##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631237.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6422
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77125506072875	34.0	31.0	34.0	31.0	34.0
2	32.9630956088446	34.0	33.0	34.0	31.0	34.0
3	33.094674556213015	34.0	33.0	34.0	31.0	34.0
4	36.433821239489255	37.0	37.0	37.0	35.0	37.0
5	36.414356898162566	37.0	37.0	37.0	35.0	37.0
6	36.452818436624106	37.0	37.0	37.0	35.0	37.0
7	36.454219869199626	37.0	37.0	37.0	35.0	37.0
8	36.36359389598256	37.0	37.0	37.0	35.0	37.0
9	38.22936779819371	39.0	39.0	39.0	37.0	39.0
10	38.19635627530364	39.0	39.0	39.0	37.0	39.0
11	38.27810650887574	39.0	39.0	39.0	37.0	39.0
12	38.07941451261289	39.0	39.0	39.0	37.0	39.0
13	38.201183431952664	39.0	39.0	39.0	37.0	39.0
14	39.70414201183432	41.0	40.0	41.0	37.0	41.0
15	39.71332918094052	41.0	40.0	41.0	37.0	41.0
16	39.704764870756776	41.0	40.0	41.0	37.0	41.0
17	39.660074743070695	41.0	40.0	41.0	37.0	41.0
18	39.64559327312364	41.0	40.0	41.0	37.0	41.0
19	39.62830893802554	41.0	40.0	41.0	37.0	41.0
20	39.646060417315475	41.0	40.0	41.0	37.0	41.0
21	39.56026160074743	41.0	40.0	41.0	37.0	41.0
22	39.52974151354718	41.0	39.0	41.0	37.0	41.0
23	39.51308003737154	41.0	39.0	41.0	37.0	41.0
24	39.47430706944877	41.0	39.0	41.0	37.0	41.0
25	39.43304266583619	41.0	39.0	41.0	37.0	41.0
26	39.36452818436624	41.0	39.0	41.0	36.0	41.0
27	39.28931796947991	41.0	39.0	41.0	36.0	41.0
28	39.22858922454064	41.0	39.0	41.0	36.0	41.0
29	39.19775770787916	41.0	39.0	41.0	36.0	41.0
30	39.14029897228278	40.0	39.0	41.0	35.0	41.0
31	39.08455309872314	40.0	39.0	41.0	35.0	41.0
32	39.05574587355964	40.0	39.0	41.0	35.0	41.0
33	38.95982559950171	40.0	39.0	41.0	35.0	41.0
34	38.8122080348801	40.0	38.0	41.0	35.0	41.0
35	38.741202117720334	40.0	38.0	41.0	35.0	41.0
36	38.70834630956089	40.0	38.0	41.0	35.0	41.0
37	38.64123326066646	40.0	38.0	41.0	35.0	41.0
38	38.55777016505761	40.0	38.0	41.0	35.0	41.0
39	38.466054188726254	40.0	38.0	41.0	35.0	41.0
40	38.388663967611336	40.0	38.0	41.0	34.0	41.0
41	38.29679227654936	40.0	37.0	41.0	34.0	41.0
42	38.24649641856119	40.0	37.0	41.0	34.0	41.0
43	37.30348800996574	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	2.0
24	6.0
25	6.0
26	4.0
27	15.0
28	15.0
29	22.0
30	42.0
31	52.0
32	71.0
33	103.0
34	140.0
35	206.0
36	318.0
37	616.0
38	1459.0
39	3343.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91497975708502	17.75147928994083	11.538461538461538	27.795079414512614
2	20.009342883836812	18.452195577701648	32.964808470881344	28.573653067580196
3	21.17720336343818	20.9124883213952	27.608221737776397	30.30208657739022
4	15.602616007474307	14.76175646216132	32.71566490189972	36.91996262846465
5	17.611336032388664	33.77452507007163	31.033945811273746	17.58019308626596
6	37.823108066023046	31.376518218623485	15.32232949236998	15.478044222983495
7	32.62223606353161	27.748364995328558	18.296480847088134	21.3329180940517
8	29.89722827779508	30.084085954531297	18.919339769542198	21.099345998131422
9	28.573653067580196	13.235752102148862	17.78262223606353	40.407972594207415
10	19.947056991591406	23.69978199937714	29.212083463095606	27.141077545935843
11	38.88196823419495	20.834630956088446	18.794767985051386	21.488632824665213
12	25.568358766739337	23.341638118966053	24.696356275303643	26.393646838990968
13	31.983805668016196	17.39333540952974	22.127063220180627	28.495795702273437
14	24.18249766427904	18.779196511990033	21.784490812830896	35.25381501090003
15	28.791653690439116	24.805356586733105	18.84148240423544	27.561507318592337
16	26.907505450015574	24.634070383058237	20.803488009965744	27.654936156960446
17	27.87293677981937	23.450638430395514	21.83120523201495	26.845219557770168
18	27.1877919651199	21.706633447524133	23.51292432264092	27.59265026471504
19	27.49922142634693	22.51635004671442	22.469635627530366	27.514792899408285
20	27.545935845530988	21.706633447524133	21.597633136094675	29.1497975708502
21	29.6325132357521	23.123637496107133	20.850202429149796	26.393646838990968
22	29.32108377452507	22.454064154469012	21.519775770787916	26.705076300218
23	27.59265026471504	22.03363438181252	21.909062597321707	28.464652756150734
24	27.078791653690438	22.485207100591715	22.672064777327936	27.76393646838991
25	26.782933665524762	22.376206789162254	22.313920896916848	28.52693864839614
26	26.89193397695422	22.983494238554965	22.391778262223603	27.732793522267208
27	28.07536592961694	21.800062285892245	21.86234817813765	28.26222360635316
28	26.378075365929615	22.874493927125506	22.62535035814388	28.122080348801
29	24.898785425101213	22.983494238554965	24.75864216754905	27.35907816879477
30	26.30021800062286	23.09249454998443	24.05792587978823	26.549361569604486
31	27.561507318592337	23.668639053254438	21.955777016505763	26.814076611647465
32	25.895359701027715	22.95235129243226	22.967922765493615	28.184366241046405
33	24.78978511367175	22.640921831205233	23.357209592027406	29.212083463095606
34	28.65151043288695	21.286203674867643	23.41949548427281	26.642790407972594
35	26.066645904702586	21.784490812830896	23.839925256929305	28.308938025537216
36	24.525070071628775	22.858922454064153	25.45935845530987	27.156649018997197
37	26.44036125817502	20.3986297103706	25.41264403612582	27.748364995328558
38	24.27592650264715	20.616630333229523	26.175646216132048	28.931796947991277
39	24.711927748364996	19.99377141077546	27.483649953285582	27.810650887573964
40	24.680784802242293	20.538772967922768	28.184366241046405	26.596075988788538
41	22.313920896916848	20.274057925879788	29.912799750856433	27.49922142634693
42	23.403924011211462	20.08720024914357	29.663656181874803	26.845219557770168
43	22.53192151977577	20.040485829959515	30.457801308003734	26.96979134226098
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	1.0
9	0.0
10	0.5
11	1.0
12	1.0
13	1.5
14	2.0
15	7.0
16	12.0
17	14.0
18	16.0
19	16.0
20	20.0
21	24.0
22	21.5
23	19.0
24	21.5
25	24.0
26	24.0
27	33.5
28	43.0
29	54.5
30	66.0
31	76.5
32	87.0
33	87.0
34	102.0
35	117.0
36	147.5
37	178.0
38	193.5
39	209.0
40	209.0
41	234.5
42	260.0
43	287.5
44	315.0
45	345.5
46	376.0
47	376.0
48	406.0
49	436.0
50	457.5
51	479.0
52	506.0
53	533.0
54	533.0
55	499.5
56	466.0
57	487.5
58	509.0
59	500.5
60	492.0
61	492.0
62	481.0
63	470.0
64	428.5
65	387.0
66	346.5
67	306.0
68	306.0
69	253.5
70	201.0
71	191.5
72	182.0
73	125.5
74	69.0
75	69.0
76	59.0
77	49.0
78	37.5
79	26.0
80	28.0
81	30.0
82	30.0
83	21.5
84	13.0
85	10.0
86	7.0
87	8.0
88	9.0
89	9.0
90	6.5
91	4.0
92	2.5
93	1.0
94	1.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6422.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.29897228277795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.60234523193654	84.52195577701652
2	4.29384376616658	7.754593584553099
3	1.155371615795827	3.129866085331672
4	0.4138644593895499	1.494861413889754
5	0.18968787722021038	0.8564310183743382
6	0.17244352474564578	0.9342883836810962
7	0.08622176237282289	0.5450015571473061
8	0.05173305742369374	0.3737153534724385
9	0.01724435247456458	0.14014325755216445
>10	0.01724435247456458	0.24914356898162568
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	16	0.24914356898162568	No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG	9	0.14014325755216445	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	8	0.12457178449081284	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	8	0.12457178449081284	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.12457178449081284	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	7	0.10900031142946122	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.10900031142946122	No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCG	7	0.10900031142946122	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	7	0.10900031142946122	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT	7	0.10900031142946122	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.06228589224540641	0.0
28	0.0	0.0	0.0	0.15571473061351604	0.0
29	0.0	0.0	0.0	0.34257240734973526	0.0
30	0.0	0.0	0.0	0.5761445032700093	0.0
31	0.0	0.0	0.0	1.448146994705699	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTTAT	25	0.0051027173	29.6	37
TGTCTCT	85	0.008136338	13.058824	37
>>END_MODULE