##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631236.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	57232
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77184092815208	34.0	31.0	34.0	31.0	34.0
2	32.94756429969248	34.0	33.0	34.0	31.0	34.0
3	33.06796896840928	34.0	33.0	34.0	31.0	34.0
4	36.38941501258037	37.0	37.0	37.0	35.0	37.0
5	36.3798399496785	37.0	37.0	37.0	35.0	37.0
6	36.43442479731619	37.0	37.0	37.0	35.0	37.0
7	36.417895582890694	37.0	37.0	37.0	35.0	37.0
8	36.39135448700028	37.0	37.0	37.0	35.0	37.0
9	38.250751327928434	39.0	39.0	39.0	37.0	39.0
10	38.201303466592115	39.0	39.0	39.0	37.0	39.0
11	38.269551998881745	39.0	39.0	39.0	37.0	39.0
12	38.02341347497903	39.0	39.0	39.0	37.0	39.0
13	38.1581457925636	39.0	39.0	39.0	37.0	39.0
14	39.657883701425774	41.0	40.0	41.0	37.0	41.0
15	39.6885483645513	41.0	40.0	41.0	37.0	41.0
16	39.65224000559128	41.0	40.0	41.0	37.0	41.0
17	39.625786273413475	41.0	40.0	41.0	37.0	41.0
18	39.599507268660886	41.0	40.0	41.0	37.0	41.0
19	39.61649077439195	41.0	40.0	41.0	37.0	41.0
20	39.61345051719318	41.0	40.0	41.0	37.0	41.0
21	39.56583729382164	41.0	40.0	41.0	37.0	41.0
22	39.512650265585684	41.0	39.0	41.0	37.0	41.0
23	39.44036552977355	41.0	39.0	41.0	37.0	41.0
24	39.39535574503774	41.0	39.0	41.0	36.0	41.0
25	39.37347987140061	41.0	39.0	41.0	36.0	41.0
26	39.30837643276489	41.0	39.0	41.0	36.0	41.0
27	39.244897959183675	41.0	39.0	41.0	36.0	41.0
28	39.14142437797037	41.0	39.0	41.0	36.0	41.0
29	39.0606478892927	40.0	39.0	41.0	35.0	41.0
30	38.974647050601064	40.0	39.0	41.0	35.0	41.0
31	38.951181157394466	40.0	39.0	41.0	35.0	41.0
32	38.88406835336875	40.0	38.0	41.0	35.0	41.0
33	38.78742661448141	40.0	38.0	41.0	35.0	41.0
34	38.74846239865809	40.0	38.0	41.0	35.0	41.0
35	38.63834917528655	40.0	38.0	41.0	35.0	41.0
36	38.58425356443947	40.0	38.0	41.0	35.0	41.0
37	38.521264327648865	40.0	38.0	41.0	35.0	41.0
38	38.437098126922	40.0	38.0	41.0	34.0	41.0
39	38.353228962818	40.0	38.0	41.0	34.0	41.0
40	38.29245876432765	40.0	37.0	41.0	34.0	41.0
41	38.222864830863855	40.0	37.0	41.0	34.0	41.0
42	38.08558149287112	40.0	37.0	41.0	34.0	41.0
43	37.10350852669835	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	3.0
21	8.0
22	13.0
23	27.0
24	61.0
25	63.0
26	89.0
27	127.0
28	209.0
29	262.0
30	342.0
31	430.0
32	637.0
33	883.0
34	1348.0
35	1958.0
36	3042.0
37	5591.0
38	12707.0
39	29426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.393556052558004	17.268311434162705	12.258876153201006	27.07925636007828
2	21.227634889572265	18.475677942409842	31.323385518590996	28.97330164942689
3	20.775090858261112	19.88747553816047	27.76593514117976	31.571498462398655
4	15.41095890410959	14.720785574503775	32.27040816326531	37.597847358121335
5	17.816955549343025	33.666480290746435	31.463167458764328	17.053396701146212
6	37.40739446463517	32.57268660889013	14.439474419904949	15.580444506569751
7	32.930877830584286	27.62091137824993	19.150125803746157	20.298084987419625
8	30.390341067934024	30.0356443947442	18.06157394464635	21.51244059267543
9	28.134610008386918	13.322966172770478	17.263069611406205	41.2793542074364
10	19.679549902152644	23.385518590998043	28.94883980989656	27.98609169695275
11	40.833799273133906	19.574713447022646	18.10350852669835	21.487978753145093
12	24.713447022644676	23.50608051439754	24.91787811014817	26.862594352809616
13	33.58610567514677	16.06094492591557	22.42626502655857	27.92668437237909
14	25.311014816885656	18.182135868045847	21.094842046407607	35.412007268660886
15	29.378669275929546	24.29235392787252	18.603228962818	27.725747833379927
16	29.079885378809056	22.85434721833939	20.89565278166061	27.170114621190944
17	27.573734973441432	23.02383282079955	21.369164103997765	28.033268101761255
18	27.947651663405086	20.60036343304445	23.26845121610288	28.183533687447582
19	28.29885378809058	22.09952474140341	21.688915292144255	27.912706178361756
20	29.081632653061224	20.81178361755661	21.374405926754264	28.732177802627902
21	30.748532289628177	21.395373217780264	21.11755661168577	26.73853788090579
22	30.570310315907186	21.005731059547106	20.930598266703942	27.493360357841766
23	28.398448420464078	20.96204920324294	21.633002516074924	29.00649986021806
24	27.83407883701426	22.382583170254403	21.41284596030193	28.37049203242941
25	28.122379088621752	21.2573385518591	21.778026279004752	28.8422560805144
26	28.26216102879508	22.251537601341905	21.725608051439753	27.760693318423257
27	28.83002516074923	21.269569471624266	21.337713167458762	28.56269220016774
28	28.06471903830025	21.564858820240428	22.195624825272574	28.17479731618675
29	26.640690522784453	21.865389991613082	23.436189544310874	28.057729941291587
30	26.093793681856305	23.14963656695555	22.784456248252727	27.972113502935418
31	27.811364271736096	22.35986860497624	21.612035225048924	28.216731898238745
32	26.80493430248812	21.2835476656416	22.800181716522225	29.11133631534806
33	26.02040816326531	20.948071009225607	23.720995247414034	29.310525580095053
34	27.27844562482527	21.051160190103438	23.437936818563042	28.232457366508246
35	26.90977075761812	20.829256360078276	24.641808778305844	27.61916410399776
36	25.405367626502656	21.36042773273693	24.52823595191501	28.705968688845402
37	25.782778864970645	19.64984623986581	25.859658932065976	28.70771596309757
38	24.531730500419346	18.8076600503215	27.23476376852111	29.42584568073805
39	24.872448979591837	18.293961420184512	28.15907184791725	28.6745177523064
40	24.42165222253285	18.613712608331003	28.69199049482807	28.27264467430808
41	23.036063740564718	18.428501537601342	30.956457925636006	27.578976796197928
42	22.81241263628739	19.094213027676822	30.594772155437518	27.49860218059827
43	20.404668716801787	18.74650545149567	31.41424377970366	29.43458205199888
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	4.0
7	7.0
8	4.5
9	2.0
10	9.5
11	17.0
12	17.0
13	21.0
14	25.0
15	42.0
16	59.0
17	50.0
18	41.0
19	41.0
20	51.0
21	61.0
22	53.5
23	46.0
24	60.0
25	74.0
26	74.0
27	94.0
28	114.0
29	169.5
30	225.0
31	304.0
32	383.0
33	383.0
34	520.0
35	657.0
36	893.5
37	1130.0
38	1421.5
39	1713.0
40	1713.0
41	1986.0
42	2259.0
43	2562.0
44	2865.0
45	3141.5
46	3418.0
47	3418.0
48	3785.0
49	4152.0
50	4395.0
51	4638.0
52	4954.5
53	5271.0
54	5271.0
55	5155.0
56	5039.0
57	4943.0
58	4847.0
59	4795.5
60	4744.0
61	4744.0
62	4518.5
63	4293.0
64	3882.5
65	3472.0
66	3045.5
67	2619.0
68	2619.0
69	2237.5
70	1856.0
71	1571.0
72	1286.0
73	990.5
74	695.0
75	695.0
76	563.5
77	432.0
78	356.5
79	281.0
80	257.0
81	233.0
82	233.0
83	177.0
84	121.0
85	99.5
86	78.0
87	61.5
88	45.0
89	45.0
90	37.0
91	29.0
92	16.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	57232.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.75482247693598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.89860688255375	68.86007827788649
2	4.599132761324846	6.9681297176404815
3	1.492296337300489	3.39145932345541
4	0.8026570716855799	2.432205759015935
5	0.5051203985607529	1.913265306122449
6	0.38057016329919735	1.729801509644954
7	0.23526155549404926	1.2475538160469666
8	0.15684103699603286	0.9505171931786414
9	0.11532429190884769	0.7862734134749791
>10	0.7888181566565181	10.466172770478053
>50	0.02537134421994649	1.2545429130556331
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	96	0.16773832820799553	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	85	0.1485183114341627	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	74	0.12929829466032988	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	66	0.11532010064299691	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	63	0.11007827788649706	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGCTGTC	60	0.1048364551299972	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	59	0.10308918087783057	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.00174727425216662
2	0.0	0.0	0.0	0.0	0.00174727425216662
3	0.0	0.0	0.0	0.0	0.00174727425216662
4	0.0	0.0	0.0	0.0	0.00174727425216662
5	0.0	0.0	0.0	0.0	0.00174727425216662
6	0.0	0.0	0.0	0.0	0.00174727425216662
7	0.0	0.0	0.0	0.0	0.00174727425216662
8	0.0	0.0	0.0	0.0	0.00174727425216662
9	0.0	0.0	0.0	0.0	0.00174727425216662
10	0.0	0.0	0.0	0.0	0.00174727425216662
11	0.0	0.0	0.0	0.0	0.00174727425216662
12	0.0	0.0	0.0	0.0	0.00174727425216662
13	0.0	0.0	0.0	0.0	0.00174727425216662
14	0.0	0.0	0.0	0.0	0.00174727425216662
15	0.0	0.0	0.0	0.0	0.00174727425216662
16	0.0	0.0	0.0	0.0	0.00174727425216662
17	0.0	0.0	0.0	0.0	0.00174727425216662
18	0.0	0.0	0.00174727425216662	0.0	0.00174727425216662
19	0.0	0.0	0.00174727425216662	0.00174727425216662	0.00174727425216662
20	0.0	0.0	0.00174727425216662	0.00174727425216662	0.00174727425216662
21	0.0	0.0	0.00174727425216662	0.00174727425216662	0.00174727425216662
22	0.0	0.0	0.00174727425216662	0.00524182275649986	0.00174727425216662
23	0.0	0.0	0.00174727425216662	0.00524182275649986	0.00174727425216662
24	0.0	0.0	0.00174727425216662	0.00524182275649986	0.00174727425216662
25	0.0	0.0	0.00174727425216662	0.01223091976516634	0.00174727425216662
26	0.0	0.0	0.00174727425216662	0.02096729102599944	0.00174727425216662
27	0.0	0.0	0.00174727425216662	0.06814369583449818	0.00174727425216662
28	0.0	0.0	0.00174727425216662	0.2795638803466592	0.00174727425216662
29	0.0	0.00174727425216662	0.00174727425216662	0.5905786972323176	0.00174727425216662
30	0.0	0.00174727425216662	0.00174727425216662	1.0273972602739727	0.00174727425216662
31	0.0	0.00174727425216662	0.00174727425216662	2.1124545708694438	0.00174727425216662
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAGGTG	25	0.005453329	29.599998	8
GGTATCA	75	1.3165845E-8	24.666666	1
AATCTGT	50	2.6588637E-4	22.199999	36
TCTTATA	165	0.0	20.181818	37
CTTATAC	75	9.01607E-6	19.733334	37
TGTTATT	50	0.006955461	18.499998	22
GTATCAA	110	7.494018E-7	16.818182	2
CTCTTAT	280	0.0	15.196428	37
CAACGCA	150	1.868524E-5	12.333333	6
ATCTGTC	120	4.991096E-4	12.333333	37
TCAACGC	155	2.6154059E-5	11.935484	5
TATCAAC	160	3.6191585E-5	11.5625	3
ATCAACG	160	3.6191585E-5	11.5625	4
ACTGTCT	145	1.8775158E-4	11.4827585	37
TCTCTTA	375	0.0	11.346666	36
AACGCAG	170	6.714692E-5	10.882353	7
CAGAGTA	170	6.714692E-5	10.882353	11
ACGCAGA	175	9.013828E-5	10.571428	8
AGAGTAC	175	9.013828E-5	10.571428	12
CGCAGAG	175	9.013828E-5	10.571428	9
>>END_MODULE