FastQCFastQC Report
Fri 10 Feb 2017
ERR1631233.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631233.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136473
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2570.18831563752537134No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC2380.17439346977057732No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC2070.151678353960124No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT1860.1362906948627201No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1650.12090303576531622No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1480.10844635935313213No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGATT200.001836257937.04
AGGGACG200.001836257937.08
TAAGAGG250.005479593829.64
GTAGTGA250.005479593829.617
CTGCATC851.8189894E-1228.29411913
TGACCCG358.8292296E-426.42857213
ATCAGAA856.730261E-1126.11764717
GCTGCAT856.730261E-1126.11764712
TGCATCA856.730261E-1126.11764714
TCCAGGA1000.025.9000022
GACAGGC809.604264E-1025.43757
TCAGAAG901.4006218E-1024.66666818
TATACAC901.4006218E-1024.66666837
GCATCAG901.4006218E-1024.66666815
CATCAGA952.746674E-1023.36842216
ATGCTCT400.001922525223.12532
GGCAGTC903.754394E-922.6111138
CAGAAGA1005.2204996E-1022.219
AGAAGAG1005.2204996E-1022.220
GTTCAAA502.685229E-422.21
Produced by FastQC (version 0.11.5)