FastQCFastQC Report
Fri 10 Feb 2017
ERR1631231.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631231.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43664
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA650.14886405276658118No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC520.11909124221326493No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC500.11451080982044705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT480.10993037742762918No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC460.10534994503481128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCT250.00543932629.5999984
ACGCTGA250.00543932629.5999986
GCACACG250.00543932629.5999982
GGTATCA809.1495167E-1025.4375021
CTTATAC1251.6370905E-1120.7237
GTATCAA1002.7210444E-718.4999982
CAACGCA1058.9385085E-615.8571426
TCAACGC1101.3892864E-515.1363645
ATCAACG1101.3892864E-515.1363644
TCTTATA1753.265268E-813.74285836
AACGCAG1254.6393343E-513.327
TATCAAC1409.066864E-613.2142853
CAGAGTA1306.703321E-512.80769311
GCAGAGT1306.703321E-512.80769310
ACGCAGA1359.543268E-512.3333348
AGAGTAC1401.3401234E-411.89285712
CTCTTAT2653.6743586E-1011.86792537
TCTCTTA3750.011.83999937
CGCAGAG1451.858025E-411.48275859
GAGTACA1150.00508311811.26086913
Produced by FastQC (version 0.11.5)