FastQCFastQC Report
Fri 10 Feb 2017
ERR1631239.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631239.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73788
Sequences flagged as poor quality0
Sequence length43
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1570.21277172439963138No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1350.1829565783054155No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1340.18160134439204206No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC1000.1355233913373448No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC980.13281292351059792No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT870.11790535046349No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT810.10977394698324931No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC780.10570824524312897No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC760.10299777741638205No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCCG250.005463466529.621
GGACAAC400.001914194723.1250026
AGGGACG400.001914194723.1250028
CTTATAC650.001561062817.07692337
ATGTCCG803.329199E-416.18757
CCTTAGA750.004056185514.7999991
TTAGATG750.004056185514.7999993
GGCCCGG750.004056185514.79999916
TAGATGT750.004056185514.7999994
CTTAGAT800.00622523213.8750012
TCTTATA1102.4078666E-413.45454537
GCACGCG850.00929606113.05882319
GCTACAC850.00929606113.05882327
ACGCGCG850.00929606113.05882321
CTCTTAT1705.1213283E-611.97058837
CGCGCGC1150.00514020811.2608722
TCTCTTA2351.3014869E-711.02127737
GTCTCTT3105.881102E-78.95161337
GCCTGTC1700.0090780958.70588235
TGTCTCT4251.1505108E-88.27058837
Produced by FastQC (version 0.11.5)