FastQCFastQC Report
Fri 10 Feb 2017
ERR1631235.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631235.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310121
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT141700.6133877835836304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110600.47876280073641164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT99890.43240159281699964No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC60390.2614148782682812No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT47350.20496761857928653No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42130.18237139959335463No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA31750.13743868827650155No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC27310.11821891580570888No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT25600.11081670613790359No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC25220.10917177065616909No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG25100.10865231734614768No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC24700.10692080631274292No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT23340.1010336687991668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATCA17100.020.44736914
GACAGGC17900.020.2569857
GCATCAG17700.019.96327615
GCAGTCG19000.019.8631579
GCTGCAT17850.019.69187712
CAGGACA20300.019.5935964
CTGCATC18100.019.31767813
CAGTCGG19650.019.30025510
AGTCGGT19450.019.21336711
CCAGGAC20150.019.0967753
ATCAGAA18600.019.09677317
GGCAGTC20700.018.6787458
ACAGGCT19400.018.5953628
CATCAGA19450.018.54755816
TCCAGGA21350.018.3700242
GGGCAGT21300.018.2394377
TCGGTGA21300.017.63145413
GTGATTC20950.017.57279216
GGTATCA66650.017.3480871
CGGTGAT21700.017.22119914
Produced by FastQC (version 0.11.5)