FastQCFastQC Report
Fri 10 Feb 2017
ERR1631217.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631217.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355553
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90092.5337994616836306No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83232.3408605749353826No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA33410.9396630038278401No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT27260.7666930106060137No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA23990.6747235995758719No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG22290.6269107559210582No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT21630.6083481225021304No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT20060.5641915551268025RNA PCR Primer, Index 29 (95% over 23bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA18310.5149724513644942No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT17780.5000660942250522No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT14320.4027528947864313No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14230.40022162659294114No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG14080.3960028462704576No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC13870.39009655381898056No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC13200.371252668378554No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC12840.3611275956045934No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG11990.3372211737771865No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11630.32709610100322595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11430.32147106057324787No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG10960.30825221556279936No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9840.27675198915492205No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9080.25537683552100526No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG8940.25143930722002067No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8930.25115805519852175No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG8860.2491892910480294No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8740.24581426679004253No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8490.23878296625256995No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8390.23597044603758088No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8290.23315792582259184No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC8220.23118916167209955RNA PCR Primer, Index 29 (95% over 21bp)
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8030.2258453732636203No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7920.22275160102713237No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7680.21600155251115866No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7360.20700148782319375No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7230.203345211543708No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6930.19490765089874082No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6910.19434514685574303No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6900.19406389483424413No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC6840.1923763827052507RNA PCR Primer, Index 29 (95% over 24bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6830.19209513068375178No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6780.19068887057625727No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6650.18703259429677152No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6430.18084504982379562No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC6370.17915753769480217No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC6160.1732512452433252No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6070.17071997704983505No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6030.16959496896383944No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5960.16762620481334708No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5790.16284492044786572No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5740.1614386603403712No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC5620.15806363608238436No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5610.15778238406088543No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5610.15778238406088543No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA5550.156094871931892No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA5320.1496260754374172No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5250.14765731128692489No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC5080.1428760269214435No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4940.13893849862045882No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4930.13865724659895992No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG4850.1364072304269687No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4670.1313446940399884No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4630.1302196859539928No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4590.12909467786799717No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4460.1254384015885114No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4420.1243133935025158No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA4380.12318838541652019No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4370.12290713339502127No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA4370.12290713339502127No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4360.12262588137352237No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4260.11981336115853333No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4260.11981336115853333No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC4160.11700084094354428No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA4160.11700084094354428No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT4160.11700084094354428No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4160.11700084094354428No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT4130.11615708487904756No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4080.11475082477155303No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3930.11053204444906949No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3910.10996954040607167No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3890.10940703636307385No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3890.10940703636307385No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA3880.10912578434157495No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT3830.10771952423408043No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA3820.10743827221258154No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3800.10687576816958372No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3760.10575076008358811No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3760.10575076008358811No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC3720.10462575199759248No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3660.10293823986859905No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3650.10265698784710015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCT251.2319113E-437.035
GATTACG950.037.01
ATTACGG1000.035.152
TTACGGG1000.035.153
TTACTTT401.5959558E-632.3753
TCAGAGT1000.031.453
CTCAGAG1000.031.452
GGTGTTC303.5945233E-430.8333348
CCTAGTT303.5945233E-430.8333342
ACTCAGA1100.030.2727281
TAGCAAC751.4551915E-1129.61
GTGAAAG250.005491302329.636
TTCTCTG250.005491302329.67
ACTATAT652.9758667E-928.4615383
CTATCTA652.9758667E-928.46153816
CGTTATT604.305184E-827.75000210
AGGTGGC604.305184E-827.75000210
GTTGACA405.9287726E-527.752
TTGACAG405.9287726E-527.753
CTTATAC3000.027.1333331
Produced by FastQC (version 0.11.5)