FastQCFastQC Report
Fri 10 Feb 2017
ERR1631216.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631216.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688960
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT72051.0457791453785417No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG70941.0296679052484905No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT65990.9578204830469114TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC38700.5617162099396191No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC20340.29522758941012545RNA PCR Primer, Index 8 (95% over 23bp)
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT18920.27461681374825825No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC18880.2740362285183465No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC17600.25545750116117044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16880.24500696702275895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15370.22308987459359036No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC14860.21568741291221552No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT13770.1998664653971203No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG13700.19885044124477472No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA13380.1942057594054807No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC12250.17780422666047374No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT12160.17649790989317232No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA11550.16764398513701811No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT11450.16619252206223875No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG11040.1602415234556433No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA11020.1599512308406874No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC10380.1506618671620994No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA10280.14921040408732003No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG9580.13905016256386438No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG9360.13585694379934973No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC9180.13324431026474687No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA9000.130631676730144No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG8890.12903506734788667No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT8680.12598699489084997No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC8630.1252612633534603No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG7670.11132721783557827No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA7320.10624709707385044No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG7160.10392475615420343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC9350.032.8449171
TTATACA10550.029.9857832
CGTCTTC2800.028.41071537
TATACAC11400.027.912283
CCGTCTT9400.026.56914937
GCCGTCT9850.025.35533136
GGTATCA3250.025.0461561
GTATCAA9750.024.856411
ACGGTAC451.3224603E-424.6666683
CACGGTA502.7005796E-422.22
CGGTACT502.7005796E-422.24
TGCCGTC11650.021.43776935
GACGTAG450.00382427520.55555725
ATGCCGT12700.019.66535434
AGACGTA500.00703209118.524
TCTCGTG500.00703209118.524
ACCGTGC902.149669E-618.59
TATGCCG13800.018.09782633
AACGGCC752.0657519E-417.26666637
TCACGGT650.001579065817.0769231
Produced by FastQC (version 0.11.5)