FastQCFastQC Report
Fri 10 Feb 2017
ERR1631222.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631222.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192045
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG127486.638027545627327No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT126376.580228592257023No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT125396.529198885677837RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC75473.9298081178890367No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC44782.331745163893879RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC40672.1177328230362678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29681.5454711135410972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26651.3876955921789165No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG17930.9336353458824754No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14840.7727355567705486No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA12010.6253742612408549No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10460.5446640110390794No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT10330.5378947642479627No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT9580.4988414173761358No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8880.4623916269624307No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8020.4176104558827358No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7630.39730271550938584No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6230.32440313468197557No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6000.3124267749746153No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5980.3113853523913666No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5590.29107761201801663No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5270.27441485068603716No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5070.2640006248535499No Hit
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT4200.2186987424822307No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4090.2129709182743628No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3980.20724309406649483No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3850.20047384727537818No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3800.19787029081725638No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3740.1947460230675102No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3660.19058033273451536No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3520.18329037465177433No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3490.18172824077690125No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3430.1786039730271551No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3370.17547970527740894No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3300.17183472623603843No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3250.16923116977791663No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3250.16923116977791663No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.16871045848629226No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2920.1520476971543128No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2900.15100627457106408No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2830.14736129552969354No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2830.14736129552969354No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2790.14527845036319612No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2790.14527845036319612No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2780.14475773907157177No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2740.14267489390507432No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2670.13902991486370384No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2610.13590564711395767No Hit
CTTTACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA2550.1327813793642115No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2520.13121924548933844No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2450.12757426644796793No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2420.12601213257309485No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2410.12549142128147048No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2340.12184644224009998No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2290.11924288578197817No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2260.11768075190710511No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2250.11716004061548076No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.10934937124111535No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2100.10934937124111535No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2090.10882865994949101No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA2070.1077872373662423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2040.10622510349136921No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2040.10622510349136921No Hit
TCGCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT2030.10570439219974485RNA PCR Primer, Index 17 (95% over 21bp)
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG1990.10362154703324741No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA1960.10205941315837433No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG1950.10153870186674999No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1940.10101799057512563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCAGA308.275949E-637.01
TGCTTGA355.598795E-737.025
CAGACAC700.037.018
TACTATA200.001838120737.02
TCGCCGA251.2297995E-436.9999961
CTTATAC15000.036.2600021
ACAGACA750.034.53333317
TTATACA16250.033.4707682
CTGCTTG451.0720214E-732.8888924
CGTCTTC4850.032.80412337
GACAGAC800.032.37516
TCAGAGT401.5908954E-632.3753
CTTGAAA401.5908954E-632.37527
CTCAGAG352.3780362E-531.7142852
TTTACAC352.3780362E-531.7142852
CGCCGAG303.5884196E-430.8333322
TATACAC17850.030.7815113
AGACAGA850.030.47058715
GGTATCA3150.029.952381
ATAAGAG900.028.77777710
Produced by FastQC (version 0.11.5)