FastQCFastQC Report
Fri 10 Feb 2017
ERR1631230.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631230.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121113
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC2200.18164854309611686No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA2070.17091476554952814No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1710.14119045849743628No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1670.13788775771387052No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC1540.1271539801672818No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1360.11229182664123587No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1310.10816345066177867No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT1290.10651210026999579No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC1230.10155804909464715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA901.382432E-1024.6666681
TGAACAG551.8845014E-523.5454545
CTTATAC1700.021.76470637
CGGCGTC701.20780795E-418.514
ATTAACC609.1719354E-418.534
GTCGGCG609.1719354E-418.512
GGCGTCC752.0480914E-417.26666615
CGGAATT650.001568926617.07692330
TCGGCGT650.001568926617.07692313
TCGGAAT650.001568926617.07692329
GTATCAA1301.3575118E-817.0769232
ATCGGAA650.001568926617.07692328
TATCGGA650.001568926617.07692327
TCGTTAT700.00257458115.85714223
TTCGTTA700.00257458115.85714222
CGTTCGT700.00257458115.85714220
AGCGGTC700.00257458115.8571428
TTAACCA700.00257458115.85714235
GAATTAA700.00257458115.85714232
TCTTATA2800.015.19642837