FastQCFastQC Report
Fri 10 Feb 2017
ERR1631225.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631225.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375996
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT100512.6731667358163387No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG90502.4069404993670145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT76642.0383195565910275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70421.8728922648113278No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT62731.6683688124341747Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC51561.3712911839487654No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA31730.8438919562974075No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC25530.678996585070054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24020.6388365833679082No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC23710.6305918148065405Illumina PCR Primer Index 10 (95% over 23bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA23680.6297939339780211No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA18730.4981435972723114No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT18190.48378174235896126No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14580.3877700826604538No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13990.37207842636623795No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT13800.3670251811189481No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG13350.35505696869115627No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC12950.34441855764423024No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG12040.32021617251247353No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11430.3039925956659113No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11270.2997372312471409No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG10500.27925828998180835No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10260.2728752433536527No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9780.2601091500973414No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG9460.25159842125980064No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9420.25053458015510804No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA8810.23431100330854582No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8740.23244928137533377No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8720.23191736082298747No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8450.2247364333663124No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG8310.22101298949988832No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8170.21728954563346417No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8160.21702358535729102No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8150.21675762508111787No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG8110.21569378397642527No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7930.21090649900530858No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT7900.2101086181767891No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7720.20532133320567242No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7100.18883179608293704No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7060.18776795497824444No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC6910.1837785508356472No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6800.18085298779774253No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6740.17925722614070363No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6740.17925722614070363No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6640.1765976233789721No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG6490.17260821923637484No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6470.17207629868402854No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6360.16915073564612387No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6360.16915073564612387No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT6300.16755497398908498No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6230.16569325205587293No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6200.16489537122735348No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6190.16462941095118033No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA5930.15771444377067842No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5580.14840583410461813No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA5540.14734199299992554No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA5490.1460121916190598No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC5380.14308662858115512No Hit
ACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT5370.14282066830498197No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5370.14282066830498197No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG5160.1372355025053458No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC5160.1372355025053458No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5150.13696954222917265No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5130.13643762167682635No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA5080.13510782029596058No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4970.13218225725805594No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4920.13085245587719016No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA4860.12925669422015126No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4780.12712901201076607No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4770.12686305173459292No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4640.12340556814434196No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4640.12340556814434196No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4510.119948084554091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC4470.11888424344939841No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4460.11861828317322526No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC4380.11649060096384004No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG4320.11489483930680114No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA4290.11409695847828169No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4290.11409695847828169No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA4220.11223523654506963No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT4120.10957563378333814No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4070.10824583240247236No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG4040.10744795157395291No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4020.10691603102160661No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3980.10585218991691402No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3940.1047883488122214No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3930.10452238853604826No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT3820.10159682549814361No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3760.10000106384110469No Hit
GGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACT3760.10000106384110469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAAC308.2971055E-637.01
TTACGGG800.037.03
GATTACG750.037.01
TTGAAAG251.2320463E-436.9999962
CTTATAC11250.035.026671
ATTACGG800.034.68752
ACTCAGA603.45608E-1133.9166641
CTAGTTC451.076678E-732.888893
CCTAGTT401.5962778E-632.3752
TTATACA12300.032.0365872
CGTCTTC2950.031.98305137
AGCAACG352.3840656E-531.7142872
TCAGAGT658.731149E-1131.3076923
TTGTGTG303.5949136E-430.8333323
AGGCTGC303.5949136E-430.8333328
TATACAC12850.030.809343
TCCGGAC250.00549169929.5999987
GTTGACA250.00549169929.5999982
ACAAAGG250.00549169929.59999830
GTACTGA250.00549169929.5999986
Produced by FastQC (version 0.11.5)