FastQCFastQC Report
Fri 10 Feb 2017
ERR1631224.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631224.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517883
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG93621.8077442202196248No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT93401.8034961564677738No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT86521.6706476173189697RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC51680.9979087940712478No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC29290.5655717604169281RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC26930.5200016219879781No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG13570.26202829596646343No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12170.2349951630001371No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11590.2237957221998019No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA8970.17320514479139112No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT7670.14810294989408804RNA PCR Primer, Index 26 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT7390.14269632330082277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12500.032.561
CGTCTTC3900.030.83333437
TTATACA14000.029.2035712
TATACAC14550.028.7353953
GATTACG405.933923E-527.7500021
GCCGTCT13850.026.44765336
CCGTCTT14050.026.20284737
ATTACGG603.7211972E-521.5833342
TGCCGTC17150.021.57434535
GTATCAA5150.021.5533961
ATGCCGT17750.020.94929534
GGTATCA2400.020.81251
TTACGGG555.1378505E-420.1818183
TATGCCG19200.019.27083433
GTATTAC852.7184402E-517.4117641
GTATTGG650.001578349617.0769231
GAATTAG650.001578349617.0769231
ATACACA25550.016.7984334
TACACAT26200.016.452295
GTATAGG904.4383705E-516.4444451
Produced by FastQC (version 0.11.5)