##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631239.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	73788
Sequences flagged as poor quality	0
Sequence length	43
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84267089499647	34.0	31.0	34.0	31.0	34.0
2	33.022700168049006	34.0	33.0	34.0	31.0	34.0
3	33.144576353878676	34.0	33.0	34.0	31.0	34.0
4	36.46889738168808	37.0	37.0	37.0	35.0	37.0
5	36.440694963950776	37.0	37.0	37.0	35.0	37.0
6	36.4752127717244	37.0	37.0	37.0	35.0	37.0
7	36.45137420718816	37.0	37.0	37.0	35.0	37.0
8	36.43078820404402	37.0	37.0	37.0	35.0	37.0
9	38.31032146148425	39.0	39.0	39.0	37.0	39.0
10	38.24418604651163	39.0	39.0	39.0	37.0	39.0
11	38.3243616848268	39.0	39.0	39.0	37.0	39.0
12	38.072084891852334	39.0	39.0	39.0	37.0	39.0
13	38.20080500894454	39.0	39.0	39.0	37.0	39.0
14	39.72636472055077	41.0	40.0	41.0	38.0	41.0
15	39.75986610288936	41.0	40.0	41.0	38.0	41.0
16	39.74653060118177	41.0	40.0	41.0	38.0	41.0
17	39.714492871469616	41.0	40.0	41.0	38.0	41.0
18	39.68229251368786	41.0	40.0	41.0	37.0	41.0
19	39.69556025369979	41.0	40.0	41.0	37.0	41.0
20	39.64712419363582	41.0	40.0	41.0	37.0	41.0
21	39.60402775519054	41.0	40.0	41.0	37.0	41.0
22	39.56623028134656	41.0	39.0	41.0	37.0	41.0
23	39.490825066406465	41.0	39.0	41.0	37.0	41.0
24	39.469303951862095	41.0	39.0	41.0	37.0	41.0
25	39.42380874939015	41.0	39.0	41.0	37.0	41.0
26	39.349569035615545	41.0	39.0	41.0	36.0	41.0
27	39.27360817477096	41.0	39.0	41.0	36.0	41.0
28	39.172250230389764	41.0	39.0	41.0	36.0	41.0
29	39.10217108472922	41.0	39.0	41.0	35.0	41.0
30	39.02484143763213	40.0	39.0	41.0	35.0	41.0
31	38.983113785439365	40.0	39.0	41.0	35.0	41.0
32	38.915243671057624	40.0	39.0	41.0	35.0	41.0
33	38.79641676153304	40.0	38.0	41.0	35.0	41.0
34	38.75746733886269	40.0	38.0	41.0	35.0	41.0
35	38.63443920420664	40.0	38.0	41.0	35.0	41.0
36	38.547799100124685	40.0	38.0	41.0	35.0	41.0
37	38.49375237165935	40.0	38.0	41.0	35.0	41.0
38	38.38232503930178	40.0	38.0	41.0	34.0	41.0
39	38.29643031387217	40.0	37.0	41.0	34.0	41.0
40	38.184420230931856	40.0	37.0	41.0	34.0	41.0
41	38.09744131837155	40.0	37.0	41.0	34.0	41.0
42	37.985038217596355	40.0	37.0	41.0	34.0	41.0
43	36.97324768255001	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	2.0
20	10.0
21	8.0
22	18.0
23	30.0
24	55.0
25	90.0
26	147.0
27	173.0
28	243.0
29	310.0
30	426.0
31	588.0
32	748.0
33	1081.0
34	1615.0
35	2415.0
36	4074.0
37	7005.0
38	16674.0
39	38074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.61972136390741	15.685477313384292	11.016696481812762	28.67810484089554
2	21.872120127934082	18.24009324009324	29.934406678592723	29.953379953379955
3	21.47774705914241	19.024773675936466	26.462297392529948	33.03518187239117
4	16.154388247411504	14.028026237328564	30.457526969154873	39.36005854610506
5	18.588388355830215	32.78310836450372	31.028080446685095	17.60042283298097
6	39.88317883666721	31.266601615438827	13.49270884154605	15.357510706347915
7	33.91608391608392	26.516506749064888	18.477259174933593	21.090150159917602
8	31.372309860681952	29.113134927088414	17.519108798178564	21.995446414051063
9	28.34065159646555	12.718870277009811	16.208597603946444	42.731880522578194
10	19.66308884913536	22.827560036862362	28.767550279178185	28.741800834824087
11	42.098986285032794	18.442023093185885	17.704775844310728	21.75421477747059
12	25.90665148804684	21.273106738223017	24.905133626063858	27.915108147666288
13	34.96232449720822	15.44560091071719	21.04542744077628	28.546647151298316
14	25.692524529733834	16.940423917168104	20.18891960752426	37.1781319455738
15	29.697240743752374	23.23141974304765	18.13845069659023	28.932888816609747
16	30.477855477855474	21.233804954735184	19.57364341085271	28.714696156556624
17	28.789234021792158	22.051011004499376	20.363744782349432	28.796010191359027
18	29.435680598471297	20.11844744402884	21.485878462622647	28.959993494877217
19	29.8029489889955	20.862470862470865	20.14555212229631	29.18902802623733
20	29.290670569740335	20.183498671870765	20.638857266764244	29.88697349162465
21	30.922372201441966	20.867891798124354	20.14961782403643	28.060118176397246
22	30.644549249200416	20.134710250989322	20.108960806635228	29.111779693175045
23	29.199869897544318	20.053396216186915	20.69984279286605	30.04689109340272
24	28.748577004390956	21.079308288610616	20.378652355396543	29.793462351601885
25	29.483113785439368	20.117092210115466	20.62666016154388	29.773133842901284
26	29.63083428199707	21.36390741041904	20.291917384940643	28.713340922643248
27	30.03062828644224	20.25668130319293	20.527728085867622	29.18496232449721
28	28.943730687916734	20.628015395457254	20.71610559982653	29.71214831679948
29	27.742993440667856	21.141649048625794	21.803003198352037	29.312354312354312
30	27.61018051715726	21.95343416273649	21.503496503496503	28.932888816609747
31	29.7162140185396	21.110478668618203	20.141486420556188	29.03182089228601
32	28.2512061581829	21.075242586870495	20.847563289423753	29.82598796552285
33	27.653548002385214	20.56296416761533	21.63224372526698	30.151244104732477
34	28.899007968775408	20.488426302379793	21.69458448528216	28.91798124356264
35	28.67810484089554	20.717460833739903	22.267848430639127	28.33658589472543
36	27.452973383205943	21.77725375399794	21.904645741855045	28.865127120941075
37	28.52902911042446	19.793191304819214	22.557868488101047	29.119911096655283
38	27.054534612674146	20.02764677183282	23.845340705805825	29.072477909687212
39	27.373014582316905	19.27684718382393	24.12045319022063	29.229685043638533
40	26.809237274353553	19.579064346506208	24.7601235973329	28.851574781807336
41	25.237165934840355	19.724074375237166	26.189895375941884	28.84886431398059
42	25.788746137583345	19.47471133517645	26.432482246435736	28.304060280804467
43	24.08792757629967	18.675123326286116	26.990838618745595	30.24611047866862
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	1.0
7	1.0
8	1.5
9	2.0
10	2.5
11	3.0
12	3.0
13	5.5
14	8.0
15	10.0
16	12.0
17	11.5
18	11.0
19	11.0
20	21.5
21	32.0
22	25.0
23	18.0
24	26.5
25	35.0
26	35.0
27	63.0
28	91.0
29	148.0
30	205.0
31	249.5
32	294.0
33	294.0
34	448.5
35	603.0
36	808.0
37	1013.0
38	1323.0
39	1633.0
40	1633.0
41	1878.5
42	2124.0
43	2459.0
44	2794.0
45	3173.5
46	3553.0
47	3553.0
48	4013.0
49	4473.0
50	4922.0
51	5371.0
52	5750.0
53	6129.0
54	6129.0
55	6262.0
56	6395.0
57	6462.0
58	6529.0
59	6615.0
60	6701.0
61	6701.0
62	6500.5
63	6300.0
64	5927.5
65	5555.0
66	5050.0
67	4545.0
68	4545.0
69	3858.0
70	3171.0
71	2738.5
72	2306.0
73	1807.0
74	1308.0
75	1308.0
76	1116.0
77	924.0
78	777.5
79	631.0
80	552.0
81	473.0
82	473.0
83	369.5
84	266.0
85	194.0
86	122.0
87	105.5
88	89.0
89	89.0
90	68.5
91	48.0
92	29.5
93	11.0
94	8.5
95	6.0
96	6.0
97	3.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	73788.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.28102130427712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.9348014265434	72.75031170380008
2	3.8381634984938193	6.0091071718978695
3	1.0612513417125446	2.4922751666937715
4	0.5245663238807521	1.6425435030086193
5	0.3116235587410408	1.2197105220361035
6	0.1869741352446245	0.8781915758659945
7	0.19909282919566498	1.0909633002656258
8	0.1367681174474568	0.8565078332520192
9	0.08483085765728333	0.5976581557976907
>10	0.6751843772722551	9.769881281509187
>50	0.041549807832138776	2.1155201387759526
>100	0.005193725979017347	0.577329647097089
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	157	0.21277172439963138	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	135	0.1829565783054155	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	134	0.18160134439204206	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	100	0.1355233913373448	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	98	0.13281292351059792	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	87	0.11790535046349	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	81	0.10977394698324931	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	78	0.10570824524312897	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	76	0.10299777741638205	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0013552339133734482	0.0
22	0.0	0.0	0.0	0.0027104678267468965	0.0
23	0.0	0.0	0.0	0.0027104678267468965	0.0
24	0.0	0.0	0.0	0.004065701740120345	0.0
25	0.0	0.0	0.0	0.004065701740120345	0.0
26	0.0	0.0	0.0	0.004065701740120345	0.0
27	0.0	0.0	0.0	0.02303897652734862	0.0
28	0.0	0.0	0.0	0.11383964872336966	0.0
29	0.0	0.0	0.0	0.24123163658047378	0.0
30	0.0	0.0	0.0	0.39708353661842033	0.0
31	0.0	0.0	0.0	0.7575757575757576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATGCCG	25	0.0054634665	29.6	21
GGACAAC	40	0.0019141947	23.125002	6
AGGGACG	40	0.0019141947	23.125002	8
CTTATAC	65	0.0015610628	17.076923	37
ATGTCCG	80	3.329199E-4	16.1875	7
CCTTAGA	75	0.0040561855	14.799999	1
TTAGATG	75	0.0040561855	14.799999	3
GGCCCGG	75	0.0040561855	14.799999	16
TAGATGT	75	0.0040561855	14.799999	4
CTTAGAT	80	0.006225232	13.875001	2
TCTTATA	110	2.4078666E-4	13.454545	37
GCACGCG	85	0.009296061	13.058823	19
GCTACAC	85	0.009296061	13.058823	27
ACGCGCG	85	0.009296061	13.058823	21
CTCTTAT	170	5.1213283E-6	11.970588	37
CGCGCGC	115	0.005140208	11.26087	22
TCTCTTA	235	1.3014869E-7	11.021277	37
GTCTCTT	310	5.881102E-7	8.951613	37
GCCTGTC	170	0.009078095	8.705882	35
TGTCTCT	425	1.1505108E-8	8.270588	37
>>END_MODULE