##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631218.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458067
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29651339214569	31.0	31.0	34.0	28.0	34.0
2	31.282153047479955	31.0	31.0	34.0	28.0	34.0
3	30.71336289232798	31.0	31.0	34.0	26.0	34.0
4	34.886206602964194	37.0	35.0	37.0	32.0	37.0
5	35.12663431332098	35.0	35.0	37.0	32.0	37.0
6	35.34774607208116	37.0	35.0	37.0	33.0	37.0
7	35.35429969851572	37.0	35.0	37.0	33.0	37.0
8	35.37473120744345	37.0	35.0	37.0	33.0	37.0
9	37.160653354203646	39.0	37.0	39.0	34.0	39.0
10	36.98601078008239	39.0	37.0	39.0	33.0	39.0
11	37.03760366933221	39.0	37.0	39.0	33.0	39.0
12	36.92549561527026	39.0	37.0	39.0	33.0	39.0
13	37.0253019754752	39.0	37.0	39.0	33.0	39.0
14	37.95189350029581	40.0	37.0	41.0	33.0	41.0
15	37.96047303123779	40.0	37.0	41.0	33.0	41.0
16	37.982869318243836	40.0	37.0	41.0	33.0	41.0
17	38.00147576664549	40.0	37.0	41.0	33.0	41.0
18	38.09681116517889	40.0	37.0	41.0	33.0	41.0
19	38.067599281327844	40.0	37.0	41.0	33.0	41.0
20	38.10207022116852	40.0	37.0	41.0	33.0	41.0
21	38.04061414596555	40.0	37.0	41.0	33.0	41.0
22	37.912111110383414	40.0	37.0	41.0	33.0	41.0
23	37.94139940227085	40.0	37.0	41.0	33.0	41.0
24	37.79389041341114	40.0	37.0	41.0	33.0	41.0
25	37.774607208115846	39.0	37.0	41.0	33.0	41.0
26	37.59715718442935	39.0	37.0	41.0	32.0	41.0
27	37.451853113190865	39.0	36.0	41.0	32.0	41.0
28	37.49092381682156	39.0	36.0	41.0	32.0	41.0
29	37.36052149576372	39.0	36.0	41.0	32.0	41.0
30	37.18004789692338	39.0	36.0	40.0	31.0	41.0
31	37.079577878345304	39.0	35.0	40.0	31.0	41.0
32	36.86570960143385	39.0	35.0	40.0	31.0	41.0
33	36.76927611026335	39.0	35.0	40.0	31.0	41.0
34	36.62365330835882	39.0	35.0	40.0	30.0	41.0
35	36.54554246431199	39.0	35.0	40.0	30.0	41.0
36	36.46027546188658	39.0	35.0	40.0	30.0	41.0
37	36.32489133685684	38.0	35.0	40.0	30.0	41.0
38	36.22366378717524	38.0	35.0	40.0	30.0	41.0
39	36.07935956966994	38.0	35.0	40.0	30.0	41.0
40	35.81921640284063	38.0	35.0	40.0	28.0	41.0
41	35.71042009138401	38.0	35.0	40.0	28.0	41.0
42	35.50232607893605	38.0	34.0	40.0	27.0	41.0
43	34.68148545955068	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	11.0
11	15.0
12	14.0
13	8.0
14	7.0
15	11.0
16	4.0
17	10.0
18	29.0
19	44.0
20	84.0
21	172.0
22	295.0
23	642.0
24	1056.0
25	1704.0
26	2732.0
27	3978.0
28	5693.0
29	7811.0
30	10199.0
31	12862.0
32	16260.0
33	20498.0
34	26766.0
35	35289.0
36	47715.0
37	70848.0
38	103274.0
39	90027.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.72614049909729	22.263773640100684	16.466368457016113	23.54371740378591
2	13.75322823953701	22.5508495482102	41.06211536740258	22.633806844850206
3	14.863109545110213	30.652066182458025	34.03257602053848	20.45224825189328
4	10.432534978507512	19.501077353312944	34.176441437606286	35.88994623057326
5	13.17427363245988	38.06299078519081	31.886165124315873	16.87657045803343
6	26.18940024057616	39.293378479567394	16.44606575020685	18.071155529649594
7	23.517738671417064	32.90894126841707	19.21378313652806	24.35953692363781
8	26.97400161985037	30.562777934232326	22.868270362195922	19.594950083721375
9	23.513372497909693	12.539213695813059	17.309258252613702	46.638155553663545
10	16.36812955310031	27.22331012712114	29.38609417399638	27.022466145782165
11	31.96322808672094	21.970585089080856	22.780728583373175	23.285458240825033
12	21.271997327901815	27.509731109204548	24.492486906937195	26.725784655956442
13	33.11851759677078	19.260064575706174	24.553176718689624	23.068241108833423
14	23.024361065084364	22.986375355570253	26.14159064067047	27.84767293867491
15	26.71923539569539	26.223456393933635	22.059436719955812	24.997871490415157
16	25.404362243951212	24.075517336983452	21.500348202337214	29.019772216728118
17	20.450283473814963	30.237934625284073	22.417244638884704	26.894537262016254
18	25.26202498761098	21.19951884767949	25.595818952249346	27.942637212460188
19	25.264644691715404	27.009804242610798	26.457483293928618	21.26806777174518
20	22.581631071437148	21.257588955327495	28.759766584364293	27.40101338887106
21	26.03156306828477	22.474659820506606	26.737355015750968	24.75642209545765
22	19.674196132880127	30.143406968849533	29.362735145731953	20.819661752538384
23	23.28305684539598	22.404146118362597	26.76682668692571	27.545970349315713
24	23.78996958960152	29.19070790954162	26.662256831424223	20.357065669432636
25	23.890391580271007	25.802775576498632	27.766898728788586	22.539934114441774
26	26.352476821076394	27.166331562849976	25.730733713627046	20.750457902446584
27	21.215018763630646	27.632638893436983	31.900136879539453	19.25220546339291
28	19.49190838894747	23.052304575531526	29.023265155534016	28.43252187998699
29	21.285750774450026	27.715377881401633	28.871322317477578	22.12754902667077
30	21.58221395560038	21.64181222397597	33.57740243239526	23.198571388028387
31	24.695077357679114	23.704174280181718	25.24106735477561	26.35968100736355
32	23.765300709284887	23.016283644095733	31.836390746331865	21.382024900287515
33	22.533821471531457	22.52072295100935	30.831079296260157	24.11437628119904
34	19.84404028231678	30.218941770527017	27.297316768071045	22.639701179085154
35	23.95042646599733	22.90953070184056	33.82125322278182	19.31878960938029
36	26.845417810058354	23.00558651900268	25.402834083223635	24.746161587715335
37	22.36288577871796	25.223602660746135	29.107532304226236	23.305979256309666
38	21.63024186418144	21.73874127583956	29.59916344115599	27.031853418823008
39	25.648867960363873	22.40087148823207	27.50427339232034	24.445987159083714
40	20.024799865521857	22.668736232909158	35.71289789484944	21.59356600671954
41	23.815948321970367	23.093346606500795	25.91062006213065	27.180085009398187
42	22.823298775070022	22.26268209672384	32.483675968799325	22.430343159406814
43	23.98011644584744	21.61888981306228	30.735023479098032	23.665970261992243
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	21.0
1	20.0
2	19.0
3	117.0
4	215.0
5	215.0
6	327.0
7	439.0
8	489.5
9	540.0
10	813.0
11	1086.0
12	1086.0
13	1732.0
14	2378.0
15	6144.0
16	9910.0
17	9998.5
18	10087.0
19	10087.0
20	7988.5
21	5890.0
22	4629.5
23	3369.0
24	3391.0
25	3413.0
26	3413.0
27	3751.0
28	4089.0
29	4559.0
30	5029.0
31	6220.0
32	7411.0
33	7411.0
34	9379.5
35	11348.0
36	12204.0
37	13060.0
38	14864.0
39	16668.0
40	16668.0
41	18511.0
42	20354.0
43	23255.0
44	26156.0
45	29857.5
46	33559.0
47	33559.0
48	39123.0
49	44687.0
50	56537.0
51	68387.0
52	54216.5
53	40046.0
54	40046.0
55	48312.5
56	56579.0
57	46198.0
58	35817.0
59	25515.0
60	15213.0
61	15213.0
62	12648.0
63	10083.0
64	7888.0
65	5693.0
66	4531.5
67	3370.0
68	3370.0
69	2686.5
70	2003.0
71	1403.0
72	803.0
73	465.0
74	127.0
75	127.0
76	107.0
77	87.0
78	67.0
79	47.0
80	38.5
81	30.0
82	30.0
83	24.0
84	18.0
85	21.5
86	25.0
87	17.0
88	9.0
89	9.0
90	5.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	458067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.695444116253736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.63893079040918	14.619040446048285
2	10.886190782603192	4.505891059604818
3	4.510596103334423	2.8004636876264826
4	2.615006487410205	2.1647488249535547
5	1.7531830504541188	1.8141450923118234
6	1.3428411692106457	1.6674416624642245
7	0.9852424603635059	1.427302119558929
8	0.7932573128408527	1.3133449910165982
9	0.6329180687559995	1.1788668469896326
>10	4.792244643930843	19.97786350031764
>50	0.5928332577347862	8.49962996679525
>100	0.40506756400383975	15.728921751621488
>500	0.027426449646093316	3.8483453293950447
>1k	0.017932678614753318	6.889821794628297
>5k	0.003164590343779998	4.662418379843997
>10k+	0.003164590343779998	8.901754546823938
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	14978	3.269827339668651	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	14092	3.076405853292204	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	11706	2.555521353863081	TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	8118	1.7722298266410808	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6801	1.4847173011808317	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6438	1.405471252022084	No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	4232	0.9238823141592825	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	4011	0.8756360969028548	RNA PCR Primer, Index 1 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	2938	0.6413908882325075	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2279	0.49752547116469864	No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG	1930	0.42133574346110936	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1920	0.41915265670742485	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1916	0.4182794220059511	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1639	0.3578079189288903	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1343	0.293188551019829	No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA	1313	0.2866392907587755	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1249	0.2726675355351946	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	1192	0.260223941039193	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	1182	0.25804085428550844	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	1182	0.25804085428550844	No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT	1124	0.24537895111413832	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	1101	0.24035785158066394	No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT	1009	0.2202734534467665	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	871	0.19014685624592034	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	862	0.18818207816760432	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	817	0.17835818777602402	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	816	0.1781398791006556	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	811	0.17704833572381334	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	775	0.1691892234105491	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	761	0.1661329019553908	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	761	0.1661329019553908	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	727	0.15871040699286348	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	727	0.15871040699286348	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	719	0.1569639375899159	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	696	0.15194283805644151	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	674	0.14714004719833562	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	656	0.1432104910417035	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	653	0.14255556501559816	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	650	0.1419006389894928	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	634	0.13840770018359758	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	602	0.13142182257180718	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	597	0.13033027919496493	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	577	0.12596410568759592	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	575	0.125527488336859	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	552	0.12050638880338464	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	547	0.1194148454265424	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	537	0.11723175867285791	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	519	0.11330220251622579	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	512	0.11177404178864664	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	494	0.10784448563201453	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	482	0.10522478152759313	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	480	0.10478816417685621	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	473	0.10326000344927706	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	469	0.10238676874780328	No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA	465	0.10151353404632946	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	463	0.10107691669559257	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	462	0.10085860802022412	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	460	0.10042199066948722	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	2.1830867536845047E-4	0.0
21	0.0	0.0	0.0	4.3661735073690093E-4	0.0
22	0.0	0.0	0.0	0.0010915433768422524	0.0
23	0.0	0.0	0.0	0.0024013954290529552	0.0
24	0.0	0.0	0.0	0.003929556156632108	0.0
25	0.0	0.0	0.0	0.00458448218273746	0.0
26	0.0	0.0	0.0	0.0048027908581059105	0.0
27	0.0	0.0	0.0	0.007640803637895766	0.0
28	0.0	0.0	0.0	0.0237956456151611	0.0
29	0.0	0.0	0.0	0.05741518162190247	0.0
30	0.0	0.0	0.0	0.09780228656506582	0.0
31	0.0	0.0	0.0	0.16416812387707475	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATACA	30	8.301067E-6	37.0	2
GATACAC	30	8.301067E-6	37.0	3
GGTCGCT	35	5.621441E-7	37.0	28
GGTCCGC	35	5.621441E-7	37.0	9
TCTCGCG	35	5.621441E-7	37.0	20
TCACTTA	20	0.0018407803	37.0	35
CTTTATG	35	5.621441E-7	37.0	1
GTAAGGC	25	1.2324666E-4	37.0	16
AGGTCCG	35	5.621441E-7	37.0	8
CTTATAC	1690	0.0	34.591717	1
GATTACG	75	0.0	34.533333	1
CGTCTTC	520	0.0	33.798077	37
TTATACA	1810	0.0	32.40055	2
ATTACTT	40	1.5972855E-6	32.375	2
CGCCGAG	40	1.5972855E-6	32.375	2
CTCTCGC	40	1.5972855E-6	32.375	19
GACCGTT	70	5.456968E-12	31.714285	7
CGAGGTT	35	2.3851953E-5	31.714285	22
TAGCGAG	30	3.596129E-4	30.833332	28
TTATCAA	30	3.596129E-4	30.833332	2
>>END_MODULE