FastQCFastQC Report
Fri 10 Feb 2017
ERR1631218.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631218.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458067
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT149783.269827339668651No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG140923.076405853292204No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT117062.555521353863081TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC81181.7722298266410808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68011.4847173011808317No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64381.405471252022084No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC42320.9238823141592825No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC40110.8756360969028548RNA PCR Primer, Index 1 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA29380.6413908882325075No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22790.49752547116469864No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG19300.42133574346110936No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA19200.41915265670742485No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA19160.4182794220059511No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT16390.3578079189288903No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT13430.293188551019829No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA13130.2866392907587755No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT12490.2726675355351946No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11920.260223941039193No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11820.25804085428550844No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11820.25804085428550844No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT11240.24537895111413832No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11010.24035785158066394No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT10090.2202734534467665No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8710.19014685624592034No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8620.18818207816760432No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8170.17835818777602402No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.1781398791006556No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8110.17704833572381334No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7750.1691892234105491No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7610.1661329019553908No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG7610.1661329019553908No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7270.15871040699286348No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7270.15871040699286348No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG7190.1569639375899159No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6960.15194283805644151No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6740.14714004719833562No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6560.1432104910417035No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6530.14255556501559816No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6500.1419006389894928No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6340.13840770018359758No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6020.13142182257180718No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5970.13033027919496493No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5770.12596410568759592No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.125527488336859No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5520.12050638880338464No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5470.1194148454265424No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5370.11723175867285791No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5190.11330220251622579No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5120.11177404178864664No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4940.10784448563201453No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4820.10522478152759313No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4800.10478816417685621No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4730.10326000344927706No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4690.10238676874780328No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4650.10151353404632946No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4630.10107691669559257No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4620.10085860802022412No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4600.10042199066948722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACA308.301067E-637.02
GATACAC308.301067E-637.03
GGTCGCT355.621441E-737.028
GGTCCGC355.621441E-737.09
TCTCGCG355.621441E-737.020
TCACTTA200.001840780337.035
CTTTATG355.621441E-737.01
GTAAGGC251.2324666E-437.016
AGGTCCG355.621441E-737.08
CTTATAC16900.034.5917171
GATTACG750.034.5333331
CGTCTTC5200.033.79807737
TTATACA18100.032.400552
ATTACTT401.5972855E-632.3752
CGCCGAG401.5972855E-632.3752
CTCTCGC401.5972855E-632.37519
GACCGTT705.456968E-1231.7142857
CGAGGTT352.3851953E-531.71428522
TAGCGAG303.596129E-430.83333228
TTATCAA303.596129E-430.8333322
Produced by FastQC (version 0.11.5)