##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631234.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12269
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65865188686934	34.0	31.0	34.0	31.0	34.0
2	32.82704376884832	34.0	31.0	34.0	31.0	34.0
3	32.839351210367596	34.0	31.0	34.0	31.0	34.0
4	36.28576086070584	37.0	37.0	37.0	35.0	37.0
5	36.25878229684571	37.0	37.0	37.0	35.0	37.0
6	36.32520987855571	37.0	37.0	37.0	35.0	37.0
7	36.34550493112723	37.0	37.0	37.0	35.0	37.0
8	36.31632569891597	37.0	37.0	37.0	35.0	37.0
9	38.13782704376885	39.0	39.0	39.0	37.0	39.0
10	38.10131225038715	39.0	38.0	39.0	37.0	39.0
11	38.16464259515853	39.0	39.0	39.0	37.0	39.0
12	37.9589208574456	39.0	38.0	39.0	35.0	39.0
13	38.0652049881816	39.0	38.0	39.0	37.0	39.0
14	39.50802836416986	41.0	39.0	41.0	37.0	41.0
15	39.479093650664275	41.0	39.0	41.0	37.0	41.0
16	39.397668921672505	40.0	39.0	41.0	37.0	41.0
17	39.31306544950689	40.0	39.0	41.0	36.0	41.0
18	39.37346156981009	41.0	39.0	41.0	37.0	41.0
19	39.40940581954519	41.0	39.0	41.0	37.0	41.0
20	39.439481620343955	41.0	39.0	41.0	37.0	41.0
21	39.43671040834624	41.0	39.0	41.0	37.0	41.0
22	39.385605998858914	41.0	39.0	41.0	36.0	41.0
23	39.32684000326025	41.0	39.0	41.0	36.0	41.0
24	39.27516505012633	41.0	39.0	41.0	36.0	41.0
25	39.2829081424729	41.0	39.0	41.0	36.0	41.0
26	39.151275572581305	41.0	39.0	41.0	36.0	41.0
27	39.09438422039286	41.0	39.0	41.0	35.0	41.0
28	38.99820686282501	40.0	39.0	41.0	35.0	41.0
29	38.88369060233108	40.0	39.0	41.0	35.0	41.0
30	38.75645936914174	40.0	38.0	41.0	35.0	41.0
31	38.65245741299209	40.0	38.0	41.0	35.0	41.0
32	38.50590920205396	40.0	38.0	41.0	35.0	41.0
33	38.359850028527184	40.0	38.0	41.0	34.0	41.0
34	38.32113456679436	40.0	38.0	41.0	34.0	41.0
35	38.22870649604695	40.0	37.0	41.0	34.0	41.0
36	38.11687994131551	40.0	37.0	41.0	34.0	41.0
37	38.0295867633874	40.0	37.0	41.0	33.0	41.0
38	37.87122014834135	40.0	37.0	41.0	33.0	41.0
39	37.683837313554484	40.0	36.0	41.0	33.0	41.0
40	37.620751487488796	40.0	36.0	41.0	33.0	41.0
41	37.43018990952808	40.0	35.0	41.0	33.0	41.0
42	37.26073844649115	39.0	35.0	41.0	33.0	41.0
43	36.32928519031706	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	3.0
22	2.0
23	6.0
24	9.0
25	30.0
26	34.0
27	48.0
28	51.0
29	61.0
30	119.0
31	147.0
32	149.0
33	221.0
34	333.0
35	446.0
36	762.0
37	1494.0
38	2909.0
39	5443.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08166924769745	15.282419105061537	14.467356752791588	28.16855489444943
2	20.604776265384302	18.021028608688564	33.55611704295379	27.818078082973347
3	20.47436628902111	20.31950444208982	28.877659140924283	30.328470127964792
4	14.93194229358546	17.246719374032114	30.255114516260495	37.56622381612193
5	16.80658570380634	35.308501100334176	30.04319830467031	17.841714891189177
6	32.25201727932187	29.969842692966015	18.83609096095851	18.942049066753608
7	32.78995843182003	29.61121525796723	16.912543809601434	20.6862825006113
8	34.615698100904716	27.826228706496046	16.456108892330263	21.10196430026897
9	30.401825739669086	12.943190154046786	15.787757763468905	40.86722634281523
10	21.76216480560763	23.66941070991931	23.34338576901133	31.225038715461732
11	36.57999836987529	23.424892004238323	15.274268481538838	24.720841144347542
12	25.731518461162278	25.747819708207675	23.94653190969109	24.574129920938955
13	36.18061781726302	16.749531339147445	22.422365310946287	24.64748553264325
14	25.3973428967316	19.17026652538919	21.45244111174505	33.97994946613416
15	34.62384872442742	21.615453582199038	17.12446002119162	26.636237672181924
16	28.95101475262858	22.88695085174016	22.756540875376967	25.405493520254304
17	23.799820686282498	26.53843018990953	22.732089004808866	26.929660118999106
18	27.83437933001875	19.83861765425055	22.911402722308257	29.415600293422443
19	26.33466460184204	27.736571847746355	23.17222267503464	22.756540875376967
20	28.942864129105878	20.33580568913522	24.859401744233434	25.86192843752547
21	32.13790855000408	19.015404678457905	22.642432146059175	26.20425462547885
22	31.95044420898199	19.455538348683675	19.422935854592875	29.171081587741458
23	26.962262613089898	21.990382264243213	21.159018664927867	29.88833645773902
24	26.595484554568426	21.501344852881246	23.94653190969109	27.956638682859236
25	26.269459613660445	21.639905452767135	21.949629146629718	30.1410057869427
26	29.798679598989324	20.295052571521722	21.58285108810824	28.323416741380715
27	27.263835683429782	19.643002689705764	21.207922406064064	31.885239220800393
28	26.815551389681307	20.16464259515853	23.865025674464096	29.154780340696064
29	25.72336783763958	20.76778873583829	25.356589779118107	28.152253647404024
30	25.10392044991442	22.585377781400275	26.67699078979542	25.633710978889884
31	26.130899013774556	23.19667454560274	23.34338576901133	27.329040671611377
32	24.64748553264325	22.340859075719294	27.263835683429782	25.747819708207675
33	26.465074578205233	20.80854185345179	26.50582769581873	26.220555872524248
34	27.31273942456598	22.340859075719294	22.658733393104573	27.687668106610158
35	25.69891596707148	23.253728910261636	23.913929415600293	27.13342570706659
36	23.107017686853045	22.104490993561008	26.073844649115657	28.71464667047029
37	23.897628168554892	21.74586355856223	26.08199527263836	28.274513000244518
38	24.525226179802758	18.844241584481214	28.356019235471514	28.274513000244518
39	24.818648626619936	18.159589208574456	29.52155839921754	27.500203765588065
40	23.522699486510717	18.289999184937646	28.470127964789306	29.717173363762328
41	20.286901947999024	20.00163012470454	31.314695574211427	28.39677235308501
42	20.33580568913522	19.18656777243459	30.32031950444209	30.1573070339881
43	18.371505420164645	21.199771782541365	33.27084521965931	27.157877577634686
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	3.0
4	5.0
5	5.0
6	9.0
7	13.0
8	13.0
9	13.0
10	13.0
11	13.0
12	13.0
13	36.5
14	60.0
15	83.0
16	106.0
17	90.0
18	74.0
19	74.0
20	93.5
21	113.0
22	80.5
23	48.0
24	47.5
25	47.0
26	47.0
27	41.0
28	35.0
29	50.5
30	66.0
31	72.0
32	78.0
33	78.0
34	86.0
35	94.0
36	116.5
37	139.0
38	169.0
39	199.0
40	199.0
41	240.5
42	282.0
43	363.0
44	444.0
45	625.5
46	807.0
47	807.0
48	1106.5
49	1406.0
50	1386.0
51	1366.0
52	1191.0
53	1016.0
54	1016.0
55	902.5
56	789.0
57	811.0
58	833.0
59	796.5
60	760.0
61	760.0
62	760.5
63	761.0
64	741.0
65	721.0
66	670.0
67	619.0
68	619.0
69	553.0
70	487.0
71	436.0
72	385.0
73	304.5
74	224.0
75	224.0
76	175.0
77	126.0
78	99.5
79	73.0
80	56.0
81	39.0
82	39.0
83	27.5
84	16.0
85	10.0
86	4.0
87	3.0
88	2.0
89	2.0
90	2.5
91	3.0
92	1.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	12269.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.25861928437526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.00881057268722	78.27043768848317
2	3.445912873225648	5.738038959980439
3	1.0181106216348508	2.54299453908224
4	0.3622124326970142	1.206292281359524
5	0.27410670582476754	1.1410872931779281
6	0.14684287812041116	0.7335561170429538
7	0.14684287812041116	0.8558154698834461
8	0.1076847772883015	0.7172548699975548
9	0.02936857562408223	0.22006683511288616
>10	0.430739109153206	7.123644958839351
>50	0.02936857562408223	1.4508109870405086
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	62	0.5053386584073681	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	59	0.4808867878392697	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	57	0.4645855407938707	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	46	0.37492868204417634	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	44	0.3586274349987774	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	41	0.3341755644306789	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	38	0.3097236938625805	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	38	0.3097236938625805	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	38	0.3097236938625805	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	38	0.3097236938625805	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	34	0.27712119977178257	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31	0.25266932920368407	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	25	0.20376558806748718	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	24	0.1956149645447877	TruSeq Adapter, Index 5 (95% over 21bp)
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	22	0.1793137174993887	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	20	0.16301247045398973	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTAT	20	0.16301247045398973	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	20	0.16301247045398973	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	20	0.16301247045398973	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	19	0.15486184693129024	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.15486184693129024	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	19	0.15486184693129024	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	18	0.14671122340859075	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	18	0.14671122340859075	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	18	0.14671122340859075	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	15	0.1222593528404923	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	15	0.1222593528404923	No Hit
TCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCAT	14	0.11410872931779281	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	14	0.11410872931779281	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	14	0.11410872931779281	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	13	0.10595810579509334	ABI Solid3 Adapter B (100% over 21bp)
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	13	0.10595810579509334	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.008150623522699487	0.0
18	0.0	0.0	0.0	0.008150623522699487	0.0
19	0.0	0.0	0.0	0.008150623522699487	0.0
20	0.0	0.0	0.0	0.008150623522699487	0.0
21	0.0	0.0	0.0	0.008150623522699487	0.0
22	0.0	0.0	0.0	0.008150623522699487	0.0
23	0.0	0.0	0.0	0.03260249409079795	0.0
24	0.0	0.0	0.0	0.04075311761349743	0.0
25	0.0	0.0	0.0	0.04890374113619692	0.0
26	0.0	0.0	0.0	0.07335561170429537	0.0
27	0.0	0.0	0.0	0.11410872931779281	0.0
28	0.0	0.0	0.0	0.2608199527263836	0.0
29	0.0	0.0	0.0	0.5949955171570626	0.0
30	0.0	0.0	0.0	0.9862254462466379	0.0
31	0.0	0.0	0.0	1.9235471513570788	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACTGTC	25	0.005289322	29.6	34
CTTATAC	35	8.376536E-4	26.428572	37
CTCTATG	100	3.9835868E-10	22.2	1
GGTATCA	70	4.4576536E-6	21.142859	1
TCTTATA	45	0.0036171165	20.555555	36
ATGGGAA	55	4.7553715E-4	20.181818	19
TCTATGG	110	1.3096724E-9	20.181818	2
CAGTCGG	65	6.217926E-5	19.923077	10
GCAGTCG	65	6.217926E-5	19.923077	9
AGTCGGT	65	6.217926E-5	19.923077	11
GGCAGTC	65	6.217926E-5	19.923077	8
TGGGCAG	75	8.102532E-6	19.733334	6
GGGCAGT	70	1.09915076E-4	18.5	7
CATGGGA	60	8.5459213E-4	18.5	18
ATTCCTC	80	1.4149009E-5	18.5	19
GTCGGTG	80	1.4149009E-5	18.5	12
ATGGGCA	125	6.4283086E-9	17.76	5
CTATGGG	125	6.4283086E-9	17.76	3
TATGGGC	125	6.4283086E-9	17.76	4
GGTGATT	85	2.3852557E-5	17.411764	15
>>END_MODULE