##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631220.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	417081
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.223345105626965	31.0	31.0	34.0	28.0	34.0
2	31.25784439952911	31.0	31.0	34.0	28.0	34.0
3	30.706901057588333	31.0	31.0	34.0	26.0	34.0
4	34.86850995370204	37.0	35.0	37.0	32.0	37.0
5	35.012124743155404	35.0	35.0	37.0	32.0	37.0
6	35.206139814568395	37.0	35.0	37.0	32.0	37.0
7	35.20314039718904	37.0	35.0	37.0	32.0	37.0
8	35.24868550713171	37.0	35.0	37.0	32.0	37.0
9	36.98826846583757	39.0	37.0	39.0	33.0	39.0
10	36.786288514700985	39.0	37.0	39.0	32.0	39.0
11	36.843704220523115	39.0	37.0	39.0	33.0	39.0
12	36.76228358520287	39.0	37.0	39.0	32.0	39.0
13	36.87542947293212	39.0	37.0	39.0	33.0	39.0
14	37.91590842066649	40.0	37.0	41.0	33.0	41.0
15	37.921506853584795	40.0	37.0	41.0	33.0	41.0
16	37.91111798427644	40.0	37.0	41.0	33.0	41.0
17	37.86768517386311	40.0	37.0	41.0	33.0	41.0
18	37.92610068547836	40.0	37.0	41.0	33.0	41.0
19	37.91163347167576	40.0	37.0	41.0	33.0	41.0
20	37.89260839021677	40.0	37.0	41.0	33.0	41.0
21	37.83493853711869	40.0	37.0	41.0	33.0	41.0
22	37.736178344254476	39.0	37.0	41.0	32.0	41.0
23	37.79190373092996	39.0	37.0	41.0	33.0	41.0
24	37.73656196278421	39.0	37.0	41.0	32.0	41.0
25	37.688698358352454	39.0	37.0	41.0	32.0	41.0
26	37.56227926949442	39.0	37.0	41.0	32.0	41.0
27	37.45416357973631	39.0	37.0	41.0	32.0	41.0
28	37.45024108026978	39.0	37.0	40.0	32.0	41.0
29	37.3796960302675	39.0	36.0	40.0	32.0	41.0
30	37.334407465216586	39.0	36.0	40.0	32.0	41.0
31	37.319201785744255	39.0	36.0	40.0	31.0	41.0
32	37.216991423728246	39.0	36.0	40.0	31.0	41.0
33	37.180921691470004	39.0	36.0	40.0	31.0	41.0
34	37.13037995017754	39.0	36.0	40.0	31.0	41.0
35	37.14762839832071	39.0	36.0	40.0	31.0	41.0
36	37.1411884022528	39.0	36.0	40.0	31.0	41.0
37	37.08340106597999	39.0	36.0	40.0	31.0	41.0
38	37.015608478928556	39.0	36.0	40.0	31.0	41.0
39	36.92799240435311	39.0	36.0	40.0	31.0	41.0
40	36.79306897221403	39.0	36.0	40.0	30.0	41.0
41	36.78530549221854	39.0	35.0	40.0	30.0	41.0
42	36.7535946255044	39.0	35.0	40.0	30.0	41.0
43	36.04616369482187	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	14.0
11	11.0
12	13.0
13	12.0
14	7.0
15	9.0
16	11.0
17	10.0
18	19.0
19	28.0
20	46.0
21	108.0
22	210.0
23	387.0
24	691.0
25	1168.0
26	1883.0
27	2887.0
28	4245.0
29	6120.0
30	8149.0
31	11056.0
32	14065.0
33	18203.0
34	23843.0
35	31665.0
36	43884.0
37	64239.0
38	105723.0
39	78368.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.41993281880498	24.491405746126052	16.539473147901727	24.54918828716724
2	13.280154214648954	21.687633816932443	40.81868989476864	24.213522073649962
3	14.4036769836075	28.92435761878388	34.892742656702175	21.77922274090644
4	11.408095789546875	19.577252380233094	36.1776249697301	32.83702686048993
5	12.410538960058117	38.36520963553842	35.080236213109686	14.144015191293777
6	27.69893617786473	39.37076970660375	17.76441506565871	15.165879049872807
7	23.160489209530045	34.773581150903546	22.22613832804659	19.839791311519825
8	25.034465727280793	34.711482901402846	22.856471524715822	17.397579846600543
9	22.52128483436071	15.07524917222314	19.220966670742616	43.182499322673536
10	13.828968473749704	28.139857725477786	33.8258036208794	24.205370179893116
11	30.36820186007035	22.506419616333517	24.92177778417142	22.20360073942472
12	19.098448502808807	27.197354950237486	28.944737353176002	24.759459193777708
13	29.256906931747075	20.10400857387414	26.070955042305933	24.56812945207286
14	21.721919723027423	23.177032758624826	25.555947166137994	29.545100352209765
15	23.7771559960775	29.54102440533134	21.91588684212419	24.765932756466967
16	25.49912367142114	27.46804577528106	22.87852959017553	24.15430096312227
17	19.60410567731448	32.493688276377966	24.429547258206437	23.47265878810111
18	22.923365005838196	25.397224999460537	24.785832967696923	26.893577027004344
19	22.936791654378887	28.355163625291013	24.774564173385986	23.933480546944118
20	23.395695320573225	26.327020410903394	25.661202500233767	24.616081768289614
21	24.015479007674767	25.907197882425713	27.441672001361844	22.63565110853767
22	20.676079706340015	33.27507126912998	24.927771823698514	21.121077200831493
23	22.515290794833618	24.74363492942618	26.703206331623836	26.03786794411637
24	22.264020657857824	29.055507203636704	27.502091919794957	21.17838021871051
25	22.504501523684848	29.746500080320132	25.203737403525935	22.54526099246909
26	24.53576163862655	26.32510231825473	26.786643361840984	22.352492681277738
27	22.4608649159276	31.593143777827326	25.568414768354348	20.37757653789072
28	20.678717083731936	24.75274586950736	30.369400667975764	24.199136378784935
29	20.47587878613507	27.97178485713806	27.120391482709593	24.43194487401728
30	22.333791277953203	26.293214027970585	28.382016922372394	22.990977771703818
31	22.655071796605455	24.887012354914273	29.500744459709267	22.957171388771005
32	22.174110064951414	30.638892685113923	27.13693503180437	20.05006221813029
33	22.198565746221956	25.696687214234164	29.582742920439912	22.522004119103965
34	20.737938194259627	29.68919706244111	28.37026860489929	21.202596138399976
35	21.105252936479964	26.79791215615192	32.2656750127673	19.83115989460081
36	25.26056089824279	25.45740515631256	24.84936978668412	24.432664158760527
37	21.39560421117241	26.579249594203525	28.557042876563543	23.46810331806052
38	21.654546718742882	24.593304418086653	28.574305710401575	25.177843152768887
39	25.178322675931053	25.615408038246766	26.54832035024372	22.65794893557846
40	20.436318125256246	24.299596481259037	33.561346596944	21.702738796540718
41	22.358966243967	26.294892359038176	26.219367460996786	25.12677393599804
42	21.89790472354291	24.562614935707934	31.86647198026283	21.67300836048633
43	22.70494220547088	24.305350759205048	30.11525339202697	22.874453643297105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	6.5
2	9.0
3	29.5
4	50.0
5	50.0
6	74.5
7	99.0
8	101.5
9	104.0
10	168.0
11	232.0
12	232.0
13	373.5
14	515.0
15	1174.0
16	1833.0
17	2166.5
18	2500.0
19	2500.0
20	2790.5
21	3081.0
22	3867.5
23	4654.0
24	6057.5
25	7461.0
26	7461.0
27	9463.0
28	11465.0
29	13171.5
30	14878.0
31	16775.0
32	18672.0
33	18672.0
34	20659.5
35	22647.0
36	24004.0
37	25361.0
38	26525.5
39	27690.0
40	27690.0
41	28041.5
42	28393.0
43	28553.0
44	28713.0
45	28410.0
46	28107.0
47	28107.0
48	28187.5
49	28268.0
50	35662.5
51	43057.0
52	32980.0
53	22903.0
54	22903.0
55	29689.5
56	36476.0
57	30191.0
58	23906.0
59	17895.5
60	11885.0
61	11885.0
62	10179.5
63	8474.0
64	7192.5
65	5911.0
66	4843.5
67	3776.0
68	3776.0
69	3149.5
70	2523.0
71	2019.5
72	1516.0
73	1198.0
74	880.0
75	880.0
76	686.0
77	492.0
78	395.5
79	299.0
80	227.0
81	155.0
82	155.0
83	109.0
84	63.0
85	40.5
86	18.0
87	13.0
88	8.0
89	8.0
90	5.0
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	417081.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.22934977694301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.11820149138248	60.31136442985117
2	8.647042912857065	11.972583167008361
3	2.0526553503257414	4.263119856576428
4	0.7681684896297627	2.1271922022481946
5	0.39615923854457435	1.3712923246284874
6	0.24309610737179096	1.0097631267993008
7	0.15711063318251872	0.7613666884788703
8	0.11016856552612461	0.6101518525785719
9	0.07603118745730088	0.4737230703394025
>10	0.4120179760647625	4.967828303715546
>50	0.010365025538165366	0.48411649728263195
>100	0.00483701191060122	0.6302603601137515
>500	0.0017275042537861498	1.0172665319027647
>1k	0.0010365025522716899	1.9295295351186466
>5k	0.0010365025522716899	5.567818088321787
>10k+	3.4550085075723E-4	2.5026239650361224
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	10463	2.5086254228794886	TruSeq Adapter, Index 11 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG	9733	2.333599468688336	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	7986	1.9147359865349896	No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	5559	1.332834629244679	No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC	3742	0.8971878364154685	RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	2976	0.7135304653053004	No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG	1349	0.3234383728820061	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	974	0.23352777997559226	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	934	0.22393731673224143	No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA	833	0.19972139704278066	No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT	759	0.18197904004258167	No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	753	0.18054047055607902	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.397615810837703E-4	0.0
11	0.0	0.0	0.0	2.397615810837703E-4	0.0
12	0.0	0.0	0.0	2.397615810837703E-4	0.0
13	0.0	0.0	0.0	2.397615810837703E-4	0.0
14	0.0	0.0	0.0	4.795231621675406E-4	0.0
15	0.0	0.0	0.0	4.795231621675406E-4	0.0
16	0.0	0.0	0.0	4.795231621675406E-4	0.0
17	0.0	0.0	0.0	4.795231621675406E-4	0.0
18	0.0	0.0	0.0	7.192847432513109E-4	0.0
19	0.0	0.0	0.0	7.192847432513109E-4	0.0
20	0.0	0.0	0.0	0.0011988079054188515	0.0
21	0.0	0.0	0.0	0.0014385694865026218	0.0
22	0.0	0.0	0.0	0.0014385694865026218	0.0
23	0.0	0.0	0.0	0.0014385694865026218	0.0
24	0.0	0.0	0.0	0.0014385694865026218	0.0
25	0.0	0.0	0.0	0.0014385694865026218	0.0
26	0.0	0.0	0.0	0.0014385694865026218	0.0
27	0.0	0.0	0.0	0.0021578542297539327	0.0
28	0.0	0.0	0.0	0.004555470040591636	0.0
29	0.0	0.0	0.0	0.009350701662267041	0.0
30	0.0	0.0	0.0	0.01630378751369638	0.0
31	0.0	0.0	0.0	0.03572447558148178	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	910	0.0	33.13736	1
CGTCTTC	560	0.0	31.05357	37
TCGCCGA	25	0.0054923785	29.6	1
ACGGTAC	25	0.0054923785	29.6	3
TTATACA	1055	0.0	28.933651	2
TATACAC	1155	0.0	26.909092	3
CCGTCTT	1770	0.0	25.607344	37
GCCGTCT	1820	0.0	25.005493	36
GTATCAA	330	0.0	22.424242	1
GTACTAT	50	2.698104E-4	22.2	1
TGCCGTC	2105	0.0	21.707838	35
ATGCCGT	2175	0.0	21.009195	34
TCACGGT	45	0.003821783	20.555555	1
TACTAGT	75	9.242574E-6	19.733334	7
TATGCCG	2310	0.0	19.541126	33
CCGTACA	105	2.2491804E-8	19.38095	17
ACTATCG	100	2.864017E-7	18.5	11
CACGGTA	50	0.007027552	18.5	2
GTACTGG	140	9.276846E-11	18.5	1
GGTATCA	165	3.6379788E-12	17.939394	1
>>END_MODULE