##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631235.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2310121
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.534123970129706	34.0	31.0	34.0	31.0	34.0
2	32.72250284725346	34.0	31.0	34.0	31.0	34.0
3	32.814034849256814	34.0	31.0	34.0	31.0	34.0
4	36.274131095297605	37.0	37.0	37.0	35.0	37.0
5	36.19057486599187	37.0	35.0	37.0	35.0	37.0
6	36.225038428722996	37.0	37.0	37.0	35.0	37.0
7	36.21856907062444	37.0	37.0	37.0	35.0	37.0
8	36.20576281502138	37.0	37.0	37.0	35.0	37.0
9	37.99069615833976	39.0	38.0	39.0	35.0	39.0
10	37.91300498978192	39.0	38.0	39.0	35.0	39.0
11	37.982113491024926	39.0	38.0	39.0	35.0	39.0
12	37.7532921435717	39.0	38.0	39.0	35.0	39.0
13	37.88615617969795	39.0	38.0	39.0	35.0	39.0
14	39.286315305561914	40.0	39.0	41.0	36.0	41.0
15	39.26181832033907	40.0	39.0	41.0	36.0	41.0
16	39.23348257515516	40.0	39.0	41.0	36.0	41.0
17	39.230079290218995	40.0	39.0	41.0	36.0	41.0
18	39.18924246825166	40.0	39.0	41.0	36.0	41.0
19	39.207382210715366	40.0	39.0	41.0	36.0	41.0
20	39.188078459959456	40.0	39.0	41.0	36.0	41.0
21	39.16244084184335	40.0	39.0	41.0	36.0	41.0
22	39.09612310350843	40.0	39.0	41.0	36.0	41.0
23	39.02314077920594	40.0	39.0	41.0	35.0	41.0
24	38.978507619297865	40.0	38.0	41.0	35.0	41.0
25	38.95849481477377	40.0	38.0	41.0	35.0	41.0
26	38.84468908771445	40.0	38.0	41.0	35.0	41.0
27	38.77045877683463	40.0	38.0	41.0	35.0	41.0
28	38.65109533223584	40.0	38.0	41.0	35.0	41.0
29	38.55093477787527	40.0	38.0	41.0	35.0	41.0
30	38.4631601548144	40.0	38.0	41.0	35.0	41.0
31	38.39700517851662	40.0	38.0	41.0	35.0	41.0
32	38.29209552226918	40.0	38.0	41.0	34.0	41.0
33	38.16047124804285	40.0	37.0	41.0	34.0	41.0
34	38.092945780762136	40.0	37.0	41.0	34.0	41.0
35	37.94541281603864	40.0	37.0	41.0	33.0	41.0
36	37.872441313680106	40.0	37.0	41.0	33.0	41.0
37	37.77769865734306	40.0	37.0	41.0	33.0	41.0
38	37.66035458748698	40.0	36.0	41.0	33.0	41.0
39	37.543709182332876	40.0	36.0	41.0	33.0	41.0
40	37.39041721191228	40.0	36.0	41.0	33.0	41.0
41	37.233217221089284	40.0	36.0	41.0	33.0	41.0
42	37.08604570929401	40.0	35.0	41.0	32.0	41.0
43	36.087072062459065	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	6.0
12	6.0
13	9.0
14	7.0
15	16.0
16	28.0
17	43.0
18	89.0
19	161.0
20	313.0
21	589.0
22	941.0
23	1600.0
24	2552.0
25	3824.0
26	5720.0
27	8262.0
28	11418.0
29	15622.0
30	21125.0
31	27355.0
32	35269.0
33	48084.0
34	71991.0
35	104420.0
36	162424.0
37	312722.0
38	641882.0
39	833637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.914539541435275	16.94396094403713	12.370304412626005	26.77119510190159
2	20.249588657910127	19.254532554788256	33.223930694539376	27.271948092762237
3	21.075822435275036	21.43731865127411	28.050219014501838	29.436639898949018
4	15.264351953858693	15.194485483660813	31.75989482801983	37.78126773446066
5	16.548353960679982	35.192615451744736	30.955391514124152	17.30363907345113
6	35.55523714991553	32.19446080962859	15.887825789211906	16.362476251243983
7	31.487138552482747	28.856194112775913	19.33747193328834	20.319195401452998
8	30.175951822437007	30.50411645104304	18.439943189122996	20.879988537396958
9	28.814681135750032	13.407566097187118	18.184199009489113	39.59355375757374
10	20.290495606074312	24.602823834768827	28.19077442263847	26.91590613651839
11	37.879963863364736	21.22174552761522	18.732784992647574	22.165505616372474
12	25.001460962434436	24.73519785327262	25.3149943228082	24.948346861484744
13	31.792274084344502	17.316019377339977	23.91164791800949	26.980058620306036
14	24.304830785919872	19.576420455898198	23.070393282429794	33.04835547575213
15	28.378123916452864	24.84506222834215	20.480312503111307	26.296501352093678
16	26.894998140790026	23.826803877372658	22.14321241181739	27.134985570019925
17	25.351659068940545	25.003149185692003	23.40829766059873	26.236894084768718
18	25.692983181400454	21.771760007376237	25.39957863679002	27.135678174433288
19	26.23715381142373	23.73802064913483	24.501660302642154	25.523165236799283
20	26.4764919240161	22.409778535410048	24.73138852899913	26.382341011574717
21	29.432354409141336	22.28463357547072	23.670275279952868	24.61273673543507
22	28.37630582986779	22.476398422420296	23.177314088742538	25.969981658969377
23	26.301566021866385	22.885597767389672	23.818838926618994	26.993997284124944
24	26.05954406717224	23.5183351867716	24.382186041337228	26.03993470471893
25	25.902106426459916	22.531244034403393	24.33101989030012	27.235629648836575
26	26.850628170559034	23.50721022838198	24.5706177295475	25.071543871511494
27	27.564270442976795	22.27359519263277	23.45310050858808	26.70903385580236
28	25.335512728554043	22.580462235527925	25.489444059423725	26.594580976494303
29	25.22041053260847	22.8119219729183	25.88349268285081	26.084174811622425
30	24.431447530237595	23.61932556779493	26.18170217057894	25.76752473138853
31	25.671772171241248	23.64482206776182	24.26448657884154	26.418919182155392
32	24.69069801971412	23.05480968312915	25.315557063894055	26.938935233262672
33	25.154093660029066	22.84031875386614	25.466414962679444	26.539172623425355
34	26.213778412472767	23.14779182562299	24.853719783509177	25.78470997839507
35	26.144258244481566	22.89849752458854	25.53792636835906	25.419317862570836
36	24.422833262846407	23.675642964156424	24.976743642432584	26.924780130564592
37	24.59057339420749	22.574878112445194	26.03318181168865	26.801366681658667
38	24.604512058026398	21.414592568960675	27.184550073351136	26.796345299661795
39	24.15150548391188	21.554455372683943	27.39592428275402	26.898114860650157
40	24.041987411049032	21.33100387382306	28.138396213877975	26.488612501249936
41	22.252687196904404	21.74314678754922	29.4378519566724	26.566314058873974
42	22.916548527111782	22.177409754727133	28.585558938254753	26.320482779906335
43	21.703754911539267	20.97007039890984	29.638663948771516	27.68751074077938
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	302.0
1	282.0
2	262.0
3	879.0
4	1496.0
5	1496.0
6	2044.5
7	2593.0
8	2552.5
9	2512.0
10	3873.0
11	5234.0
12	5234.0
13	9069.0
14	12904.0
15	20144.0
16	27384.0
17	23997.0
18	20610.0
19	20610.0
20	22159.5
21	23709.0
22	17386.5
23	11064.0
24	11630.0
25	12196.0
26	12196.0
27	13538.5
28	14881.0
29	17960.0
30	21039.0
31	25583.0
32	30127.0
33	30127.0
34	38011.0
35	45895.0
36	55789.5
37	65684.0
38	75396.5
39	85109.0
40	85109.0
41	93009.5
42	100910.0
43	109206.0
44	117502.0
45	126655.0
46	135808.0
47	135808.0
48	149960.0
49	164112.0
50	169354.0
51	174596.0
52	178818.5
53	183041.0
54	183041.0
55	178484.5
56	173928.0
57	166258.0
58	158588.0
59	150153.0
60	141718.0
61	141718.0
62	136791.0
63	131864.0
64	122089.0
65	112314.0
66	99324.5
67	86335.0
68	86335.0
69	75241.0
70	64147.0
71	58080.0
72	52013.0
73	49184.5
74	46356.0
75	46356.0
76	42755.0
77	39154.0
78	31258.5
79	23363.0
80	16081.5
81	8800.0
82	8800.0
83	6751.0
84	4702.0
85	4167.0
86	3632.0
87	3184.0
88	2736.0
89	2736.0
90	1957.0
91	1178.0
92	701.5
93	225.0
94	144.5
95	64.0
96	64.0
97	45.0
98	26.0
99	17.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2310121.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.052211318164936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.25732229276312	35.410538225015706
2	7.1107090498947185	5.838206610765315
3	2.1250556729785206	2.6171470364993827
4	0.9711036298169974	1.5946380569233758
5	0.6017615092246735	1.2351820319914577
6	0.4092748958506918	1.0080983707009528
7	0.3070969906853986	0.8824907389752658
8	0.23419336015469533	0.7691324248305328
9	0.18056828755194798	0.6671454748147565
>10	1.3790407743018904	11.443098174673556
>50	0.20075077538395888	5.777160382162833
>100	0.18305721029784644	15.951040815078915
>500	0.028512257261182745	8.028003245635297
>1k	0.011129319748788804	6.982872079135313
>5k	2.1198704283407246E-4	0.6974213054940571
>10k+	2.1198704283407246E-4	1.097825027303161
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14170	0.6133877835836304	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11060	0.47876280073641164	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9989	0.43240159281699964	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	6039	0.2614148782682812	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	4735	0.20496761857928653	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4213	0.18237139959335463	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3175	0.13743868827650155	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2731	0.11821891580570888	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	2560	0.11081670613790359	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2522	0.10917177065616909	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	2510	0.10865231734614768	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2470	0.10692080631274292	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	2334	0.1010336687991668	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	4.3287775835118596E-5	0.0	0.0	0.0	0.0
8	4.3287775835118596E-5	0.0	0.0	0.0	4.3287775835118596E-5
9	4.3287775835118596E-5	4.3287775835118596E-5	0.0	4.3287775835118596E-5	4.3287775835118596E-5
10	4.3287775835118596E-5	4.3287775835118596E-5	0.0	1.7315110334047438E-4	4.3287775835118596E-5
11	4.3287775835118596E-5	8.657555167023719E-5	0.0	3.0301443084583015E-4	4.3287775835118596E-5
12	4.3287775835118596E-5	8.657555167023719E-5	0.0	0.0011254821717130833	4.3287775835118596E-5
13	4.3287775835118596E-5	8.657555167023719E-5	0.0	0.001385208826723795	4.3287775835118596E-5
14	4.3287775835118596E-5	8.657555167023719E-5	0.0	0.0022942521192612854	8.657555167023719E-5
15	4.3287775835118596E-5	8.657555167023719E-5	0.0	0.003289870963469013	8.657555167023719E-5
16	4.3287775835118596E-5	8.657555167023719E-5	0.0	0.005713986410235654	8.657555167023719E-5
17	8.657555167023719E-5	8.657555167023719E-5	0.0	0.009003857373704667	8.657555167023719E-5
18	8.657555167023719E-5	8.657555167023719E-5	0.0	0.010259202872923107	8.657555167023719E-5
19	8.657555167023719E-5	8.657555167023719E-5	0.0	0.011947426130492732	8.657555167023719E-5
20	8.657555167023719E-5	8.657555167023719E-5	0.0	0.013722224939732594	8.657555167023719E-5
21	8.657555167023719E-5	8.657555167023719E-5	0.0	0.01666579369652066	8.657555167023719E-5
22	8.657555167023719E-5	8.657555167023719E-5	0.0	0.022423067882591432	8.657555167023719E-5
23	8.657555167023719E-5	8.657555167023719E-5	0.0	0.02926253646454017	8.657555167023719E-5
24	8.657555167023719E-5	8.657555167023719E-5	0.0	0.041599552577548965	8.657555167023719E-5
25	8.657555167023719E-5	8.657555167023719E-5	0.0	0.04649107124691737	8.657555167023719E-5
26	8.657555167023719E-5	8.657555167023719E-5	0.0	0.05718315187819166	8.657555167023719E-5
27	8.657555167023719E-5	8.657555167023719E-5	0.0	0.09354488357969128	8.657555167023719E-5
28	8.657555167023719E-5	1.298633275053558E-4	0.0	0.23362412618213504	8.657555167023719E-5
29	8.657555167023719E-5	1.7315110334047438E-4	0.0	0.42872213187101454	8.657555167023719E-5
30	8.657555167023719E-5	1.7315110334047438E-4	0.0	0.7094866459375937	8.657555167023719E-5
31	8.657555167023719E-5	1.7315110334047438E-4	0.0	1.3632186365995547	8.657555167023719E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCATCA	1710	0.0	20.447369	14
GACAGGC	1790	0.0	20.256985	7
GCATCAG	1770	0.0	19.963276	15
GCAGTCG	1900	0.0	19.863157	9
GCTGCAT	1785	0.0	19.691877	12
CAGGACA	2030	0.0	19.593596	4
CTGCATC	1810	0.0	19.317678	13
CAGTCGG	1965	0.0	19.300255	10
AGTCGGT	1945	0.0	19.213367	11
CCAGGAC	2015	0.0	19.096775	3
ATCAGAA	1860	0.0	19.096773	17
GGCAGTC	2070	0.0	18.678745	8
ACAGGCT	1940	0.0	18.595362	8
CATCAGA	1945	0.0	18.547558	16
TCCAGGA	2135	0.0	18.370024	2
GGGCAGT	2130	0.0	18.239437	7
TCGGTGA	2130	0.0	17.631454	13
GTGATTC	2095	0.0	17.572792	16
GGTATCA	6665	0.0	17.348087	1
CGGTGAT	2170	0.0	17.221199	14
>>END_MODULE