##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631224.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	517883
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50405979729012	31.0	31.0	34.0	28.0	34.0
2	31.53431180401751	31.0	31.0	34.0	30.0	34.0
3	31.019861628977974	31.0	31.0	34.0	27.0	34.0
4	35.120291648885946	37.0	35.0	37.0	32.0	37.0
5	35.31338931766442	37.0	35.0	37.0	33.0	37.0
6	35.503057640432296	37.0	35.0	37.0	33.0	37.0
7	35.49763749727255	37.0	35.0	37.0	33.0	37.0
8	35.524303752005764	37.0	35.0	37.0	33.0	37.0
9	37.3017708633031	39.0	37.0	39.0	34.0	39.0
10	37.136204509512766	39.0	37.0	39.0	33.0	39.0
11	37.18568093565535	39.0	37.0	39.0	33.0	39.0
12	37.12061990835768	39.0	37.0	39.0	33.0	39.0
13	37.193068704707436	39.0	37.0	39.0	33.0	39.0
14	38.3494013126517	40.0	38.0	41.0	34.0	41.0
15	38.3550550993178	40.0	38.0	41.0	34.0	41.0
16	38.33751831977493	40.0	38.0	41.0	34.0	41.0
17	38.303560842893084	40.0	38.0	41.0	33.0	41.0
18	38.363124489508245	40.0	38.0	41.0	34.0	41.0
19	38.389893083959116	40.0	38.0	41.0	34.0	41.0
20	38.39179698889517	40.0	38.0	41.0	34.0	41.0
21	38.36911425939836	40.0	38.0	41.0	34.0	41.0
22	38.283527360426966	40.0	38.0	41.0	34.0	41.0
23	38.28458165261266	40.0	38.0	41.0	34.0	41.0
24	38.219258017737594	40.0	38.0	41.0	34.0	41.0
25	38.201031893304084	40.0	38.0	41.0	33.0	41.0
26	38.09621092022716	40.0	37.0	41.0	33.0	41.0
27	38.03933513940407	40.0	37.0	41.0	33.0	41.0
28	38.048387377071656	40.0	37.0	41.0	33.0	41.0
29	37.98471469424561	40.0	37.0	41.0	33.0	41.0
30	37.921955345126214	40.0	37.0	41.0	33.0	41.0
31	37.87060397811861	40.0	37.0	41.0	33.0	41.0
32	37.803073281030656	40.0	37.0	41.0	33.0	41.0
33	37.73989684928835	40.0	37.0	41.0	33.0	41.0
34	37.68135853078784	40.0	37.0	41.0	33.0	41.0
35	37.68448471952159	40.0	37.0	41.0	33.0	41.0
36	37.66341239237434	40.0	37.0	41.0	33.0	41.0
37	37.62714358262387	40.0	37.0	41.0	33.0	41.0
38	37.61272333712441	40.0	37.0	41.0	33.0	41.0
39	37.52396969971982	40.0	37.0	41.0	32.0	41.0
40	37.41624266484901	40.0	37.0	41.0	32.0	41.0
41	37.396423516508555	39.0	37.0	41.0	32.0	41.0
42	37.33510464718865	39.0	37.0	41.0	31.0	41.0
43	36.70709021149565	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	6.0
10	18.0
11	8.0
12	15.0
13	9.0
14	5.0
15	10.0
16	2.0
17	3.0
18	14.0
19	27.0
20	39.0
21	85.0
22	182.0
23	334.0
24	576.0
25	1033.0
26	1709.0
27	2719.0
28	4050.0
29	5883.0
30	8226.0
31	11220.0
32	14423.0
33	18769.0
34	24968.0
35	33588.0
36	46673.0
37	69998.0
38	123725.0
39	149562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.24235589891926	23.685272542253752	15.641950015737146	24.430421543089846
2	13.102959548778392	22.111944203613557	41.306047891125985	23.479048356482064
3	13.965316490404204	29.112753266664477	34.75688524241962	22.1650450005117
4	11.271657884116683	19.732835408769933	37.488776422473805	31.506730284639577
5	12.09056871918947	38.55253020469874	35.937846965434275	13.419054110677509
6	27.333973117480205	39.93064070456068	18.144445753191356	14.590940424767757
7	23.319939059594542	34.3075559537579	22.986659148881117	19.385845837766446
8	24.306262225251647	35.54992150736749	22.601437004110966	17.54237926326989
9	22.978163021377416	15.34342699026614	19.70927796432785	41.969132024028596
10	13.775698372026113	28.991297262122913	34.04533456398453	23.187669801866445
11	30.85561796776492	23.06138645215232	25.38237401111834	20.70062156896442
12	19.670659202947384	26.77651129695317	29.77062386678072	23.782205633318725
13	28.537719909709335	20.956857050723812	26.5548396066293	23.950583432937556
14	21.251904387670574	22.906911406630456	26.153783769693156	29.687400436005817
15	23.39524564428645	29.741852889552273	22.400426351125642	24.462475115035637
16	24.58972393378427	27.928702042739385	23.77950232002209	23.702071703454255
17	20.095851765746318	31.520246851122746	25.24971856577644	23.1341828173545
18	22.915021346520355	25.821469328014242	26.687108864357395	24.576400461108012
19	22.206367075188798	28.302531652902296	27.34440018305293	22.146701088855977
20	21.463921387649336	26.455203202267697	25.55751009397876	26.52336531610422
21	23.67754878997766	25.71758485990079	26.689232896233317	23.915633453888233
22	20.788093063491175	30.182492956903396	27.501385448064525	21.528028531540908
23	24.28135312416125	26.079249560228856	26.106475787002083	23.532921528607815
24	22.91000090754089	29.505699163710723	26.784814330649976	20.799485598098414
25	23.077027050511408	27.495592633857452	25.95547643000446	23.471903885626677
26	22.587920437627805	26.891595205866963	27.617627919819725	22.902856436685507
27	21.123497006080523	29.219341048074565	27.12176302369454	22.53539892215037
28	21.471838233732328	25.656953404533457	27.37741922403323	25.493789137700983
29	21.015364474215218	26.83868750277572	29.954835358565546	22.191112664443512
30	23.453559973970954	25.260724912769874	28.013277130162606	23.27243798309657
31	21.545020786548314	27.264652440802266	25.906044415437464	25.284282357211957
32	23.920846986674597	26.645786789680294	29.113332548085186	20.320033675559923
33	22.430742078809306	26.183520215956115	28.622681184746362	22.763056520488217
34	21.139909979667223	30.651517813869155	26.453465358005573	21.75510684845805
35	22.898801466740558	25.581260632227742	30.933820959560364	20.586116941471335
36	22.886057275485	25.96513112034958	25.515994925494756	25.632816678670668
37	21.352892448680493	27.305974515479363	28.43055284687854	22.9105801889616
38	21.58943236213585	25.517539675949973	28.520148373281224	24.372879588632955
39	24.031875925643437	26.195105844370254	26.899898239563765	22.87311999042255
40	20.655630711956174	25.34491381257929	32.3694734138792	21.62998206158534
41	22.09977929377871	26.65814479332204	26.41697062850103	24.825105284398212
42	22.134922366634935	25.244891220603883	30.587410669977583	22.032775742783603
43	22.6373524521948	24.97494607855442	29.799394844009168	22.588306625241607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	8.5
2	12.0
3	47.0
4	82.0
5	82.0
6	107.5
7	133.0
8	142.0
9	151.0
10	204.5
11	258.0
12	258.0
13	408.0
14	558.0
15	1323.0
16	2088.0
17	2567.5
18	3047.0
19	3047.0
20	3456.0
21	3865.0
22	4773.5
23	5682.0
24	7445.5
25	9209.0
26	9209.0
27	11476.5
28	13744.0
29	16296.5
30	18849.0
31	21252.5
32	23656.0
33	23656.0
34	25904.0
35	28152.0
36	30063.0
37	31974.0
38	34084.5
39	36195.0
40	36195.0
41	37565.0
42	38935.0
43	39022.5
44	39110.0
45	38461.0
46	37812.0
47	37812.0
48	36350.0
49	34888.0
50	34633.5
51	34379.0
52	41199.0
53	48019.0
54	48019.0
55	36415.0
56	24811.0
57	29077.0
58	33343.0
59	27252.5
60	21162.0
61	21162.0
62	15771.5
63	10381.0
64	8676.0
65	6971.0
66	5663.0
67	4355.0
68	4355.0
69	3496.5
70	2638.0
71	2093.0
72	1548.0
73	1216.0
74	884.0
75	884.0
76	664.0
77	444.0
78	359.5
79	275.0
80	202.5
81	130.0
82	130.0
83	107.0
84	84.0
85	57.5
86	31.0
87	23.0
88	15.0
89	15.0
90	11.0
91	7.0
92	4.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	517883.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.50026688146306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.17551124490281	51.768164064451184
2	9.754403860997272	11.997968814418119
3	2.533323748358403	4.674002598635704
4	1.0712132478525704	2.6351960251956763
5	0.5595545770629844	1.7206377912058861
6	0.35412905306302467	1.3067418764313485
7	0.2566314198744883	1.1048030568714882
8	0.18432505127100104	0.906883187688474
9	0.13676968947511026	0.7570235163612699
>10	0.8888347604635579	10.211986087229112
>50	0.06554558657029823	2.77063806658722
>100	0.0159943772307444	1.605484919622691
>500	9.408457194555508E-4	0.46347672535479595
>1k	0.0015680761990925847	1.8043382295855663
>5k	0.0012544609592740677	6.272655040361495
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG	9362	1.8077442202196248	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT	9340	1.8034961564677738	No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	8652	1.6706476173189697	RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC	5168	0.9979087940712478	No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC	2929	0.5655717604169281	RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	2693	0.5200016219879781	No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG	1357	0.26202829596646343	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1217	0.2349951630001371	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1159	0.2237957221998019	No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA	897	0.17320514479139112	No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT	767	0.14810294989408804	RNA PCR Primer, Index 26 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT	739	0.14269632330082277	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.9309380690233122E-4	0.0
9	0.0	0.0	0.0	3.8618761380466245E-4	0.0
10	0.0	0.0	0.0	3.8618761380466245E-4	0.0
11	0.0	0.0	0.0	3.8618761380466245E-4	0.0
12	0.0	0.0	0.0	3.8618761380466245E-4	0.0
13	0.0	0.0	0.0	3.8618761380466245E-4	0.0
14	0.0	0.0	0.0	3.8618761380466245E-4	0.0
15	0.0	0.0	0.0	3.8618761380466245E-4	0.0
16	0.0	0.0	0.0	3.8618761380466245E-4	0.0
17	0.0	0.0	0.0	3.8618761380466245E-4	0.0
18	0.0	0.0	0.0	3.8618761380466245E-4	0.0
19	0.0	0.0	0.0	5.792814207069936E-4	0.0
20	0.0	0.0	0.0	7.723752276093249E-4	0.0
21	0.0	0.0	0.0	0.0011585628414139872	1.9309380690233122E-4
22	0.0	0.0	0.0	0.0013516566483163185	1.9309380690233122E-4
23	0.0	0.0	0.0	0.0013516566483163185	1.9309380690233122E-4
24	0.0	0.0	0.0	0.0019309380690233121	1.9309380690233122E-4
25	0.0	0.0	0.0	0.0021240318759256434	1.9309380690233122E-4
26	0.0	0.0	0.0	0.002510219489730306	1.9309380690233122E-4
27	0.0	0.0	0.0	0.0030895009104372996	1.9309380690233122E-4
28	0.0	0.0	0.0	0.006179001820874599	1.9309380690233122E-4
29	0.0	0.0	0.0	0.012358003641749198	1.9309380690233122E-4
30	0.0	0.0	0.0	0.020854131145451774	1.9309380690233122E-4
31	0.0	0.0	0.0	0.05020438979460612	1.9309380690233122E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1250	0.0	32.56	1
CGTCTTC	390	0.0	30.833334	37
TTATACA	1400	0.0	29.203571	2
TATACAC	1455	0.0	28.735395	3
GATTACG	40	5.933923E-5	27.750002	1
GCCGTCT	1385	0.0	26.447653	36
CCGTCTT	1405	0.0	26.202847	37
ATTACGG	60	3.7211972E-5	21.583334	2
TGCCGTC	1715	0.0	21.574345	35
GTATCAA	515	0.0	21.553396	1
ATGCCGT	1775	0.0	20.949295	34
GGTATCA	240	0.0	20.8125	1
TTACGGG	55	5.1378505E-4	20.181818	3
TATGCCG	1920	0.0	19.270834	33
GTATTAC	85	2.7184402E-5	17.411764	1
GTATTGG	65	0.0015783496	17.076923	1
GAATTAG	65	0.0015783496	17.076923	1
ATACACA	2555	0.0	16.798433	4
TACACAT	2620	0.0	16.45229	5
GTATAGG	90	4.4383705E-5	16.444445	1
>>END_MODULE