FastQCFastQC Report
Fri 10 Feb 2017
ERR1631240.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631240.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1673936
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA31650.18907532904483804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28730.1716314124315386No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT26670.1593250876974986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25670.1533511436518481No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC25490.15227583372363102No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC24670.1473771996061976No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC24540.14660058688026303No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT23900.14277726269104674No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC22180.13250207893252788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20950.1251541277563778No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC20660.12342168398313914No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG19760.11804513434205369No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG18900.11290754246279429No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC18220.10884526051175195No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC17680.10561933072710068No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG17650.10544011240573117No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA17410.10400636583477504No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA17240.10299079534701447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17550.023.5071221
GTCTATA450.003826527220.5555551
CGGGTAG500.00703618918.510
ACGAATA500.00703618918.53
TTAGGCA953.6083784E-617.5263164
ATTACAC904.447908E-516.4444453
GTAATAC904.447908E-516.4444453
TATACAG904.447908E-516.4444455
CGATTAA1151.2429482E-616.08695624
GCATTAT1851.8189894E-1116.01
GCTAGAT1059.348112E-615.8571443
ACTAGAC700.00259315715.8571423
GTATCAA26200.015.6755732
TCTAGCG6500.015.65384628
CTAGCGG6550.015.53435129
CGCAAGA7900.015.4556962
ACGATTA1201.9366853E-615.41666723
CTTATAC17950.015.35654637
CGAACGA5350.015.21495416
ACGAACG5250.015.15238215
Produced by FastQC (version 0.11.5)