FastQCFastQC Report
Fri 10 Feb 2017
ERR1631227.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631227.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1238
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40.32310177705977383No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT30.24232633279483037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30.24232633279483037No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA30.24232633279483037No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA30.24232633279483037No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC30.24232633279483037RNA PCR Primer, Index 46 (95% over 22bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT30.24232633279483037RNA PCR Primer, Index 46 (95% over 21bp)
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT20.16155088852988692No Hit
ATTAAAAACACAGCACTCTGCAAACACGAAAGTGGACGTATAG20.16155088852988692No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.16155088852988692No Hit
GTGGTCCGGCATCCGCCGCGACTACACGGCCGAGGATGTCGTG20.16155088852988692No Hit
CCGGAAGTGGCCGCAAAGCTGGCCGATCGCTTCATGCAGGTGC20.16155088852988692No Hit
TACCAAATGTACCCATTTGTCCACCTTGACCCCTGTCTCTTAT20.16155088852988692No Hit
CCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCTGTCTC20.16155088852988692No Hit
TCGTCGACGTCGACCATGAATGGCCGTGGACCTGTCTCTTATA20.16155088852988692No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG20.16155088852988692No Hit
GGTATCAACGCAGAGTACATGGGAAGAGTGGTATCAACGCCTG20.16155088852988692No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.16155088852988692No Hit
GGCCAGCCCACTTCGCCGCCGCTGAAGGCCGAGCACTTCGTCA20.16155088852988692No Hit
CGCTTCTTCGAGGGCCGCAACAGCATCGACTTCGAGACTGTCT20.16155088852988692No Hit
GGCCTGGCCCGCTGCACATCCATGGCCAGCGAGAACAGCGAGT20.16155088852988692No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT20.16155088852988692No Hit
GCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGCTG20.16155088852988692No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA20.16155088852988692No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA20.16155088852988692ABI Solid3 Adapter B (100% over 21bp)
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC20.16155088852988692No Hit
GTCGGTGATTCCTCGCCTTATAGATATGCCCATAGAGCTGTCT20.16155088852988692No Hit
AACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGC20.16155088852988692No Hit
TCAATAAGCGGAGGAATCACCGACTGCCCATAGAGAGGGGAAA20.16155088852988692ABI Solid3 Adapter B (95% over 23bp)

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)