FastQCFastQC Report
Fri 10 Feb 2017
ERR1631238.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631238.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70000
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11811.6871428571428573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10091.4414285714285715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7141.02No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.5328571428571428No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2720.38857142857142857No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1740.24857142857142855No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.21714285714285717No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.18571428571428572No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.16571428571428573No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1090.15571428571428572No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1040.14857142857142858No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1000.14285714285714285No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.13714285714285715No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.13428571428571429No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC870.12428571428571428No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA840.12No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT800.1142857142857143No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA770.11No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA750.10714285714285715No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT750.10714285714285715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGAC250.00546158829.630
ATTGACT250.00546158829.631
ATGCCCC358.786114E-426.4285737
GGGCAGT1900.023.3684227
TGGGCAG1900.023.3684226
GGCAGTC2000.023.1250028
CAGTCGG2050.022.56097410
GCAGTCG2050.022.5609749
CGCTTAT450.003790562720.55555726
GCTTATT450.003790562720.55555727
AGTCGGT2250.020.55555511
GGTATCA5500.019.8454531
TGATTCC2300.019.30434817
ATTCCTC2300.019.30434819
GGTGATT2350.018.89361815
GTGATTC2350.018.89361816
GATTCCT2350.018.89361818
TCGGTGA2550.018.86274313
CTCTATG3450.018.7681161
CGCCTTA2000.018.525
Produced by FastQC (version 0.11.5)