FastQCFastQC Report
Fri 10 Feb 2017
ERR1631223.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631223.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136596
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG85576.264458695715834No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT73415.3742422911359045TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC49783.644323406249085No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT43973.218981522152918No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC26621.948812556736654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26371.9305104102609154No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC26221.9195291223754722RNA PCR Primer, Index 12 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22241.6281589504817124No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG11920.87264634396322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8330.6098275205716127No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7630.5585815104395444No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT7010.5131921871797124No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA6820.499282555858151No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT6320.46267826290667374No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6260.4582857477524964No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5210.38141673255439396No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4800.35140121233418253No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3500.25623005066034144No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3380.2474450203519869No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.23353538903042548No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3180.23280330317139594No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3150.2306070455943073No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3140.22987495973527777No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC3000.21962575770886408No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG2920.21376907083662774No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2660.1947348385018595No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2560.18741397991156403No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2400.17570060616709127No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2300.1683797475767958No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2290.16764766171776627No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG2250.16471931828164807No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG2240.16398723242261853No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2230.16325514656358897No Hit
ATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT2220.1625230607045594TruSeq Adapter, Index 12 (95% over 21bp)
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2210.1617909748455299No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG2160.15813054555038217No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2120.15520220211426397No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2100.15373803039620487No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.15373803039620487No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2020.14788134352396848No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA1970.14422091422882075No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG1880.13763214149755482No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT1880.13763214149755482No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG1830.1339717122024071No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1780.13031128290725938No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT1770.12957919704822982No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1720.12591876775308208No Hit
TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT1700.12445459603502299TruSeq Adapter, Index 12 (95% over 24bp)
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA1690.12372251017599345No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG1680.12299042431696389No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1660.1215262525989048No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC1650.12079416673987525No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG1630.11932999502181617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC1570.11493747986763889No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA1550.11347330814957979No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1540.11274122229055024No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1520.11127705057249115No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA1470.1076166212773434No Hit
CTTTACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA1470.1076166212773434No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC1420.10395619198219568No Hit
TCGCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT1410.10322410612316611TruSeq Adapter, Index 12 (95% over 22bp)
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA1400.10249202026413659No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC1400.10249202026413659No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG1390.10175993440510703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACGC355.582988E-737.03
TAGCAAC355.582988E-737.01
AGCAACG355.582988E-737.02
GGATCAA251.2279372E-436.9999962
CTTATAC5700.036.0263141
TTATACA6250.032.8562
CGTCTTC3550.032.83098637
ACGTGTG303.583036E-430.8333349
GTAGCAC303.583036E-430.8333344
GGTATCA1950.030.3589731
TTGTAGC250.005479607729.5999982
TATACAC7300.028.1301363
GATCAAC405.9024595E-527.7500023
GTATCAA6100.026.6885241
GCACGTG358.829261E-426.4285727
TCCATCA358.829261E-426.42857233
GGGATCA358.829261E-426.4285721
CCGTCTT12000.025.74583437
GCCGTCT12300.024.9674836
GGGAACC451.3148887E-424.66666618
Produced by FastQC (version 0.11.5)