FastQCFastQC Report
Fri 10 Feb 2017
ERR1631219.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631219.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374350
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78812.1052490984372914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77552.0715907573126753No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA31830.8502738079337518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25240.6742353412581809No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA22520.6015760651796447No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA17400.46480566314945904No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16640.4445038065981034No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG13760.36757045545612393No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT13590.36302925070121544No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT13510.3608922131694938No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13390.3576866568719113No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC13000.3472685989047683No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT12290.3283023908107386No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG12100.3232269266728997No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG11500.3071991451849873No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10310.27541071190062777No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10130.27060237745425403No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG10090.26953385868839325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9300.24843061306264194No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG9280.2478963536797115No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC8870.23694403632963804No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8720.23293709095765994No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8640.2308000534259383No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG8600.22973153466007748No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8370.2235875517563777No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT7890.21076532656604782No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7760.20729264057700014No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7680.20515560304527847No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.2024843061306264No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7540.2014157873647656No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7360.19660745291839188No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT7060.18859356217443568No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7000.18699078402564445No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6930.185120876185388No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6900.18431948711099239No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6800.18164819019634032No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6790.18138106050487512No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6730.1797782823560839No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6710.17924402297315348No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6450.1722986509950581No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6400.17096300253773206No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6200.16562040870842792No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6060.16188059302791505No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5930.15840790703886737No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA5920.15814077734740214No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA5680.1517296647522372No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5590.14932549752905036No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5390.14398290369974623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC5340.1426472552424202No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC5330.14238012555095497No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC5300.14157873647655939No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5290.14131160678509416No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5200.1389074395619073No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG5000.13356484573260316No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4690.12528382529718177No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4620.12341391745692533No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4590.12261252838252973No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4560.12181113930813409No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4550.12154400961666889No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4540.12127687992520368No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4360.11646854547882997No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4320.11540002671296914No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4310.11513289702150395No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC4300.11486576733003874No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG4300.11486576733003874No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA4270.11406437825564311No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4190.11192734072392146No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4150.11085882195806063No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4020.10738613596901296No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4010.10711900627754777No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3970.10605048751168693No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3930.10498196874582609No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3930.10498196874582609No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT3900.10418057967143048No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3860.10311206090556965No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3770.1007078936823828No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT3760.10044076399091759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG452.6102498E-936.9999963
GATTACG501.7826096E-1036.9999961
CTCAGAG1050.031.7142852
ATTACGG601.2641976E-930.8333342
GGGATCA551.848639E-830.2727281
AGGTAAT250.0054916729.5999981
TGCCACG250.0054916729.59999823
GACGGAA250.0054916729.59999831
TTGCCAC250.0054916729.59999822
GGACCCG250.0054916729.59999832
ACTCAGA1000.029.5999981
AGCACGG250.0054916729.5999982
GGTATCA7700.029.0714281
TAGTTCC652.9758667E-928.4615384
TATACAC2050.027.975613
TCAGAGT1000.027.7499983
TTACTTT358.858179E-426.4285723
GTATCAA25800.025.5988371
GCCTAGT701.9157233E-723.7857151
TTATACA2450.023.4081652
Produced by FastQC (version 0.11.5)