##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631240.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1673936
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86178025922138	34.0	33.0	34.0	31.0	34.0
2	33.03230649200447	34.0	33.0	34.0	31.0	34.0
3	33.14916639584787	34.0	33.0	34.0	31.0	34.0
4	36.47539690884239	37.0	37.0	37.0	35.0	37.0
5	36.453433106164155	37.0	37.0	37.0	35.0	37.0
6	36.49214904273521	37.0	37.0	37.0	35.0	37.0
7	36.48028478986055	37.0	37.0	37.0	35.0	37.0
8	36.46726637099626	37.0	37.0	37.0	35.0	37.0
9	38.31870095391938	39.0	39.0	39.0	37.0	39.0
10	38.280029224534275	39.0	39.0	39.0	37.0	39.0
11	38.33634977681345	39.0	39.0	39.0	37.0	39.0
12	38.11147260110303	39.0	39.0	39.0	37.0	39.0
13	38.24564917655155	39.0	39.0	39.0	37.0	39.0
14	39.78094144579004	41.0	40.0	41.0	38.0	41.0
15	39.78367512258533	41.0	40.0	41.0	38.0	41.0
16	39.76341747832653	41.0	40.0	41.0	38.0	41.0
17	39.75165239292303	41.0	40.0	41.0	38.0	41.0
18	39.7287214087038	41.0	40.0	41.0	38.0	41.0
19	39.73610878791065	41.0	40.0	41.0	38.0	41.0
20	39.71123925884861	41.0	40.0	41.0	38.0	41.0
21	39.677266633849804	41.0	40.0	41.0	37.0	41.0
22	39.62591998738303	41.0	40.0	41.0	37.0	41.0
23	39.54699880999035	41.0	40.0	41.0	37.0	41.0
24	39.511993887460456	41.0	39.0	41.0	37.0	41.0
25	39.49621550644708	41.0	39.0	41.0	37.0	41.0
26	39.426437450416266	41.0	39.0	41.0	36.0	41.0
27	39.35175060456314	41.0	39.0	41.0	36.0	41.0
28	39.24421244300857	41.0	39.0	41.0	36.0	41.0
29	39.16681820571396	41.0	39.0	41.0	35.0	41.0
30	39.109936700088895	41.0	39.0	41.0	35.0	41.0
31	39.06340803949494	41.0	39.0	41.0	35.0	41.0
32	38.98794458091588	40.0	39.0	41.0	35.0	41.0
33	38.87143475019356	40.0	39.0	41.0	35.0	41.0
34	38.83150371340362	40.0	38.0	41.0	35.0	41.0
35	38.71286417162902	40.0	38.0	41.0	35.0	41.0
36	38.65201955152407	40.0	38.0	41.0	35.0	41.0
37	38.5792903671347	40.0	38.0	41.0	35.0	41.0
38	38.4786556953193	40.0	38.0	41.0	35.0	41.0
39	38.39124972519858	40.0	38.0	41.0	35.0	41.0
40	38.285170998174365	40.0	37.0	41.0	34.0	41.0
41	38.190788058802724	40.0	37.0	41.0	34.0	41.0
42	38.06031293908489	40.0	37.0	41.0	34.0	41.0
43	37.12277112147657	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	2.0
14	2.0
15	5.0
16	14.0
17	30.0
18	49.0
19	81.0
20	171.0
21	312.0
22	529.0
23	836.0
24	1391.0
25	1809.0
26	2736.0
27	3841.0
28	5340.0
29	7224.0
30	9830.0
31	12907.0
32	16912.0
33	23000.0
34	34368.0
35	51363.0
36	84710.0
37	158566.0
38	354153.0
39	903752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.02713126427773	17.624927117882645	11.699133061240095	26.648808556599533
2	19.926329322029037	19.786359813039446	32.24693178233816	28.040379082593358
3	20.21140593188748	21.169865514571644	28.965504057502795	29.65322449603808
4	14.523554066583191	15.007443534280881	33.281857848806645	37.18714455032928
5	16.404749046558532	34.66942583228988	32.26150820580954	16.664316915342045
6	35.47005381328796	33.82160369333116	15.539184293784231	15.16915819959664
7	31.020361590885194	29.416357614628037	19.831224132822282	19.732056661664483
8	29.520304241022355	30.181799065197236	19.332340065570012	20.965556628210397
9	27.84455319677694	13.46604649162214	18.531652345131473	40.15774796646945
10	19.099057550587357	24.233602718383498	29.887462842068036	26.779876888961105
11	38.96415394614848	20.16283776679634	19.611980386346907	21.26102790070827
12	23.867638906146947	24.279602087535007	26.229079247952132	25.623679758365913
13	31.54606866690244	17.044976629930893	23.958681813402663	27.450272889764005
14	24.347525831334053	19.394469083644776	22.32247827873945	33.93552680628172
15	27.601951329083068	25.653848175796444	20.086251804131102	26.65794869098938
16	27.751837585188444	23.918716127737262	21.843308226837824	26.486138060236474
17	26.55400206459506	24.19698244138366	22.615559973619064	26.633455520402215
18	26.67443677655538	21.99719702545378	24.44537903480181	26.882987163189036
19	26.72611139255025	23.351549880043205	23.55245361829843	26.369885109108115
20	27.30164116190822	21.957291079228835	23.20494929316294	27.536118465700003
21	29.14060035748081	22.916168837996196	22.46262700605041	25.480603798472583
22	28.62702038787624	22.15604419762763	22.583599372974835	26.6333360415213
23	26.79158581929058	22.064105198765066	23.46714569732654	27.677163284617812
24	26.30978723200887	23.358838091778896	23.33745137209547	26.993923304116763
25	26.9543160550941	22.104608539394576	23.225499660679976	27.715575744831344
26	26.6255101748215	23.77313111134476	23.292587052312633	26.308771661521106
27	27.861280240104758	22.165124592577016	22.88809130098164	27.085503866336584
28	26.27394356773497	22.84932040412537	24.088794314716928	26.787941713422736
29	25.60438391909846	23.142282620124067	24.34125319008612	26.91208027069135
30	25.027778839812274	24.21394844247331	24.352603683772855	26.40566903394156
31	26.874205465441932	23.537817455386588	22.577864386691008	27.010112692480476
32	25.836591124152896	22.85863975683658	23.242764358971908	28.06200476003862
33	25.288959673488115	22.506834191988222	24.19566817369362	28.008537960830044
34	26.8110608768794	22.33705470221084	24.13616769099894	26.71571672991082
35	26.686085967444395	22.295177354450825	24.933928178855105	26.08480849924967
36	24.89354431710651	23.60830999512526	24.481879832920733	27.0162658548475
37	26.208349662113722	22.125099167471156	24.842347616635283	26.824203553779835
38	25.015830951720975	21.817500788560615	25.913655002341784	27.253013257376622
39	25.645902830215732	21.501060972462508	25.654923485724662	27.1981127115971
40	24.913079114135787	22.019420097303602	26.540919127135087	26.526581661425524
41	23.664823505797113	22.225222469676257	27.619514724577282	26.490439299949344
42	25.013859550185906	22.044331443973963	27.28401802697355	25.657790978866572
43	23.318693187792125	20.993813383546325	27.563957044952737	28.123536383708814
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	91.0
1	87.5
2	84.0
3	255.5
4	427.0
5	427.0
6	574.0
7	721.0
8	625.5
9	530.0
10	810.0
11	1090.0
12	1090.0
13	1843.0
14	2596.0
15	4116.0
16	5636.0
17	5423.5
18	5211.0
19	5211.0
20	6117.0
21	7023.0
22	6655.5
23	6288.0
24	7617.5
25	8947.0
26	8947.0
27	10682.0
28	12417.0
29	15920.5
30	19424.0
31	22584.5
32	25745.0
33	25745.0
34	30786.5
35	35828.0
36	43689.0
37	51550.0
38	59272.5
39	66995.0
40	66995.0
41	73371.5
42	79748.0
43	82818.5
44	85889.0
45	93281.0
46	100673.0
47	100673.0
48	107860.0
49	115047.0
50	118547.0
51	122047.0
52	129629.5
53	137212.0
54	137212.0
55	133157.0
56	129102.0
57	126734.0
58	124366.0
59	119834.5
60	115303.0
61	115303.0
62	108723.5
63	102144.0
64	95074.5
65	88005.0
66	78966.5
67	69928.0
68	69928.0
69	60281.0
70	50634.0
71	44702.0
72	38770.0
73	30315.5
74	21861.0
75	21861.0
76	17413.0
77	12965.0
78	11165.5
79	9366.0
80	8276.5
81	7187.0
82	7187.0
83	6003.5
84	4820.0
85	4289.5
86	3759.0
87	3238.5
88	2718.0
89	2718.0
90	2062.0
91	1406.0
92	834.0
93	262.0
94	177.5
95	93.0
96	93.0
97	55.5
98	18.0
99	14.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1673936.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.199840444828524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.95090595585603	44.15121445962092
2	6.845790272852678	6.873151588319272
3	1.7957452673101844	2.704383776955816
4	0.845171871913843	1.697099724741279
5	0.4755850759806831	1.1937147466085973
6	0.3117413726189249	0.938962029931312
7	0.21580044763372508	0.75832036273949
8	0.17452481183656637	0.7008894166287482
9	0.1321474203569146	0.5970401475401519
>10	0.9567598466493712	9.72818018343837
>50	0.14357747380186087	5.060298731776499
>100	0.126457320733209	13.199597503029507
>500	0.016956418836891363	5.728280404464538
>1k	0.008836443619225075	6.668866924205571
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3165	0.18907532904483804	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2873	0.1716314124315386	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2667	0.1593250876974986	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2567	0.1533511436518481	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2549	0.15227583372363102	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2467	0.1473771996061976	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2454	0.14660058688026303	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	2390	0.14277726269104674	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	2218	0.13250207893252788	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2095	0.1251541277563778	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	2066	0.12342168398313914	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1976	0.11804513434205369	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	1890	0.11290754246279429	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	1822	0.10884526051175195	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	1768	0.10561933072710068	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	1765	0.10544011240573117	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	1741	0.10400636583477504	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	1724	0.10299079534701447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.9739440456504906E-5	0.0	0.0	0.0
3	0.0	5.9739440456504906E-5	0.0	0.0	0.0
4	0.0	5.9739440456504906E-5	0.0	0.0	0.0
5	0.0	1.1947888091300981E-4	0.0	0.0	5.9739440456504906E-5
6	0.0	1.1947888091300981E-4	0.0	0.0	5.9739440456504906E-5
7	0.0	1.1947888091300981E-4	0.0	0.0	5.9739440456504906E-5
8	0.0	1.1947888091300981E-4	0.0	0.0	1.1947888091300981E-4
9	0.0	1.1947888091300981E-4	0.0	0.0	1.1947888091300981E-4
10	0.0	1.1947888091300981E-4	0.0	1.1947888091300981E-4	1.1947888091300981E-4
11	0.0	1.1947888091300981E-4	0.0	2.9869720228252456E-4	1.1947888091300981E-4
12	0.0	1.1947888091300981E-4	0.0	4.1817608319553437E-4	1.1947888091300981E-4
13	0.0	1.1947888091300981E-4	0.0	5.973944045650491E-4	1.1947888091300981E-4
14	0.0	1.7921832136951472E-4	0.0	7.766127259345639E-4	1.1947888091300981E-4
15	0.0	1.7921832136951472E-4	0.0	0.0013740071304996129	1.1947888091300981E-4
16	0.0	2.3895776182601962E-4	0.0	0.0023895776182601964	1.7921832136951472E-4
17	0.0	2.3895776182601962E-4	0.0	0.0035843664273902945	2.3895776182601962E-4
18	0.0	2.3895776182601962E-4	0.0	0.004241500272411849	2.3895776182601962E-4
19	0.0	2.3895776182601962E-4	0.0	0.005734986283824471	2.3895776182601962E-4
20	0.0	2.3895776182601962E-4	0.0	0.0069895145334110745	2.3895776182601962E-4
21	0.0	2.3895776182601962E-4	0.0	0.009379092151671271	4.1817608319553437E-4
22	0.0	2.3895776182601962E-4	0.0	0.012425803614953022	4.1817608319553437E-4
23	5.9739440456504906E-5	2.3895776182601962E-4	0.0	0.01863870542242953	4.1817608319553437E-4
24	5.9739440456504906E-5	2.9869720228252456E-4	0.0	0.027719100371818278	4.1817608319553437E-4
25	5.9739440456504906E-5	2.9869720228252456E-4	0.0	0.03225929784651265	4.1817608319553437E-4
26	5.9739440456504906E-5	2.9869720228252456E-4	0.0	0.039248812379923725	4.7791552365203925E-4
27	5.9739440456504906E-5	2.9869720228252456E-4	0.0	0.06421989849074278	4.7791552365203925E-4
28	5.9739440456504906E-5	2.9869720228252456E-4	0.0	0.17443916613299434	5.376549641085441E-4
29	5.9739440456504906E-5	3.5843664273902943E-4	0.0	0.32868640139169003	5.376549641085441E-4
30	5.9739440456504906E-5	3.5843664273902943E-4	0.0	0.5522911270203879	5.376549641085441E-4
31	5.9739440456504906E-5	3.5843664273902943E-4	0.0	1.059299758174745	5.376549641085441E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1755	0.0	23.507122	1
GTCTATA	45	0.0038265272	20.555555	1
CGGGTAG	50	0.007036189	18.5	10
ACGAATA	50	0.007036189	18.5	3
TTAGGCA	95	3.6083784E-6	17.526316	4
ATTACAC	90	4.447908E-5	16.444445	3
GTAATAC	90	4.447908E-5	16.444445	3
TATACAG	90	4.447908E-5	16.444445	5
CGATTAA	115	1.2429482E-6	16.086956	24
GCATTAT	185	1.8189894E-11	16.0	1
GCTAGAT	105	9.348112E-6	15.857144	3
ACTAGAC	70	0.002593157	15.857142	3
GTATCAA	2620	0.0	15.675573	2
TCTAGCG	650	0.0	15.653846	28
CTAGCGG	655	0.0	15.534351	29
CGCAAGA	790	0.0	15.455696	2
ACGATTA	120	1.9366853E-6	15.416667	23
CTTATAC	1795	0.0	15.356546	37
CGAACGA	535	0.0	15.214954	16
ACGAACG	525	0.0	15.152382	15
>>END_MODULE