##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631238.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70000
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77582857142857	34.0	31.0	34.0	31.0	34.0
2	32.917185714285715	34.0	33.0	34.0	31.0	34.0
3	32.9803	34.0	33.0	34.0	31.0	34.0
4	36.34491428571429	37.0	37.0	37.0	35.0	37.0
5	36.345642857142856	37.0	37.0	37.0	35.0	37.0
6	36.40357142857143	37.0	37.0	37.0	35.0	37.0
7	36.3937	37.0	37.0	37.0	35.0	37.0
8	36.40495714285714	37.0	37.0	37.0	35.0	37.0
9	38.21555714285714	39.0	39.0	39.0	37.0	39.0
10	38.183014285714286	39.0	39.0	39.0	37.0	39.0
11	38.25194285714286	39.0	39.0	39.0	37.0	39.0
12	38.04457142857143	39.0	39.0	39.0	37.0	39.0
13	38.1662	39.0	39.0	39.0	37.0	39.0
14	39.60648571428571	41.0	39.0	41.0	37.0	41.0
15	39.60145714285714	41.0	39.0	41.0	37.0	41.0
16	39.52215714285714	41.0	39.0	41.0	37.0	41.0
17	39.54125714285714	41.0	39.0	41.0	37.0	41.0
18	39.59167142857143	41.0	39.0	41.0	37.0	41.0
19	39.5924	41.0	40.0	41.0	37.0	41.0
20	39.575042857142854	41.0	40.0	41.0	37.0	41.0
21	39.56695714285714	41.0	40.0	41.0	37.0	41.0
22	39.45727142857143	41.0	39.0	41.0	37.0	41.0
23	39.384457142857144	41.0	39.0	41.0	36.0	41.0
24	39.33365714285714	41.0	39.0	41.0	36.0	41.0
25	39.308814285714284	41.0	39.0	41.0	36.0	41.0
26	39.11847142857143	41.0	39.0	41.0	35.0	41.0
27	38.96375714285714	41.0	39.0	41.0	35.0	41.0
28	38.83775714285714	40.0	38.0	41.0	35.0	41.0
29	38.668142857142854	40.0	38.0	41.0	35.0	41.0
30	38.478942857142854	40.0	37.0	41.0	35.0	41.0
31	38.26382857142857	40.0	37.0	41.0	35.0	41.0
32	38.063942857142855	40.0	36.0	41.0	35.0	41.0
33	37.855514285714285	40.0	35.0	41.0	34.0	41.0
34	37.65981428571428	40.0	35.0	41.0	34.0	41.0
35	37.46154285714286	40.0	35.0	41.0	33.0	41.0
36	37.28718571428571	40.0	35.0	41.0	33.0	41.0
37	37.06514285714286	39.0	35.0	41.0	33.0	41.0
38	36.836214285714284	39.0	35.0	41.0	33.0	41.0
39	36.53051428571428	39.0	35.0	41.0	32.0	41.0
40	36.24388571428572	39.0	35.0	41.0	31.0	41.0
41	35.930571428571426	38.0	35.0	41.0	31.0	41.0
42	35.610342857142854	38.0	35.0	41.0	29.0	41.0
43	34.45344285714286	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	3.0
19	4.0
20	9.0
21	9.0
22	35.0
23	41.0
24	87.0
25	140.0
26	209.0
27	311.0
28	393.0
29	646.0
30	779.0
31	952.0
32	1136.0
33	1488.0
34	2098.0
35	2926.0
36	4866.0
37	11100.0
38	15950.0
39	26815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.82	15.685714285714287	12.702857142857143	24.791428571428572
2	21.417142857142856	19.697142857142858	30.674285714285716	28.21142857142857
3	23.132857142857144	20.96	26.994285714285716	28.912857142857142
4	16.185714285714287	16.025714285714283	28.660000000000004	39.128571428571426
5	17.161428571428573	35.792857142857144	27.57857142857143	19.467142857142857
6	33.77142857142857	28.778571428571432	17.481428571428573	19.968571428571426
7	31.97142857142857	29.677142857142858	16.647142857142857	21.704285714285714
8	34.39714285714286	27.55	16.657142857142855	21.395714285714284
9	30.69142857142857	11.881428571428572	17.44142857142857	39.98571428571429
10	22.935714285714287	24.225714285714286	23.64	29.19857142857143
11	37.792857142857144	22.33857142857143	16.06142857142857	23.807142857142857
12	24.404285714285713	26.479999999999997	23.292857142857144	25.822857142857142
13	36.01	16.939999999999998	22.78	24.27
14	25.56	19.344285714285714	22.838571428571427	32.25714285714286
15	32.84571428571429	22.142857142857142	19.305714285714284	25.705714285714286
16	26.96428571428571	22.838571428571427	23.344285714285714	26.852857142857147
17	24.064285714285713	26.25	23.61	26.075714285714284
18	25.06	20.91857142857143	26.071428571428573	27.950000000000003
19	25.56857142857143	24.895714285714284	26.75142857142857	22.784285714285716
20	26.608571428571427	21.364285714285714	27.71142857142857	24.315714285714286
21	28.83857142857143	20.388571428571428	25.947142857142858	24.825714285714287
22	28.274285714285714	20.998571428571427	24.472857142857144	26.254285714285714
23	25.80428571428571	22.457142857142856	25.10142857142857	26.63714285714286
24	25.324285714285715	22.498571428571427	26.478571428571428	25.698571428571427
25	25.202857142857145	22.64142857142857	25.13142857142857	27.02428571428571
26	26.477142857142855	21.834285714285713	25.567142857142855	26.121428571428574
27	25.822857142857142	21.675714285714285	25.00428571428571	27.497142857142858
28	25.03142857142857	22.675714285714285	25.788571428571426	26.504285714285714
29	24.38285714285714	22.99142857142857	27.36142857142857	25.264285714285716
30	24.291428571428572	23.325714285714287	28.050000000000004	24.332857142857144
31	24.484285714285715	24.572857142857142	25.985714285714284	24.95714285714286
32	23.65	24.54	27.82857142857143	23.98142857142857
33	24.318571428571428	23.142857142857142	27.44285714285714	25.095714285714287
34	25.040000000000003	23.322857142857142	25.525714285714287	26.11142857142857
35	23.875714285714285	24.33142857142857	26.17857142857143	25.61428571428571
36	22.835714285714285	23.787142857142857	27.11	26.267142857142854
37	22.958571428571428	23.84285714285714	27.171428571428574	26.02714285714286
38	22.925714285714285	22.040000000000003	29.03857142857143	25.995714285714282
39	23.502857142857142	21.64428571428571	29.31285714285714	25.540000000000003
40	22.84857142857143	21.51142857142857	28.35142857142857	27.28857142857143
41	20.73857142857143	21.858571428571427	30.345714285714287	27.057142857142857
42	20.821428571428573	21.65142857142857	29.722857142857144	27.804285714285715
43	19.931428571428572	23.041428571428572	30.534285714285716	26.49285714285714
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	11.5
2	16.0
3	49.5
4	83.0
5	83.0
6	126.0
7	169.0
8	174.5
9	180.0
10	304.5
11	429.0
12	429.0
13	822.5
14	1216.0
15	1761.0
16	2306.0
17	1962.5
18	1619.0
19	1619.0
20	1807.5
21	1996.0
22	1375.5
23	755.0
24	660.0
25	565.0
26	565.0
27	543.5
28	522.0
29	521.5
30	521.0
31	575.0
32	629.0
33	629.0
34	604.5
35	580.0
36	591.0
37	602.0
38	771.0
39	940.0
40	940.0
41	1081.5
42	1223.0
43	1658.5
44	2094.0
45	2570.5
46	3047.0
47	3047.0
48	4069.0
49	5091.0
50	5158.5
51	5226.0
52	4665.5
53	4105.0
54	4105.0
55	3986.5
56	3868.0
57	4060.5
58	4253.0
59	4326.0
60	4399.0
61	4399.0
62	4453.5
63	4508.0
64	4458.0
65	4408.0
66	4268.0
67	4128.0
68	4128.0
69	3838.0
70	3548.0
71	3129.5
72	2711.0
73	2319.5
74	1928.0
75	1928.0
76	1538.0
77	1148.0
78	908.0
79	668.0
80	494.5
81	321.0
82	321.0
83	224.5
84	128.0
85	87.0
86	46.0
87	28.5
88	11.0
89	11.0
90	8.0
91	5.0
92	3.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	70000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.84857142857143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.1628062609213	71.5942857142857
2	4.284864483027847	6.585714285714285
3	0.8848570472543406	2.04
4	0.4480053537569246	1.3771428571428572
5	0.22121426181358514	0.8500000000000001
6	0.1580101870097037	0.7285714285714286
7	0.1282670929843477	0.69
8	0.09108822545265272	0.5599999999999999
9	0.08179350856972896	0.5657142857142857
>10	0.48146633453545007	6.751428571428572
>50	0.037178867531694984	2.065714285714286
>100	0.014871547012677993	2.0428571428571427
>500	0.0018589433765847491	1.02
>1k	0.0037178867531694982	3.1285714285714286
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1181	1.6871428571428573	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1009	1.4414285714285715	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	714	1.02	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	373	0.5328571428571428	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	272	0.38857142857142857	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	174	0.24857142857142855	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	152	0.21714285714285717	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	130	0.18571428571428572	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	116	0.16571428571428573	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	109	0.15571428571428572	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	104	0.14857142857142858	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	100	0.14285714285714285	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	96	0.13714285714285715	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	94	0.13428571428571429	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	87	0.12428571428571428	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	84	0.12	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	80	0.1142857142857143	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	77	0.11	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	75	0.10714285714285715	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	75	0.10714285714285715	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0014285714285714286	0.0	0.0
5	0.0	0.0	0.0014285714285714286	0.0	0.0
6	0.0	0.0	0.0014285714285714286	0.0	0.0
7	0.0	0.0	0.0014285714285714286	0.0	0.0
8	0.0	0.0	0.0014285714285714286	0.0	0.0
9	0.0	0.0	0.0014285714285714286	0.0	0.0
10	0.0	0.0	0.0014285714285714286	0.0	0.0
11	0.0	0.0	0.002857142857142857	0.0	0.0
12	0.0	0.0	0.002857142857142857	0.0	0.0
13	0.0	0.0	0.002857142857142857	0.0	0.0
14	0.0	0.0	0.002857142857142857	0.0	0.0
15	0.0	0.0	0.002857142857142857	0.0	0.0
16	0.0	0.0	0.002857142857142857	0.0	0.0
17	0.0	0.0	0.002857142857142857	0.0014285714285714286	0.0
18	0.0	0.0	0.002857142857142857	0.0014285714285714286	0.0
19	0.0	0.0	0.002857142857142857	0.0014285714285714286	0.0
20	0.0	0.0	0.002857142857142857	0.0014285714285714286	0.0
21	0.0	0.0	0.002857142857142857	0.0014285714285714286	0.0
22	0.0	0.0	0.002857142857142857	0.002857142857142857	0.0
23	0.0	0.0	0.002857142857142857	0.008571428571428572	0.0
24	0.0	0.0	0.002857142857142857	0.022857142857142857	0.0014285714285714286
25	0.0	0.0	0.002857142857142857	0.027142857142857142	0.0014285714285714286
26	0.0	0.0	0.002857142857142857	0.041428571428571426	0.0014285714285714286
27	0.0	0.0	0.002857142857142857	0.10857142857142857	0.0014285714285714286
28	0.0	0.0	0.002857142857142857	0.31142857142857144	0.0014285714285714286
29	0.0	0.0	0.002857142857142857	0.5871428571428572	0.0014285714285714286
30	0.0	0.0	0.002857142857142857	0.9085714285714286	0.0014285714285714286
31	0.0	0.0	0.002857142857142857	1.5385714285714285	0.0014285714285714286
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTGAC	25	0.005461588	29.6	30
ATTGACT	25	0.005461588	29.6	31
ATGCCCC	35	8.786114E-4	26.42857	37
GGGCAGT	190	0.0	23.368422	7
TGGGCAG	190	0.0	23.368422	6
GGCAGTC	200	0.0	23.125002	8
CAGTCGG	205	0.0	22.560974	10
GCAGTCG	205	0.0	22.560974	9
CGCTTAT	45	0.0037905627	20.555557	26
GCTTATT	45	0.0037905627	20.555557	27
AGTCGGT	225	0.0	20.555555	11
GGTATCA	550	0.0	19.845453	1
TGATTCC	230	0.0	19.304348	17
ATTCCTC	230	0.0	19.304348	19
GGTGATT	235	0.0	18.893618	15
GTGATTC	235	0.0	18.893618	16
GATTCCT	235	0.0	18.893618	18
TCGGTGA	255	0.0	18.862743	13
CTCTATG	345	0.0	18.768116	1
CGCCTTA	200	0.0	18.5	25
>>END_MODULE