	41.0	36.0	41.0
27	39.299838211501694	41.0	39.0	41.0	36.0	41.0
28	39.180879541108986	41.0	39.0	41.0	36.0	41.0
29	39.10166200911899	41.0	39.0	41.0	35.0	41.0
30	39.00427121635535	40.0	39.0	41.0	35.0	41.0
31	38.941626709810265	40.0	39.0	41.0	35.0	41.0
32	38.855508162965144	40.0	38.0	41.0	35.0	41.0
33	38.74837476099427	40.0	38.0	41.0	35.0	41.0
34	38.69247830563318	40.0	38.0	41.0	35.0	41.0
35	38.565124282982794	40.0	38.0	41.0	35.0	41.0
36	38.517017208413	40.0	38.0	41.0	35.0	41.0
37	38.434463891748784	40.0	38.0	41.0	35.0	41.0
38	38.326247977643774	40.0	38.0	41.0	34.0	41.0
39	38.21458449772025	40.0	37.0	41.0	34.0	41.0
40	38.11658773349022	40.0	37.0	41.0	34.0	41.0
41	37.99452860714811	40.0	37.0	41.0	34.0	41.0
42	37.86170319164583	40.0	37.0	41.0	34.0	41.0
43	36.91416384762465	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	3.0
18	4.0
19	3.0
20	23.0
21	16.0
22	43.0
23	67.0
24	136.0
25	217.0
26	305.0
27	389.0
28	607.0
29	825.0
30	1088.0
31	1444.0
32	1861.0
33	2481.0
34	3744.0
35	5392.0
36	8982.0
37	17564.0
38	38337.0
39	86441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.73451978232093	18.148551257537875	12.903073981467864	26.213854978673336
2	20.651272245918516	18.56155316958376	32.98543903515223	27.80173554934549
3	19.663479923518164	21.502573907927637	29.034269745550816	29.799676423003383
4	13.76320047065745	16.39770554493308	34.33093101926754	35.508162965141935
5	15.290483894690396	35.82232681276658	32.82012060597147	16.067068686571552
6	32.813649066039126	35.141638476246506	16.264156493602	15.78055596411237
7	30.99455802323871	29.526695102220913	19.669363141638478	19.809383732901896
8	30.241506103838802	30.335049271951757	17.89851448742462	21.52493013678482
9	28.013531401676715	13.204883071039857	18.72510663332843	40.056478893954996
10	20.70069127812914	23.379320488307105	28.456537726136194	27.463450507427563
11	38.35917046624503	21.8132078246801	18.500367701132518	21.327254007942344
12	24.29827915869981	25.993822620973674	24.42359170466245	25.284306515664067
13	32.39476393587292	17.431975290483894	23.805853802029713	26.367406971613473
14	23.769377849683774	19.631122223856448	22.833357846742167	33.7661420797176
15	29.1942932784233	24.877776143550523	20.036475952345935	25.89145462568025
16	27.291072216502428	24.090013237240772	22.270333872628328	26.348580673628476
17	25.62317987939403	25.390204441829685	22.402117958523313	26.58449772025298
18	25.87792322400353	21.982056184733047	24.45418443888807	27.685836152375348
19	26.33269598470363	24.683629945580233	23.754081482570967	25.229592587145167
20	26.99043977055449	22.099132225327253	24.357699661714957	26.55272834240329
21	29.351963524047655	22.68157081923812	23.15987645241947	24.80658920429475
22	30.00147080453008	21.455508162965142	22.19738196793646	26.34563906456832
23	26.859832328283574	22.137961464921315	23.677011325194883	27.325194881600233
24	25.174290336814238	23.801147227533463	23.45639064568319	27.568171789969114
25	26.3509339608766	21.980879541108987	23.765847918811588	27.902338579202823
26	26.273275481688486	23.623474040300042	23.546403882923958	26.556846595087514
27	27.288130607442273	22.35681717899691	23.124577143697604	27.230475069863214
28	25.520223562288574	22.62979849977938	24.99602882776879	26.85394911016326
29	25.57787909986763	22.936902485659655	25.702015002206206	25.78320341226651
30	24.69304309457273	23.93057802618032	25.737314310928078	25.63906456831887
31	26.249154287395204	23.53228415943521	23.474040300044123	26.74452125312546
32	24.560670686865716	22.493896161200176	25.800853066627443	27.144580085306664
33	24.051772319458745	22.183850566259743	26.247389321959112	27.5169877923224
34	26.294455066921607	21.5584644800706	25.550816296514196	26.596264156493604
35	25.224886012648916	22.25033093101927	26.53154875717017	25.993234299161642
36	23.640535372848948	22.87395205177232	25.790851595822918	27.69466097955582
37	24.71775261067804	20.804824238858654	27.35166936314164	27.125753787321667
38	23.263421091336962	19.847624650683922	28.57949698485071	28.3094572731284
39	23.437564347698192	19.931754669804384	29.695543462273864	26.935137520223563
40	22.735696425944994	20.05412560670687	30.168554199146936	27.041623768201205
41	20.829533754963965	20.30828062950434	31.868215914105015	26.99396970142668
42	21.26077364318282	20.488895425797914	31.559935284600677	26.69039564641859
43	19.295190469186647	20.08471834093249	32.016473010736874	28.603618179143993
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	3.5
2	7.0
3	23.5
4	40.0
5	40.0
6	61.0
7	82.0
8	90.0
9	98.0
10	138.0
11	178.0
12	178.0
13	325.0
14	472.0
15	802.0
16	1132.0
17	965.5
18	799.0
19	799.0
20	861.0
21	923.0
22	625.5
23	328.0
24	323.5
25	319.0
26	319.0
27	397.5
28	476.0
29	800.0
30	1124.0
31	1542.0
32	1960.0
33	1960.0
34	2791.5
35	3623.0
36	4364.0
37	5105.0
38	5866.5
39	6628.0
40	6628.0
41	7766.5
42	8905.0
43	10132.0
44	11359.0
45	12213.0
46	13067.0
47	13067.0
48	14130.0
49	15193.0
50	14899.5
51	14606.0
52	14554.0
53	14502.0
54	14502.0
55	13033.0
56	11564.0
57	10716.0
58	9868.0
59	9898.0
60	9928.0
61	9928.0
62	9668.0
63	9408.0
64	8652.5
65	7897.0
66	7305.0
67	6713.0
68	6713.0
69	5755.5
70	4798.0
71	4100.5
72	3403.0
73	2641.5
74	1880.0
75	1880.0
76	1527.0
77	1174.0
78	1000.0
79	826.0
80	726.5
81	627.0
82	627.0
83	501.0
84	375.0
85	318.5
86	262.0
87	217.5
88	173.0
89	173.0
90	142.5
91	112.0
92	70.0
93	28.0
94	20.0
95	12.0
96	12.0
97	6.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	169975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.331813501985586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.48373746226507	38.899249889689656
2	8.178741844386016	7.905868510075011
3	3.240334988801247	4.6983379908810115
4	1.8770084721004967	3.6287689366083247
5	1.141785957736878	2.759229298426239
6	0.8350374914792094	2.421532578320341
7	0.5879345603271984	1.9891160464774231
8	0.5258545135845749	2.0332401823797617
9	0.37613204791118904	1.6361229592587143
>10	2.516067776803973	22.704515369907337
>50	0.1691985587691109	5.725547874687454
>100	0.06694907001655467	5.231357552581262
>500	0.0012172558184828123	0.367112810707457
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	624	0.367112810707457	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	484	0.28474775702309163	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	400	0.2353287248124724	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	378	0.22238564494778645	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	317	0.1864980144138844	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	297	0.1747315781732608	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	284	0.16708339461685542	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	243	0.14296220032357698	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	204	0.12001764965436094	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	201	0.1182526842182674	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	184	0.10825121341373732	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	183	0.10766289160170613	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	182	0.10707456978967496	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	180	0.10589792616561258	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	179	0.10530960435358142	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	177	0.10413296072951904	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	175	0.10295631710545668	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.883218120311811E-4
7	0.0	0.0	0.0	0.0	5.883218120311811E-4
8	0.0	0.0	0.0	0.0	5.883218120311811E-4
9	0.0	0.0	0.0	0.0	5.883218120311811E-4
10	0.0	0.0	0.0	0.0	5.883218120311811E-4
11	0.0	0.0	0.0	0.0	5.883218120311811E-4
12	0.0	0.0	0.0	0.0	5.883218120311811E-4
13	0.0	0.0	0.0	0.0	5.883218120311811E-4
14	0.0	0.0	0.0	5.883218120311811E-4	0.0011766436240623621
15	0.0	0.0	0.0	0.001764965436093543	0.0011766436240623621
16	0.0	0.0	0.0	0.001764965436093543	0.0011766436240623621
17	0.0	0.0	0.0	0.0023532872481247242	0.0011766436240623621
18	0.0	0.0	0.0	0.003529930872187086	0.0011766436240623621
19	0.0	0.0	0.0	0.003529930872187086	0.0011766436240623621
20	0.0	0.0	0.0	0.00529489630828063	0.0011766436240623621
21	0.0	0.0	0.0	0.005883218120311811	0.0011766436240623621
22	0.0	0.0	0.0	0.008236505368436535	0.0011766436240623621
23	0.0	0.0	0.0	0.012354758052654803	0.0011766436240623621
24	0.0	0.0	0.0	0.020002941609060156	0.0011766436240623621
25	0.0	0.0	0.0	0.022944550669216062	0.001764965436093543
26	0.0	0.0	0.0	0.03235769966171496	0.001764965436093543
27	0.0	0.0	0.0	0.07236358287983527	0.001764965436093543
28	0.0	0.0	0.0	0.2765112516546551	0.001764965436093543
29	0.0	0.0	0.0	0.5871451684071187	0.001764965436093543
30	0.0	0.0	0.0	1.0189733784380055	0.001764965436093543
31	0.0	0.0	0.0	2.272098838064421	0.001764965436093543
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGGA	20	0.0018375262	37.0	33
GAGTTCC	25	1.2292039E-4	37.0	15
AATACCC	20	0.0018375262	37.0	5
GCCGAGT	30	3.5866984E-4	30.833334	12
CCGAGTT	30	3.5866984E-4	30.833334	13
AGATTTG	30	3.5866984E-4	30.833334	6
ATGAATT	30	3.5866984E-4	30.833334	20
AAAGTGA	25	0.0054833363	29.6	25
TTTGGAT	25	0.0054833363	29.6	34
GGGTAAG	40	5.910844E-5	27.750002	1
CGAGTTC	35	8.838203E-4	26.42857	14
CCCTAAG	35	8.838203E-4	26.42857	1
TGCATAA	35	8.838203E-4	26.42857	14
TCCTTTT	35	8.838203E-4	26.42857	19
ATACACA	70	6.508344E-9	26.42857	37
GTTCCTT	45	1.3167413E-4	24.666668	17
GTAAGAT	45	1.3167413E-4	24.666668	3
GATTTGC	40	0.0019244595	23.125002	7
CTGCATA	40	0.0019244595	23.125002	13
TTTGCCG	40	0.0019244595	23.125002	9
>>END_MODULE