FastQCFastQC Report
Fri 10 Feb 2017
ERR1631236.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631236.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57232
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA960.16773832820799553No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC850.1485183114341627No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC740.12929829466032988No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC660.11532010064299691No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC630.11007827788649706No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC600.1048364551299972No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA590.10308918087783057No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTG250.00545332929.5999988
GGTATCA751.3165845E-824.6666661
AATCTGT502.6588637E-422.19999936
TCTTATA1650.020.18181837
CTTATAC759.01607E-619.73333437
TGTTATT500.00695546118.49999822
GTATCAA1107.494018E-716.8181822
CTCTTAT2800.015.19642837
CAACGCA1501.868524E-512.3333336
ATCTGTC1204.991096E-412.33333337
TCAACGC1552.6154059E-511.9354845
TATCAAC1603.6191585E-511.56253
ATCAACG1603.6191585E-511.56254
ACTGTCT1451.8775158E-411.482758537
TCTCTTA3750.011.34666636
AACGCAG1706.714692E-510.8823537
CAGAGTA1706.714692E-510.88235311
ACGCAGA1759.013828E-510.5714288
AGAGTAC1759.013828E-510.57142812
CGCAGAG1759.013828E-510.5714289
Produced by FastQC (version 0.11.5)