##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1631223.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 136596 Sequences flagged as poor quality 0 Sequence length 43 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.578669946411313 31.0 30.0 33.0 27.0 34.0 2 30.55523587806378 31.0 30.0 34.0 27.0 34.0 3 29.95295616269876 31.0 30.0 34.0 25.0 34.0 4 34.18818999092213 35.0 35.0 37.0 30.0 37.0 5 34.271347623649305 35.0 35.0 37.0 30.0 37.0 6 34.54261471785411 35.0 35.0 37.0 31.0 37.0 7 34.542409733813585 35.0 35.0 37.0 31.0 37.0 8 34.596935488594106 35.0 35.0 37.0 31.0 37.0 9 36.26065184924888 37.0 35.0 39.0 32.0 39.0 10 36.01816305016252 37.0 35.0 39.0 31.0 39.0 11 36.08347242964655 37.0 35.0 39.0 31.0 39.0 12 35.90262525989048 37.0 35.0 39.0 30.0 39.0 13 36.09018565697385 37.0 35.0 39.0 31.0 39.0 14 36.660546428885176 38.0 36.0 40.0 31.0 41.0 15 36.69980087264634 38.0 36.0 40.0 31.0 41.0 16 36.72535798998506 38.0 36.0 40.0 31.0 41.0 17 36.699581246888634 38.0 36.0 40.0 31.0 41.0 18 36.91906058742569 38.0 36.0 40.0 31.0 41.0 19 36.84040528273156 38.0 36.0 40.0 31.0 41.0 20 36.85860493718703 38.0 36.0 40.0 31.0 41.0 21 36.68453688248558 38.0 36.0 40.0 31.0 41.0 22 36.28869073764971 38.0 35.0 40.0 30.0 41.0 23 36.45087703885912 38.0 35.0 40.0 30.0 41.0 24 36.13803478872002 38.0 35.0 40.0 30.0 41.0 25 36.00177896863744 38.0 34.0 40.0 30.0 41.0 26 35.88696594336584 38.0 34.0 40.0 29.0 41.0 27 35.5222554101145 38.0 34.0 40.0 27.0 41.0 28 35.583384579343466 38.0 34.0 40.0 29.0 41.0 29 35.26818501273829 38.0 33.0 40.0 27.0 41.0 30 34.97679287826876 37.0 33.0 40.0 26.0 40.0 31 34.92702568157193 37.0 33.0 40.0 27.0 40.0 32 34.368883422647805 37.0 33.0 39.0 25.0 40.0 33 34.368627192597145 37.0 33.0 39.0 25.0 40.0 34 34.33646666080998 37.0 33.0 39.0 25.0 40.0 35 34.17464640253009 37.0 33.0 39.0 24.0 40.0 36 34.17995402500805 37.0 32.0 39.0 24.0 40.0 37 34.20097953087938 37.0 33.0 39.0 24.0 40.0 38 34.02850010249202 37.0 32.0 39.0 23.0 40.0 39 33.691813815924334 36.0 32.0 39.0 23.0 40.0 40 33.22440627836833 36.0 31.0 39.0 21.0 40.0 41 33.15803537438871 36.0 31.0 39.0 21.0 40.0 42 32.958161293156465 36.0 31.0 39.0 20.0 40.0 43 31.863561158452665 35.0 29.0 38.0 17.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 1.0 9 2.0 10 6.0 11 16.0 12 7.0 13 4.0 14 6.0 15 8.0 16 6.0 17 5.0 18 26.0 19 43.0 20 91.0 21 158.0 22 284.0 23 499.0 24 756.0 25 1161.0 26 1733.0 27 2410.0 28 3216.0 29 4105.0 30 5195.0 31 6495.0 32 8129.0 33 9680.0 34 12159.0 35 15246.0 36 19289.0 37 21664.0 38 18619.0 39 5576.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.995080383027318 25.654484757972412 19.323406249084893 23.02702860991537 2 12.758060265307916 22.67269905414507 39.93674778177984 24.63249289876717 3 14.864271281735922 32.3215906761545 35.11449822835222 17.699639813757358 4 9.529561626987613 18.724560016398723 27.779730006735193 43.96614834987847 5 12.521596532841372 39.714193680634864 28.10916864329849 19.655041143225276 6 25.535886848809632 35.74482415297666 15.963132156139274 22.75615684207444 7 20.52402705789335 36.3056019209933 15.666637383232304 27.50373363788105 8 29.110662098450906 25.656681015549506 25.268675510263844 19.963981375735745 9 20.45667515886263 11.387595537204604 14.599256200767227 53.55647310316554 10 16.07880172186594 24.87481331810595 27.742393627924685 31.303991332103433 11 30.77615742774313 19.912003279744646 19.838794693841695 29.473044598670533 12 18.82339160736771 29.34932208849454 21.184368502738003 30.64291780139975 13 37.580895487422765 16.200327974464845 21.29271720987437 24.926059328238015 14 24.013880347887202 25.799437758060268 23.06143664528976 27.125245248762774 15 28.773170517438285 26.071041611760226 18.94491785996662 26.21087001083487 16 27.13549445078919 22.111189200269408 18.154265132214707 32.5990512167267 17 18.24870420802952 32.72496998447978 19.48885765322557 29.537468154265135 18 26.609124718146944 15.872353509619607 22.5050513924273 35.013470379806144 19 30.877185276289204 25.37043544466895 22.336671644850508 21.41570763419134 20 22.518961023748865 19.234823860142317 23.144162347360098 35.10205276874872 21 37.63433775513192 17.527599636885412 24.961931535330464 19.8761310726522 22 17.998330844241412 33.73085595478638 28.562329790037776 19.708483410934434 23 29.937187033295267 23.000673518990308 23.403320741456557 23.65881870625787 24 31.28276084219157 23.64783741837243 27.15013616796978 17.919265571466223 25 18.62133591027556 26.270901051275292 29.461331224926056 25.64643181352309 26 34.35239684910246 25.986119652112798 23.754721953790742 15.906761544993998 27 18.473454566751588 27.500073208585903 31.367682801839 22.658789422823506 28 24.46630940876746 23.887961580134117 29.306861108670823 22.338867902427598 29 25.349937040616123 19.76485402207971 31.57705935752145 23.308149579782718 30 17.063457202260683 25.60177457612229 35.62915458724999 21.705613634367037 31 32.20372485285074 20.791238396439134 23.292775776743095 23.712260973967027 32 21.610442472693197 21.647778851503706 39.10436616006325 17.637412515739843 33 17.123488242701104 29.901314826202817 33.99221060645993 18.98298632463615 34 20.940583911681163 30.208790886995228 26.869747283961466 21.98087791736215 35 18.401710152566693 19.536443234062492 44.9405546282468 17.121291985124014 36 23.242261852470058 23.5951272365223 24.214471873261296 28.948139037746344 37 21.796392280886703 22.030659755776156 31.81498726170605 24.357960701631086 38 21.1074994875399 17.80652434917567 29.776128144308768 31.309848018975668 39 30.17950745263405 19.976426835339247 26.585697970658 23.25836774136871 40 18.42074438490146 18.140355500893147 41.656417464640256 21.78248264956514 41 24.704237312952063 22.481624644938357 24.791355530176578 28.022782511933 42 20.698995578201412 17.99759875838238 38.80421095786114 22.499194705555066 43 25.03294386365633 17.85557410173065 31.571202670649214 25.54027936396381 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 2.5 2 4.0 3 30.5 4 57.0 5 57.0 6 83.5 7 110.0 8 137.5 9 165.0 10 236.0 11 307.0 12 307.0 13 575.5 14 844.0 15 2298.5 16 3753.0 17 3664.0 18 3575.0 19 3575.0 20 2867.0 21 2159.0 22 1666.0 23 1173.0 24 1106.0 25 1039.0 26 1039.0 27 1081.0 28 1123.0 29 1061.0 30 999.0 31 1087.0 32 1175.0 33 1175.0 34 1396.5 35 1618.0 36 1615.0 37 1612.0 38 1738.5 39 1865.0 40 1865.0 41 2348.5 42 2832.0 43 3677.5 44 4523.0 45 5893.0 46 7263.0 47 7263.0 48 10116.0 49 12969.0 50 18663.5 51 24358.0 52 18451.0 53 12544.0 54 12544.0 55 18197.5 56 23851.0 57 19452.5 58 15054.0 59 9988.5 60 4923.0 61 4923.0 62 4026.5 63 3130.0 64 2439.5 65 1749.0 66 1376.0 67 1003.0 68 1003.0 69 765.0 70 527.0 71 366.0 72 205.0 73 128.0 74 51.0 75 51.0 76 31.0 77 11.0 78 7.5 79 4.0 80 6.0 81 8.0 82 8.0 83 7.5 84 7.0 85 5.5 86 4.0 87 2.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 136596.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.829423994846113 #Duplication Level Percentage of deduplicated Percentage of total 1 74.48401934190353 18.49395297080442 2 10.464087746196485 5.196345427391725 3 4.177969100129732 3.1120969867346044 4 2.1169949286472463 2.1025505871328587 5 1.6511381059087158 2.0498404052827315 6 1.0378582379997643 1.5461653342704031 7 0.8108267484373157 1.409265278631878 8 0.6191767897157684 1.229904243169639 9 0.4599599009317137 1.0278485460774838 >10 3.532256162283288 17.449266449969254 >50 0.38035145653968633 6.413804210957862 >100 0.21818610685222314 9.684031743242848 >500 0.020639226323858947 3.4832645172625845 >1k 0.020639226323858947 15.162962312219976 >5k 0.005896921806816842 11.638700986851738 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG 8557 6.264458695715834 No Hit TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT 7341 5.3742422911359045 TruSeq Adapter, Index 12 (95% over 22bp) ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC 4978 3.644323406249085 No Hit CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT 4397 3.218981522152918 No Hit CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC 2662 1.948812556736654 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 2637 1.9305104102609154 No Hit CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC 2622 1.9195291223754722 RNA PCR Primer, Index 12 (95% over 23bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 2224 1.6281589504817124 No Hit CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG 1192 0.87264634396322 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 833 0.6098275205716127 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA 763 0.5585815104395444 No Hit TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT 701 0.5131921871797124 No Hit TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA 682 0.499282555858151 No Hit ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT 632 0.46267826290667374 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA 626 0.4582857477524964 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA 521 0.38141673255439396 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT 480 0.35140121233418253 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 350 0.25623005066034144 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC 338 0.2474450203519869 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 319 0.23353538903042548 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG 318 0.23280330317139594 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG 315 0.2306070455943073 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT 314 0.22987495973527777 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC 300 0.21962575770886408 No Hit GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG 292 0.21376907083662774 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG 266 0.1947348385018595 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC 256 0.18741397991156403 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG 240 0.17570060616709127 No Hit GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC 230 0.1683797475767958 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG 229 0.16764766171776627 No Hit CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG 225 0.16471931828164807 No Hit GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG 224 0.16398723242261853 No Hit GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 223 0.16325514656358897 No Hit ATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT 222 0.1625230607045594 TruSeq Adapter, Index 12 (95% over 21bp) GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA 221 0.1617909748455299 No Hit GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG 216 0.15813054555038217 No Hit GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG 212 0.15520220211426397 No Hit GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG 210 0.15373803039620487 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 210 0.15373803039620487 No Hit GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG 202 0.14788134352396848 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA 197 0.14422091422882075 No Hit GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG 188 0.13763214149755482 No Hit CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT 188 0.13763214149755482 No Hit ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG 183 0.1339717122024071 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC 178 0.13031128290725938 No Hit AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT 177 0.12957919704822982 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA 172 0.12591876775308208 No Hit TCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT 170 0.12445459603502299 TruSeq Adapter, Index 12 (95% over 24bp) TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA 169 0.12372251017599345 No Hit GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG 168 0.12299042431696389 No Hit GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC 166 0.1215262525989048 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC 165 0.12079416673987525 No Hit TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG 163 0.11932999502181617 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC 157 0.11493747986763889 No Hit GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA 155 0.11347330814957979 No Hit ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA 154 0.11274122229055024 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT 152 0.11127705057249115 No Hit GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA 147 0.1076166212773434 No Hit CTTTACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA 147 0.1076166212773434 No Hit ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC 142 0.10395619198219568 No Hit TCGCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT 141 0.10322410612316611 TruSeq Adapter, Index 12 (95% over 22bp) GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA 140 0.10249202026413659 No Hit GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC 140 0.10249202026413659 No Hit CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG 139 0.10175993440510703 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 7.32085859029547E-4 0.0 12 0.0 0.0 0.0 7.32085859029547E-4 0.0 13 0.0 0.0 0.0 7.32085859029547E-4 0.0 14 0.0 0.0 0.0 7.32085859029547E-4 0.0 15 0.0 0.0 0.0 7.32085859029547E-4 0.0 16 0.0 0.0 0.0 7.32085859029547E-4 0.0 17 0.0 0.0 0.0 7.32085859029547E-4 0.0 18 0.0 0.0 0.0 7.32085859029547E-4 0.0 19 0.0 0.0 0.0 7.32085859029547E-4 0.0 20 0.0 0.0 0.0 7.32085859029547E-4 0.0 21 0.0 0.0 0.0 7.32085859029547E-4 0.0 22 0.0 0.0 0.0 0.001464171718059094 0.0 23 0.0 0.0 0.0 0.002196257577088641 0.0 24 0.0 0.0 0.0 0.005856686872236376 0.0 25 0.0 0.0 0.0 0.005856686872236376 0.0 26 0.0 0.0 0.0 0.0065887727312659225 0.0 27 0.0 0.0 0.0 0.008052944449325018 0.0 28 0.0 0.0 0.0 0.016105888898650035 0.0 29 0.0 0.0 0.0 0.04392515154177282 0.0 30 0.0 0.0 0.0 0.06881607074877742 0.0 31 0.0 0.0 0.0 0.10468827784122522 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAACGC 35 5.582988E-7 37.0 3 TAGCAAC 35 5.582988E-7 37.0 1 AGCAACG 35 5.582988E-7 37.0 2 GGATCAA 25 1.2279372E-4 36.999996 2 CTTATAC 570 0.0 36.026314 1 TTATACA 625 0.0 32.856 2 CGTCTTC 355 0.0 32.830986 37 ACGTGTG 30 3.583036E-4 30.833334 9 GTAGCAC 30 3.583036E-4 30.833334 4 GGTATCA 195 0.0 30.358973 1 TTGTAGC 25 0.0054796077 29.599998 2 TATACAC 730 0.0 28.130136 3 GATCAAC 40 5.9024595E-5 27.750002 3 GTATCAA 610 0.0 26.688524 1 GCACGTG 35 8.829261E-4 26.428572 7 TCCATCA 35 8.829261E-4 26.428572 33 GGGATCA 35 8.829261E-4 26.428572 1 CCGTCTT 1200 0.0 25.745834 37 GCCGTCT 1230 0.0 24.96748 36 GGGAACC 45 1.3148887E-4 24.666666 18 >>END_MODULE