##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631231.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43664
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.808721143275925	34.0	31.0	34.0	31.0	34.0
2	32.99294613411506	34.0	33.0	34.0	31.0	34.0
3	33.10218944668377	34.0	33.0	34.0	31.0	34.0
4	36.413269512641996	37.0	37.0	37.0	35.0	37.0
5	36.40630725540491	37.0	37.0	37.0	35.0	37.0
6	36.460928911689265	37.0	37.0	37.0	35.0	37.0
7	36.44448515939905	37.0	37.0	37.0	35.0	37.0
8	36.43298827409308	37.0	37.0	37.0	35.0	37.0
9	38.27271894466838	39.0	39.0	39.0	37.0	39.0
10	38.234678453646026	39.0	39.0	39.0	37.0	39.0
11	38.2973845731037	39.0	39.0	39.0	37.0	39.0
12	38.065225357273725	39.0	39.0	39.0	37.0	39.0
13	38.20536368633199	39.0	39.0	39.0	37.0	39.0
14	39.72929644558446	41.0	40.0	41.0	38.0	41.0
15	39.73128893367534	41.0	40.0	41.0	38.0	41.0
16	39.702821546353974	41.0	40.0	41.0	37.0	41.0
17	39.69448515939905	41.0	40.0	41.0	38.0	41.0
18	39.663567240747525	41.0	40.0	41.0	37.0	41.0
19	39.69975265665079	41.0	40.0	41.0	37.0	41.0
20	39.66649871747893	41.0	40.0	41.0	37.0	41.0
21	39.63631366801026	41.0	40.0	41.0	37.0	41.0
22	39.59007420300476	41.0	40.0	41.0	37.0	41.0
23	39.530436973250275	41.0	39.0	41.0	37.0	41.0
24	39.47347929644558	41.0	39.0	41.0	37.0	41.0
25	39.42744595089776	41.0	39.0	41.0	37.0	41.0
26	39.36737358006596	41.0	39.0	41.0	36.0	41.0
27	39.316484976181755	41.0	39.0	41.0	36.0	41.0
28	39.23000641260535	41.0	39.0	41.0	36.0	41.0
29	39.160864785635766	41.0	39.0	41.0	36.0	41.0
30	39.08130267497252	41.0	39.0	41.0	35.0	41.0
31	39.03323103700989	40.0	39.0	41.0	35.0	41.0
32	38.956921033345544	40.0	39.0	41.0	35.0	41.0
33	38.87035086112129	40.0	38.0	41.0	35.0	41.0
34	38.819805789666546	40.0	38.0	41.0	35.0	41.0
35	38.72485342616343	40.0	38.0	41.0	35.0	41.0
36	38.68214089410041	40.0	38.0	41.0	35.0	41.0
37	38.59983052400147	40.0	38.0	41.0	35.0	41.0
38	38.5186881641627	40.0	38.0	41.0	35.0	41.0
39	38.423827409307435	40.0	38.0	41.0	35.0	41.0
40	38.357434041773544	40.0	38.0	41.0	34.0	41.0
41	38.312820630267495	40.0	37.0	41.0	34.0	41.0
42	38.19345456211067	40.0	37.0	41.0	34.0	41.0
43	37.28458226456578	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	10.0
22	9.0
23	22.0
24	34.0
25	36.0
26	75.0
27	108.0
28	130.0
29	204.0
30	262.0
31	352.0
32	427.0
33	623.0
34	939.0
35	1360.0
36	2249.0
37	4187.0
38	9306.0
39	23323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.82246244045438	17.316324661048004	12.43129351410773	27.429919384389883
2	21.828050567973616	18.667552216929277	30.592707951630633	28.91168926346647
3	21.271528032246245	19.88136680102602	27.597105166727737	31.25
4	16.198699157200437	15.390252839868085	30.956852326859657	37.45419567607182
5	18.29195676071821	32.59435690729205	31.172132649322098	17.941553682667642
6	37.252656650787834	31.604983510443386	14.474166361304508	16.66819347746427
7	31.91416269695859	27.253572737266396	19.345456211066324	21.486808354708682
8	29.47508244778307	29.69494320263833	18.330890436057164	22.499083913521435
9	27.434499816782704	13.457310370098938	18.111029681201906	40.99716013191645
10	19.89739831440088	23.90069622572371	29.204836936606814	26.997068523268595
11	39.391718578233785	19.883657017222426	18.452271894466836	22.27235251007695
12	24.640436057163797	23.477006229388053	24.947325027482595	26.935232685965556
13	32.530230853792595	17.14684866251374	22.366251374129718	27.956669109563943
14	25.009160864785635	19.0568889703188	21.09976181751557	34.83418834737999
15	28.82466104800293	24.738915353609382	18.894283620373763	27.542139978013925
16	27.965829974349575	23.115152070355443	21.587577867350678	27.3314400879443
17	26.910040307805055	23.815958226456576	21.896757053865883	27.377244411872482
18	27.471143275925247	21.484518138512275	23.275467204104068	27.76887138145841
19	27.55588127519238	23.037284719677537	21.88301575668743	27.523818248442655
20	28.103242946134117	21.736441920117258	22.288384023451812	27.87193111029681
21	28.95520337119824	22.070813484792964	22.400604617075853	26.573378526932945
22	29.262092341517036	21.855533162330524	21.507420300476365	27.37495419567607
23	27.498625870282154	21.924239648222795	21.899047270062294	28.678087211432757
24	27.15967387321363	22.75558812751924	21.9517222425797	28.133015756687428
25	27.063484792964452	22.07539391718578	22.219677537559544	28.64144375229022
26	27.816965921582998	22.487632832539393	22.29525467204104	27.40014657383657
27	27.915445218028584	21.599028948332723	22.308995969219495	28.1765298644192
28	26.65811652620007	21.956302674972516	23.35333455478197	28.03224624404544
29	26.00311469402712	21.958592891168927	23.758702821546354	28.279589593257604
30	25.600036643459145	22.4899230487358	24.237358006595823	27.672682301209235
31	27.487174789300113	22.499083913521435	22.23112861854159	27.782612678636866
32	25.996244045437887	22.194485159399047	23.465555148406008	28.343715646757055
33	25.375595456211066	21.4135214364236	24.251099303774275	28.959783803591062
34	26.552766581165262	21.138695492854527	24.46408941004031	27.844448515939906
35	26.564217662147303	20.815775009160863	24.903810919750825	27.716196408941002
36	24.983968486625137	21.008153169659217	25.567973616709416	28.439904727006226
37	25.677903994137047	19.482869182850862	26.68330890436057	28.155917918651518
38	24.427445950897763	18.33318065225357	28.659765481861488	28.579607914987175
39	23.973983144008795	18.520978380359107	29.491113961157932	28.013924514474166
40	22.982319530963725	18.802674972517405	30.11634298277757	28.098662513741296
41	21.853242946134117	19.643184316599488	30.851502381824847	27.652070355441555
42	21.296720410406742	20.112678636863322	30.76905459875412	27.82154635397582
43	19.819530963722976	19.96152436790033	31.208776108464637	29.01016855991206
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	2.5
4	5.0
5	5.0
6	8.5
7	12.0
8	9.5
9	7.0
10	12.5
11	18.0
12	18.0
13	34.5
14	51.0
15	74.5
16	98.0
17	96.5
18	95.0
19	95.0
20	108.0
21	121.0
22	104.0
23	87.0
24	98.5
25	110.0
26	110.0
27	144.0
28	178.0
29	233.5
30	289.0
31	377.5
32	466.0
33	466.0
34	584.0
35	702.0
36	888.5
37	1075.0
38	1262.0
39	1449.0
40	1449.0
41	1586.5
42	1724.0
43	1982.0
44	2240.0
45	2461.5
46	2683.0
47	2683.0
48	2944.5
49	3206.0
50	3353.5
51	3501.0
52	3575.0
53	3649.0
54	3649.0
55	3606.0
56	3563.0
57	3517.0
58	3471.0
59	3382.5
60	3294.0
61	3294.0
62	3080.0
63	2866.0
64	2709.0
65	2552.0
66	2274.0
67	1996.0
68	1996.0
69	1744.0
70	1492.0
71	1264.0
72	1036.0
73	790.0
74	544.0
75	544.0
76	460.5
77	377.0
78	315.5
79	254.0
80	223.5
81	193.0
82	193.0
83	145.5
84	98.0
85	85.0
86	72.0
87	59.5
88	47.0
89	47.0
90	37.5
91	28.0
92	17.5
93	7.0
94	6.5
95	6.0
96	6.0
97	3.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	43664.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.10113594723342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.76798825256975	75.23589226823012
2	3.7473172935728	6.07823378526933
3	1.0787303738845588	2.6245877610846464
4	0.6353778380210099	2.0611945767680466
5	0.4179374223427087	1.6947599853426163
6	0.2315599231898791	1.126786368633199
7	0.2739184457246131	1.555056797361671
8	0.17790579464588274	1.1542689629901062
9	0.11860386309725517	0.8657017222425798
>10	0.5450129899469106	7.335562477097839
>50	0.005647803004631199	0.2679552949798461
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	65	0.14886405276658118	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	52	0.11909124221326493	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	50	0.11451080982044705	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	48	0.10993037742762918	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	46	0.10534994503481128	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.002290216196408941	0.0
17	0.0	0.0	0.0	0.002290216196408941	0.0
18	0.0	0.0	0.0	0.002290216196408941	0.0
19	0.0	0.0	0.0	0.002290216196408941	0.0
20	0.0	0.0	0.0	0.002290216196408941	0.0
21	0.0	0.0	0.0	0.009160864785635764	0.0
22	0.0	0.0	0.0	0.009160864785635764	0.0
23	0.0	0.0	0.0	0.011451080982044705	0.0
24	0.0	0.0	0.0	0.029772810553316233	0.0
25	0.0	0.0	0.0	0.04580432392817882	0.0
26	0.0	0.0	0.0	0.08015756687431294	0.0
27	0.0	0.0	0.0	0.1900879443019421	0.0
28	0.0	0.0	0.0	0.4969769146207402	0.0
29	0.0	0.0	0.0	0.895474532795896	0.0
30	0.0	0.0	0.0	1.4268046903627702	0.0
31	0.0	0.0	0.0	2.5352693294246977	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGCT	25	0.005439326	29.599998	4
ACGCTGA	25	0.005439326	29.599998	6
GCACACG	25	0.005439326	29.599998	2
GGTATCA	80	9.1495167E-10	25.437502	1
CTTATAC	125	1.6370905E-11	20.72	37
GTATCAA	100	2.7210444E-7	18.499998	2
CAACGCA	105	8.9385085E-6	15.857142	6
TCAACGC	110	1.3892864E-5	15.136364	5
ATCAACG	110	1.3892864E-5	15.136364	4
TCTTATA	175	3.265268E-8	13.742858	36
AACGCAG	125	4.6393343E-5	13.32	7
TATCAAC	140	9.066864E-6	13.214285	3
CAGAGTA	130	6.703321E-5	12.807693	11
GCAGAGT	130	6.703321E-5	12.807693	10
ACGCAGA	135	9.543268E-5	12.333334	8
AGAGTAC	140	1.3401234E-4	11.892857	12
CTCTTAT	265	3.6743586E-10	11.867925	37
TCTCTTA	375	0.0	11.839999	37
CGCAGAG	145	1.858025E-4	11.4827585	9
GAGTACA	115	0.005083118	11.260869	13
>>END_MODULE