##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631232.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1379033
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80046235296762	34.0	31.0	34.0	31.0	34.0
2	32.97156775798694	34.0	33.0	34.0	31.0	34.0
3	33.04972107266469	34.0	33.0	34.0	31.0	34.0
4	36.40219632162537	37.0	37.0	37.0	35.0	37.0
5	36.39991283747379	37.0	37.0	37.0	35.0	37.0
6	36.44968031947024	37.0	37.0	37.0	35.0	37.0
7	36.43827740162853	37.0	37.0	37.0	35.0	37.0
8	36.42100080273641	37.0	37.0	37.0	35.0	37.0
9	38.27285206372871	39.0	39.0	39.0	37.0	39.0
10	38.23453390890573	39.0	39.0	39.0	37.0	39.0
11	38.29158112967565	39.0	39.0	39.0	37.0	39.0
12	38.07572987738509	39.0	39.0	39.0	37.0	39.0
13	38.180647598715915	39.0	39.0	39.0	37.0	39.0
14	39.67605996375721	41.0	40.0	41.0	37.0	41.0
15	39.684148965253186	41.0	40.0	41.0	37.0	41.0
16	39.65707347104819	41.0	40.0	41.0	37.0	41.0
17	39.62996462013599	41.0	39.0	41.0	37.0	41.0
18	39.56796247805527	41.0	40.0	41.0	37.0	41.0
19	39.599688332331425	41.0	40.0	41.0	37.0	41.0
20	39.59755857909129	41.0	40.0	41.0	37.0	41.0
21	39.579117396030405	41.0	40.0	41.0	37.0	41.0
22	39.516340798226004	41.0	39.0	41.0	37.0	41.0
23	39.44648170130809	41.0	39.0	41.0	36.0	41.0
24	39.40303749076345	41.0	39.0	41.0	36.0	41.0
25	39.36934359076251	41.0	39.0	41.0	36.0	41.0
26	39.26561655884957	41.0	39.0	41.0	36.0	41.0
27	39.19624330962348	41.0	39.0	41.0	35.0	41.0
28	39.07981824945451	41.0	39.0	41.0	35.0	41.0
29	38.970235665136364	40.0	39.0	41.0	35.0	41.0
30	38.890598702134035	40.0	38.0	41.0	35.0	41.0
31	38.839412834935786	40.0	38.0	41.0	35.0	41.0
32	38.741071460944006	40.0	38.0	41.0	35.0	41.0
33	38.623961863131626	40.0	38.0	41.0	35.0	41.0
34	38.54928562260657	40.0	38.0	41.0	35.0	41.0
35	38.40122172565849	40.0	38.0	41.0	34.0	41.0
36	38.351692091487294	40.0	38.0	41.0	34.0	41.0
37	38.25866966200229	40.0	38.0	41.0	34.0	41.0
38	38.16058063875194	40.0	37.0	41.0	34.0	41.0
39	38.044398502428876	40.0	37.0	41.0	34.0	41.0
40	37.89706410216434	40.0	37.0	41.0	33.0	41.0
41	37.76382001010853	40.0	37.0	41.0	33.0	41.0
42	37.63447647735769	40.0	36.0	41.0	33.0	41.0
43	36.61627459241367	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	2.0
13	5.0
14	1.0
15	4.0
16	4.0
17	9.0
18	20.0
19	64.0
20	107.0
21	242.0
22	434.0
23	702.0
24	1119.0
25	1820.0
26	2583.0
27	3811.0
28	5286.0
29	7294.0
30	9677.0
31	12635.0
32	16029.0
33	22232.0
34	34694.0
35	53718.0
36	77174.0
37	141100.0
38	300915.0
39	687345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.594364311804	14.85794756180599	12.356992182203037	28.190695944186977
2	21.675840969722987	17.213438692185033	32.452450376459446	28.658269961632538
3	21.68903862344121	18.95030793316766	27.477732585079544	31.882920858311586
4	16.18025094395856	14.708495010634262	29.728440146102376	39.3828138993048
5	17.686306274034052	33.73073740802432	30.047286758184903	18.53566955975673
6	37.25066767800335	29.612199272968816	15.59455067427683	17.542582374751003
7	33.29347448538215	27.450032015187453	17.36702457446631	21.88946892496409
8	31.55174676748127	29.53910457545251	17.03454522118035	21.87460343588587
9	29.56695017450634	13.200699330617905	16.201570230734145	41.03078026414161
10	21.722757903545457	23.2391103041044	26.14999060936178	28.88814118298837
11	38.616334779515796	20.713499967005866	17.103941675072313	23.566223578406028
12	26.838008952650156	23.285157062956436	23.466153456806328	26.410680527587083
13	33.52610126081102	16.28786258196867	22.423103725581623	27.762932431638692
14	25.31295480238689	18.40847898491189	21.41377327446116	34.86479293824006
15	30.586432666948504	22.686259139556487	18.760609789613447	27.966698403881562
16	28.20708423946345	22.59119252403677	19.874723810090114	29.326999426409667
17	26.41321853791751	24.306742478243812	21.77823155791051	27.50180742592817
18	26.82959726126931	20.20335989059	23.488487947714088	29.4785549004266
19	27.692883346518904	22.96043676982349	22.22745938639612	27.119220497261487
20	28.1173111883472	21.024877577258845	23.016925628320713	27.84088560607324
21	32.22881540905838	20.07421142206169	21.338503139518778	26.358470029361154
22	31.116731796846054	20.949897500639942	20.181678030910067	27.751692671603944
23	28.782777496985208	21.707602356143763	20.51002405308647	28.999596093784557
24	28.265458477063277	21.59440709540671	22.383655793588698	27.756478633941317
25	27.565475227931458	20.851277670657627	22.02130043298456	29.56194666842635
26	29.527284698770806	21.223059926774777	22.305557589992407	26.944097784462013
27	29.805668174728233	20.33453876738265	20.9533056859408	28.906487371948312
28	27.25039937405414	20.185376274534402	23.53787037728611	29.026353974125346
29	27.072666136343365	20.645843863054765	24.37171554270275	27.909774457899122
30	26.06377077270812	21.43516507581762	24.776927020600667	27.72413713087359
31	26.901459210910833	22.025723822417593	22.49424052941445	28.57857643725712
32	25.9206995046529	21.3192867755884	24.577656952371697	28.182356767387002
33	27.636031915117332	21.032709151992737	23.838950917055644	27.492308015834283
34	27.999329965272768	21.533712391219066	22.94636894113484	27.520588702373328
35	27.372296384495513	21.139668158775027	24.229006847551872	27.259028609177587
36	25.83810539704271	21.346842316318753	23.494289114183633	29.3207631724549
37	25.0555280403007	20.549689528822007	25.214625030728055	29.180157400149238
38	25.827155695331438	18.845379334649714	26.671370445812393	28.656094524206456
39	24.707095479223483	19.15088326385228	27.62718513625127	28.514836120672964
40	24.539079195349206	18.348001824466856	28.30287600079186	28.81004297939208
41	22.107737813380826	19.249285550091987	30.078177969635245	28.564798666891946
42	22.116512077666016	19.71163851771495	29.074793714146075	29.097055690472963
43	20.863822693148023	18.791573515644657	30.653218595929175	29.69138519527814
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	55.0
2	85.0
3	252.0
4	419.0
5	419.0
6	614.0
7	809.0
8	872.5
9	936.0
10	1430.0
11	1924.0
12	1924.0
13	3315.5
14	4707.0
15	7417.5
16	10128.0
17	8877.5
18	7627.0
19	7627.0
20	8373.5
21	9120.0
22	6564.5
23	4009.0
24	3712.0
25	3415.0
26	3415.0
27	3734.0
28	4053.0
29	5190.5
30	6328.0
31	7875.0
32	9422.0
33	9422.0
34	12532.0
35	15642.0
36	19472.5
37	23303.0
38	27778.5
39	32254.0
40	32254.0
41	37322.5
42	42391.0
43	49704.5
44	57018.0
45	64743.5
46	72469.0
47	72469.0
48	85235.5
49	98002.0
50	103084.5
51	108167.0
52	112308.0
53	116449.0
54	116449.0
55	114254.0
56	112059.0
57	107859.5
58	103660.0
59	96941.5
60	90223.0
61	90223.0
62	92046.5
63	93870.0
64	88018.0
65	82166.0
66	73561.5
67	64957.0
68	64957.0
69	56732.0
70	48507.0
71	44806.5
72	41106.0
73	42003.0
74	42900.0
75	42900.0
76	40700.5
77	38501.0
78	30242.0
79	21983.0
80	13764.0
81	5545.0
82	5545.0
83	3919.5
84	2294.0
85	1794.5
86	1295.0
87	1006.0
88	717.0
89	717.0
90	552.0
91	387.0
92	250.0
93	113.0
94	72.0
95	31.0
96	31.0
97	23.0
98	15.0
99	8.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1379033.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.43616910410875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.81306129500976	33.890791196580466
2	8.061188872748804	6.519271928772609
3	2.4998839846901344	3.0325719463675043
4	1.3562992340935027	2.1937418074231223
5	0.7679904167154827	1.5527295180321101
6	0.5459736430924859	1.3246249535084444
7	0.3753700672705776	1.0624969261741626
8	0.2774381094838712	0.8974827448811242
9	0.23015731135820594	0.8376011966364684
>10	1.6462270298961221	13.174317548494518
>50	0.2154754554418513	6.180213289738907
>100	0.17340302217222642	14.447131353635076
>500	0.027246467606086216	7.490026769506961
>1k	0.009924210055196968	6.594477711312733
>5k	3.608803656435261E-4	0.8025211089358244
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	5836	0.4231950939535167	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5163	0.3743927810284453	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	4966	0.3601074085971837	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4136	0.29992030647562457	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3487	0.25285834349141756	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	2611	0.18933557064986842	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	2575	0.1867250457385719	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	2562	0.18578235618727035	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	2402	0.17418002324817464	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	2320	0.16823382761688807	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	2242	0.1625776903090789	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	2184	0.1583718446186567	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	2095	0.1519180469212847	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	1989	0.1442315013491338	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	1973	0.1430712680552242	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	1892	0.137197587004807	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	1806	0.13096133305004304	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	1776	0.1287858956239626	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	1771	0.12842332271961585	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1670	0.12109935005181166	No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA	1581	0.11464555235443967	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1466	0.10630637555446461	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	1461	0.10594380265011787	No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC	1451	0.1052186568414244	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	1438	0.10427596729012285	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	1434	0.10398590896664547	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1420	0.10297070483447458	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1411	0.10231807360665046	No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT	1408	0.10210052986404242	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	1405	0.10188298612143437	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	1381	0.10014263618057001	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	7.25145808693483E-5	0.0
11	0.0	0.0	0.0	2.175437426080449E-4	0.0
12	0.0	0.0	0.0	2.175437426080449E-4	0.0
13	0.0	0.0	0.0	2.175437426080449E-4	0.0
14	0.0	0.0	0.0	2.175437426080449E-4	0.0
15	0.0	0.0	0.0	3.625729043467415E-4	0.0
16	0.0	0.0	0.0	0.0011602332939095728	0.0
17	0.0	0.0	0.0	0.001667835359995011	0.0
18	0.0	0.0	0.0	0.0018128645217337076	0.0
19	0.0	0.0	0.0	0.0025380103304271906	0.0
20	0.0	0.0	0.0	0.0030456123965126288	0.0
21	0.0	0.0	0.0	0.004205845690422202	0.0
22	0.0	0.0	0.0	0.007178943506065482	0.0
23	7.25145808693483E-5	0.0	0.0	0.011674847519965077	0.0
24	7.25145808693483E-5	0.0	0.0	0.020304082643417525	0.0
25	7.25145808693483E-5	0.0	0.0	0.02530758872340256	0.0
26	7.25145808693483E-5	0.0	0.0	0.037272494566845026	0.0
27	7.25145808693483E-5	0.0	0.0	0.09216603228494169	7.25145808693483E-5
28	1.450291617386966E-4	0.0	0.0	0.3314641491537911	1.450291617386966E-4
29	1.450291617386966E-4	0.0	0.0	0.6710499313649492	1.450291617386966E-4
30	1.450291617386966E-4	0.0	0.0	1.1304298011722707	1.450291617386966E-4
31	1.450291617386966E-4	0.0	0.0	2.1450538166961923	1.450291617386966E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATAT	35	8.8702975E-4	26.428572	1
GTACTAG	35	8.8702975E-4	26.428572	1
GAACCGT	35	8.8702975E-4	26.428572	6
ATACACA	460	0.0	23.728258	37
CGCACTA	40	0.0019313814	23.125002	12
TGCATCA	1455	0.0	23.013746	14
TATTGAC	275	0.0	22.872726	30
GCATCAG	1480	0.0	22.625	15
TATCGTT	75	3.7418977E-7	22.2	13
GTGTTAT	50	2.7024825E-4	22.199999	1
CTGCATC	1520	0.0	22.029606	13
ACCGTTA	110	7.4578566E-11	21.863636	8
GCTGCAT	1550	0.0	21.722582	12
CAGGACA	1705	0.0	21.592375	4
ACTGTGT	60	3.726982E-5	21.583332	8
GATACAC	105	9.840733E-10	21.142857	3
GACAGGC	1655	0.0	20.456194	7
CATCAGA	1655	0.0	20.344412	16
ATCAGAA	1660	0.0	20.283133	17
TAATCGG	55	5.143809E-4	20.181818	16
>>END_MODULE