FastQCFastQC Report
Fri 10 Feb 2017
ERR1631221.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631221.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620569
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT120551.9425720588685544No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG108431.7472674271515336No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT91481.4741309991314422TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC61800.995860250834315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60860.9807128619057671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59810.9637929061877083No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC33810.5448225741214918RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC32120.5175895025371876TruSeq Adapter, Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21900.3529019335480825No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG15860.25557190256039214No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14820.2388130892777435No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10770.17355040293665974No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA10520.16952184205140766No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC9730.15679158965401108No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9330.15034589223760775No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA8610.13874363688808175No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC8580.1382602095818515No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8410.1355207881798801No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT8020.12923623319888683No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT7830.12617452692609524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7220.11634483836608016No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT7060.11376655939951882No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC7030.1132831320932886No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG7010.11296084722246841No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6910.11134942286836758No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA6780.1092545712080365No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6660.1073208619831155No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG6420.10345344353327349No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6320.10184201917917267No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6300.1015197343083525No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG200.001841283237.030
GATTACG651.8189894E-1234.1538471
CGTCTTC3500.032.2428637
CTTATAC14650.031.0648461
TTATACA14800.030.6252
TATACAC16050.028.585673
CCGTCTT13750.027.17818337
GGTATCA9550.027.1204191
GCCGTCT14000.026.56071536
TTACGGG1100.025.2272723
GGCTTGG451.3222542E-424.66666622
CGGGCAC451.3222542E-424.66666617
GTATCAA26750.023.5140191
TCTATAC1107.4578566E-1121.8636363
CGGCGTA806.9495036E-720.812512
GGTCACC806.9495036E-720.812531
TGCCGTC17900.020.77374335
TGGGCCA450.00382385520.55555529
CGGGTAT555.1394297E-420.18181816
TTTGGCG555.1394297E-420.18181822
Produced by FastQC (version 0.11.5)