FastQCFastQC Report
Fri 10 Feb 2017
ERR1631226.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631226.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547184
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT146702.6809994444281995No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG144042.6323869118980086No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT134532.4585879704084914RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC81911.4969370449428345No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC50850.9293034884060938RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC42260.7723179040322816RNA PCR Primer, Index 27 (95% over 21bp)
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG19040.3479633907424194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17880.3267639404660955No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16930.309402321705313No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA13720.2507383256820375No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT11860.21674610368724234No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT10600.193719114593994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6670.12189683908886224No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6180.11294189888593233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC16900.033.8254471
CGTCTTC7250.032.15172637
TCGCCGA352.3860373E-531.7142871
TTATACA19100.030.2198942
CGCCGTT250.00549385829.625
TATACAC20400.029.1102963
CCGTCTT22950.025.31154837
GTATCAA6600.024.9469721
GCCGTCT23600.024.61440536
ACCGTTA652.678582E-622.769238
TAGGACC903.8180588E-922.611114
CGTTATA502.6995956E-422.210
GGTATCA2450.021.8979591
GTATAAC450.00382328520.5555551
GCTAAGG450.00382328520.5555551
TGCCGTC28350.020.55555535
CCGTTAT759.251227E-619.7333349
TTAGGAC851.2428682E-619.5882363
ATGCCGT30150.019.3283634
AACCGTT500.00703028718.57
Produced by FastQC (version 0.11.5)