FastQCFastQC Report
Fri 10 Feb 2017
ERR1631234.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631234.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12269
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA620.5053386584073681No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT590.4808867878392697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT570.4645855407938707No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT460.37492868204417634No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT440.3586274349987774No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC410.3341755644306789No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC380.3097236938625805No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA380.3097236938625805No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA380.3097236938625805No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT380.3097236938625805No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT340.27712119977178257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT310.25266932920368407No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA250.20376558806748718No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT240.1956149645447877TruSeq Adapter, Index 5 (95% over 21bp)
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT220.1793137174993887No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA200.16301247045398973No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT200.16301247045398973No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT200.16301247045398973No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA200.16301247045398973No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC190.15486184693129024No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.15486184693129024No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA190.15486184693129024No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT180.14671122340859075No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT180.14671122340859075No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA180.14671122340859075No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA150.1222593528404923No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG150.1222593528404923No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT140.11410872931779281No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG140.11410872931779281No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA140.11410872931779281No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA130.10595810579509334ABI Solid3 Adapter B (100% over 21bp)
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC130.10595810579509334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGTC250.00528932229.634
CTTATAC358.376536E-426.42857237
CTCTATG1003.9835868E-1022.21
GGTATCA704.4576536E-621.1428591
TCTTATA450.003617116520.55555536
ATGGGAA554.7553715E-420.18181819
TCTATGG1101.3096724E-920.1818182
CAGTCGG656.217926E-519.92307710
GCAGTCG656.217926E-519.9230779
AGTCGGT656.217926E-519.92307711
GGCAGTC656.217926E-519.9230778
TGGGCAG758.102532E-619.7333346
GGGCAGT701.09915076E-418.57
CATGGGA608.5459213E-418.518
ATTCCTC801.4149009E-518.519
GTCGGTG801.4149009E-518.512
ATGGGCA1256.4283086E-917.765
CTATGGG1256.4283086E-917.763
TATGGGC1256.4283086E-917.764
GGTGATT852.3852557E-517.41176415
Produced by FastQC (version 0.11.5)