FastQCFastQC Report
Fri 10 Feb 2017
ERR1631220.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631220.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417081
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT104632.5086254228794886TruSeq Adapter, Index 11 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG97332.333599468688336No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT79861.9147359865349896No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC55591.332834629244679No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC37420.8971878364154685RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC29760.7135304653053004No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG13490.3234383728820061No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9740.23352777997559226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9340.22393731673224143No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA8330.19972139704278066No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT7590.18197904004258167No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT7530.18054047055607902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC9100.033.137361
CGTCTTC5600.031.0535737
TCGCCGA250.005492378529.61
ACGGTAC250.005492378529.63
TTATACA10550.028.9336512
TATACAC11550.026.9090923
CCGTCTT17700.025.60734437
GCCGTCT18200.025.00549336
GTATCAA3300.022.4242421
GTACTAT502.698104E-422.21
TGCCGTC21050.021.70783835
ATGCCGT21750.021.00919534
TCACGGT450.00382178320.5555551
TACTAGT759.242574E-619.7333347
TATGCCG23100.019.54112633
CCGTACA1052.2491804E-819.3809517
ACTATCG1002.864017E-718.511
CACGGTA500.00702755218.52
GTACTGG1409.276846E-1118.51
GGTATCA1653.6379788E-1217.9393941
Produced by FastQC (version 0.11.5)