FastQCFastQC Report
Fri 10 Feb 2017
ERR1631232.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631232.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379033
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC58360.4231950939535167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51630.3743927810284453No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT49660.3601074085971837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41360.29992030647562457No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34870.25285834349141756No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG26110.18933557064986842No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG25750.1867250457385719No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT25620.18578235618727035No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT24020.17418002324817464No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT23200.16823382761688807No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG22420.1625776903090789No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA21840.1583718446186567No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG20950.1519180469212847No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG19890.1442315013491338No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT19730.1430712680552242No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC18920.137197587004807No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG18060.13096133305004304No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG17760.1287858956239626No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT17710.12842332271961585No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA16700.12109935005181166No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA15810.11464555235443967No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC14660.10630637555446461No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT14610.10594380265011787No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC14510.1052186568414244No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG14380.10427596729012285No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT14340.10398590896664547No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14200.10297070483447458No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC14110.10231807360665046No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT14080.10210052986404242No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG14050.10188298612143437No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC13810.10014263618057001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAT358.8702975E-426.4285721
GTACTAG358.8702975E-426.4285721
GAACCGT358.8702975E-426.4285726
ATACACA4600.023.72825837
CGCACTA400.001931381423.12500212
TGCATCA14550.023.01374614
TATTGAC2750.022.87272630
GCATCAG14800.022.62515
TATCGTT753.7418977E-722.213
GTGTTAT502.7024825E-422.1999991
CTGCATC15200.022.02960613
ACCGTTA1107.4578566E-1121.8636368
GCTGCAT15500.021.72258212
CAGGACA17050.021.5923754
ACTGTGT603.726982E-521.5833328
GATACAC1059.840733E-1021.1428573
GACAGGC16550.020.4561947
CATCAGA16550.020.34441216
ATCAGAA16600.020.28313317
TAATCGG555.143809E-420.18181816
Produced by FastQC (version 0.11.5)