##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631221.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620569
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.415436478457675	31.0	31.0	34.0	28.0	34.0
2	31.39498589197978	31.0	31.0	34.0	28.0	34.0
3	30.846405476264525	31.0	31.0	34.0	27.0	34.0
4	35.01188908888456	37.0	35.0	37.0	32.0	37.0
5	35.236797197410766	37.0	35.0	37.0	33.0	37.0
6	35.45103445386412	37.0	35.0	37.0	33.0	37.0
7	35.460835136785754	37.0	35.0	37.0	33.0	37.0
8	35.48665982348458	37.0	35.0	37.0	33.0	37.0
9	37.2678348418951	39.0	37.0	39.0	34.0	39.0
10	37.09325796164488	39.0	37.0	39.0	33.0	39.0
11	37.153230341831446	39.0	37.0	39.0	33.0	39.0
12	37.068176141573296	39.0	37.0	39.0	33.0	39.0
13	37.15346560978715	39.0	37.0	39.0	33.0	39.0
14	38.203036245768	40.0	38.0	41.0	33.0	41.0
15	38.18061649872939	40.0	37.0	41.0	33.0	41.0
16	38.21351856119142	40.0	38.0	41.0	33.0	41.0
17	38.214124456748564	40.0	38.0	41.0	33.0	41.0
18	38.28969542468283	40.0	38.0	41.0	33.0	41.0
19	38.316493411691525	40.0	38.0	41.0	34.0	41.0
20	38.266900215769724	40.0	38.0	41.0	34.0	41.0
21	38.18719433294283	40.0	37.0	41.0	33.0	41.0
22	38.06360453068071	40.0	37.0	41.0	33.0	41.0
23	38.0950933739842	40.0	37.0	41.0	33.0	41.0
24	38.05673502865918	40.0	37.0	41.0	33.0	41.0
25	38.00420742898856	40.0	37.0	41.0	33.0	41.0
26	37.88993649376621	40.0	37.0	41.0	33.0	41.0
27	37.76874287951863	40.0	37.0	41.0	33.0	41.0
28	37.76026839884042	40.0	37.0	41.0	33.0	41.0
29	37.65685040664294	40.0	37.0	41.0	32.0	41.0
30	37.52204347945192	39.0	37.0	41.0	32.0	41.0
31	37.44111130269156	39.0	36.0	41.0	32.0	41.0
32	37.26838756044856	39.0	36.0	41.0	32.0	41.0
33	37.264889158175805	39.0	36.0	41.0	32.0	41.0
34	37.24115932313732	39.0	36.0	41.0	32.0	41.0
35	37.185083367038956	39.0	36.0	41.0	31.0	41.0
36	37.133440439338735	39.0	36.0	41.0	31.0	41.0
37	37.050664793117285	39.0	36.0	41.0	31.0	41.0
38	36.969877644548795	39.0	35.0	41.0	31.0	41.0
39	36.842004676353476	39.0	35.0	41.0	31.0	41.0
40	36.69365372746624	39.0	35.0	41.0	30.0	41.0
41	36.62197918362019	39.0	35.0	40.0	30.0	41.0
42	36.48989556358761	39.0	35.0	40.0	30.0	41.0
43	35.787643275767884	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	7.0
10	7.0
11	11.0
12	7.0
13	11.0
14	3.0
15	9.0
16	3.0
17	8.0
18	24.0
19	33.0
20	69.0
21	143.0
22	306.0
23	573.0
24	1021.0
25	1745.0
26	2678.0
27	4158.0
28	6244.0
29	8668.0
30	11688.0
31	14779.0
32	19116.0
33	25256.0
34	32992.0
35	44026.0
36	60596.0
37	90323.0
38	144520.0
39	151543.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.78505049398214	22.39025797292485	15.751511918900235	23.07317961419278
2	14.062416910931741	22.873362994284278	40.80352063992884	22.26069945485514
3	14.479775818643859	30.259326521305447	33.73226828926356	21.528629370787133
4	10.67568634591802	19.073946652185334	37.953394384830695	32.29697261706595
5	12.488216459410637	38.45857591984131	34.24518466117386	14.808022959574195
6	26.430743398397276	41.32900612180112	16.439912402972112	15.80033807682949
7	24.02069713440407	32.980055400769295	21.926651186250037	21.072596278576597
8	25.35205593576218	33.066588888584505	22.13887577368512	19.442479401968193
9	24.24774682589688	13.619114071118602	19.092478032257493	43.040661070727026
10	15.673680122597164	27.719721739242537	31.92231645473751	24.684281683422792
11	31.54153687986348	23.74868870343185	23.41592957431003	21.29384484239464
12	21.17250458853085	28.171887413003226	27.28818229721433	23.367425701251594
13	30.2122729301657	21.13673096786981	25.51352065604308	23.137475445921403
14	22.31999987108605	22.068456529410913	27.405332847757464	28.206210751745576
15	24.81110078009053	27.990924458037703	23.80492741338997	23.393047348481797
16	24.07661355949137	26.657631947454675	23.47265171157438	25.793102781479575
17	20.438661937673327	29.566091764171272	24.7690426044485	25.226203693706907
18	24.46142169525065	24.05872674916085	26.791702453715864	24.688149101872636
19	26.988940794657807	24.861538362373885	26.80137099984047	21.34814984312784
20	24.821736180827596	24.190863546197118	26.84214003599922	24.145260236976064
21	25.205577461974414	26.30182945006921	26.575932732701762	21.916660355254614
22	22.416201905025872	29.08701530369709	27.27754689647727	21.219235894799773
23	22.67950864448595	24.895861701116235	28.246496360598094	24.17813329379972
24	24.200048665015494	26.943498627872163	27.683786976146084	21.17266573096626
25	22.306141621640784	28.436805576817402	27.016335008677522	22.240717792864288
26	25.115659983015586	26.542898533442695	27.023908703141796	21.317532780399922
27	23.216435239272347	28.334157845461185	27.55793473409081	20.891472181175665
28	19.930902123696157	26.60783893491296	29.602026527267718	23.85923241412317
29	25.49289442431059	24.56100772033408	28.139175498614982	21.806922356740348
30	23.15230054997913	25.39653124793536	28.846268505194427	22.604899696891078
31	22.51079251461159	26.27701351501606	28.10243502334148	23.10975894703087
32	22.582339755933667	27.07644113708548	28.901056933233853	21.440162173746995
33	19.49162784476827	24.96499180590716	30.63059869249028	24.912781656834294
34	20.431249385644463	26.89789531865111	30.780461157421655	21.89039413828277
35	20.88663790811336	26.874368523081237	31.451135973598422	20.78785759520698
36	23.027254020100905	25.686104204367282	25.827264977786513	25.459376797745293
37	21.50880885123169	26.175010353401472	29.39383050071789	22.922350294648943
38	21.430654770057803	24.78902426643935	29.315837561979407	24.46448340152344
39	23.41786328353495	24.90665824428871	28.221196998238714	23.454281473937627
40	20.59529238489193	24.87346290259423	33.221930196319825	21.309314516194007
41	22.756373586176558	25.444712836122978	26.514215179939697	25.284698397760764
42	21.58148408960164	25.25101962876006	31.131107096874	22.036389184764303
43	22.7666867020428	24.049380487907065	30.076429857115	23.10750295293513
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	35.5
2	53.0
3	167.0
4	281.0
5	281.0
6	411.5
7	542.0
8	560.0
9	578.0
10	875.0
11	1172.0
12	1172.0
13	1754.5
14	2337.0
15	5753.5
16	9170.0
17	9360.5
18	9551.0
19	9551.0
20	7783.0
21	6015.0
22	5221.0
23	4427.0
24	5111.0
25	5795.0
26	5795.0
27	7248.0
28	8701.0
29	10469.5
30	12238.0
31	15031.5
32	17825.0
33	17825.0
34	22365.0
35	26905.0
36	29751.0
37	32597.0
38	36244.5
39	39892.0
40	39892.0
41	42152.5
42	44413.0
43	47043.0
44	49673.0
45	49739.0
46	49805.0
47	49805.0
48	48991.0
49	48177.0
50	47851.0
51	47525.0
52	56373.0
53	65221.0
54	65221.0
55	52602.5
56	39984.0
57	41925.5
58	43867.0
59	35718.5
60	27570.0
61	27570.0
62	20138.5
63	12707.0
64	9610.0
65	6513.0
66	5120.0
67	3727.0
68	3727.0
69	2747.0
70	1767.0
71	1303.5
72	840.0
73	593.0
74	346.0
75	346.0
76	249.0
77	152.0
78	118.0
79	84.0
80	57.0
81	30.0
82	30.0
83	29.0
84	28.0
85	23.5
86	19.0
87	17.0
88	15.0
89	15.0
90	11.5
91	8.0
92	4.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	620569.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.98606440827335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.162976185446	26.2246587857263
2	11.39370832911129	7.7445461024451365
3	3.6915230936956442	3.7638102488090595
4	1.7028806806180408	2.3149684996435638
5	1.0343387874324717	1.7576552324827668
6	0.6681389094737705	1.3624447206629449
7	0.5131680810394837	1.2208394418134552
8	0.4158915996725493	1.1307614954664877
9	0.33309359703324176	1.0188486398479684
>10	2.48955223856681	17.20443533718217
>50	0.3427816181688649	8.193203928397052
>100	0.23043338906176747	14.175058444470592
>500	0.015298482261362156	3.4457080813356855
>1k	0.003346542994672972	2.27146692021846
>5k	0.0019123102826702695	4.451132781071868
>10k+	9.561551413351348E-4	3.7204613404264877
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	12055	1.9425720588685544	No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG	10843	1.7472674271515336	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	9148	1.4741309991314422	TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC	6180	0.995860250834315	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6086	0.9807128619057671	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5981	0.9637929061877083	No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC	3381	0.5448225741214918	RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	3212	0.5175895025371876	TruSeq Adapter, Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2190	0.3529019335480825	No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG	1586	0.25557190256039214	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1482	0.2388130892777435	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1077	0.17355040293665974	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA	1052	0.16952184205140766	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	973	0.15679158965401108	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	933	0.15034589223760775	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	861	0.13874363688808175	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	858	0.1382602095818515	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	841	0.1355207881798801	No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT	802	0.12923623319888683	No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT	783	0.12617452692609524	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	722	0.11634483836608016	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	706	0.11376655939951882	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	703	0.1132831320932886	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	701	0.11296084722246841	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	691	0.11134942286836758	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	678	0.1092545712080365	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	666	0.1073208619831155	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	642	0.10345344353327349	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	632	0.10184201917917267	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	630	0.1015197343083525	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.6114243541008334E-4	0.0
8	0.0	0.0	0.0	1.6114243541008334E-4	0.0
9	0.0	0.0	0.0	3.222848708201667E-4	0.0
10	0.0	0.0	0.0	3.222848708201667E-4	0.0
11	0.0	0.0	0.0	4.8342730623024996E-4	0.0
12	0.0	0.0	0.0	4.8342730623024996E-4	0.0
13	0.0	0.0	0.0	4.8342730623024996E-4	0.0
14	0.0	0.0	0.0	6.445697416403333E-4	0.0
15	0.0	0.0	0.0	8.057121770504166E-4	0.0
16	0.0	0.0	0.0	9.668546124604999E-4	0.0
17	0.0	0.0	0.0	0.0011279970478705833	0.0
18	0.0	0.0	0.0	0.0012891394832806667	0.0
19	0.0	0.0	0.0	0.0012891394832806667	0.0
20	0.0	0.0	0.0	0.0014502819186907499	0.0
21	0.0	0.0	0.0	0.0022559940957411666	0.0
22	0.0	0.0	0.0	0.0035451335790218333	0.0
23	0.0	0.0	0.0	0.004511988191482333	0.0
24	0.0	0.0	0.0	0.005962270110173083	0.0
25	0.0	0.0	0.0	0.00628455498099325	0.0
26	0.0	0.0	0.0	0.0067679822872235	0.0
27	0.0	0.0	0.0	0.00821826420591425	0.0
28	0.0	0.0	0.0	0.021109659038720915	0.0
29	0.0	0.0	0.0	0.049793012541715746	0.0
30	0.0	0.0	0.0	0.08427749371947359	0.0
31	0.0	0.0	0.0	0.1422887704671036	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGCG	20	0.0018412832	37.0	30
GATTACG	65	1.8189894E-12	34.153847	1
CGTCTTC	350	0.0	32.24286	37
CTTATAC	1465	0.0	31.064846	1
TTATACA	1480	0.0	30.625	2
TATACAC	1605	0.0	28.58567	3
CCGTCTT	1375	0.0	27.178183	37
GGTATCA	955	0.0	27.120419	1
GCCGTCT	1400	0.0	26.560715	36
TTACGGG	110	0.0	25.227272	3
GGCTTGG	45	1.3222542E-4	24.666666	22
CGGGCAC	45	1.3222542E-4	24.666666	17
GTATCAA	2675	0.0	23.514019	1
TCTATAC	110	7.4578566E-11	21.863636	3
CGGCGTA	80	6.9495036E-7	20.8125	12
GGTCACC	80	6.9495036E-7	20.8125	31
TGCCGTC	1790	0.0	20.773743	35
TGGGCCA	45	0.003823855	20.555555	29
CGGGTAT	55	5.1394297E-4	20.181818	16
TTTGGCG	55	5.1394297E-4	20.181818	22
>>END_MODULE