##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631216.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	688960
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.147684916395725	31.0	31.0	34.0	28.0	34.0
2	31.14792585926614	31.0	31.0	34.0	28.0	34.0
3	30.563594403158383	31.0	30.0	34.0	26.0	34.0
4	34.78312529029262	37.0	35.0	37.0	32.0	37.0
5	34.954066999535534	35.0	35.0	37.0	32.0	37.0
6	35.1674320715281	37.0	35.0	37.0	32.0	37.0
7	35.17431055503948	37.0	35.0	37.0	32.0	37.0
8	35.21786751045053	37.0	35.0	37.0	32.0	37.0
9	36.93648833023688	39.0	37.0	39.0	33.0	39.0
10	36.72503048072457	38.0	37.0	39.0	32.0	39.0
11	36.80482466326057	39.0	37.0	39.0	33.0	39.0
12	36.72801323734324	38.0	37.0	39.0	32.0	39.0
13	36.815871748722714	39.0	37.0	39.0	33.0	39.0
14	37.88695279842081	39.0	37.0	41.0	33.0	41.0
15	37.89230434277752	39.0	37.0	41.0	33.0	41.0
16	37.883958430097536	39.0	37.0	41.0	33.0	41.0
17	37.842442231769624	39.0	37.0	41.0	33.0	41.0
18	37.88738098002787	39.0	37.0	41.0	33.0	41.0
19	37.93203669298653	40.0	37.0	41.0	33.0	41.0
20	37.93024123316303	40.0	37.0	41.0	33.0	41.0
21	37.88862343241988	40.0	37.0	41.0	33.0	41.0
22	37.81281206456108	39.0	37.0	41.0	33.0	41.0
23	37.802412331630286	39.0	37.0	41.0	33.0	41.0
24	37.749612459359035	39.0	37.0	41.0	32.0	41.0
25	37.71624477473293	39.0	37.0	41.0	32.0	41.0
26	37.60679720157919	39.0	37.0	41.0	32.0	41.0
27	37.54261931026475	39.0	37.0	40.0	32.0	41.0
28	37.54085433116582	39.0	37.0	40.0	32.0	41.0
29	37.48859440315838	39.0	37.0	40.0	32.0	41.0
30	37.41697340919647	39.0	36.0	40.0	32.0	41.0
31	37.37927455875523	39.0	36.0	40.0	32.0	41.0
32	37.29942667208546	39.0	36.0	40.0	31.0	41.0
33	37.24503599628425	39.0	36.0	40.0	31.0	41.0
34	37.18269565722248	39.0	36.0	40.0	31.0	41.0
35	37.18121951927543	39.0	36.0	40.0	31.0	41.0
36	37.140172143520665	39.0	36.0	40.0	31.0	41.0
37	37.08987023920111	39.0	36.0	40.0	31.0	41.0
38	37.042135973060844	39.0	36.0	40.0	31.0	41.0
39	36.95948676265676	39.0	36.0	40.0	31.0	41.0
40	36.8867974918718	39.0	36.0	40.0	31.0	41.0
41	36.85252264282396	39.0	36.0	40.0	30.0	41.0
42	36.81638266372504	39.0	35.0	40.0	30.0	41.0
43	36.14361936832327	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	3.0
10	13.0
11	8.0
12	7.0
13	4.0
14	1.0
15	5.0
16	5.0
17	10.0
18	16.0
19	37.0
20	80.0
21	141.0
22	276.0
23	582.0
24	1006.0
25	1792.0
26	2989.0
27	4533.0
28	6802.0
29	9397.0
30	13500.0
31	17643.0
32	23156.0
33	30428.0
34	39514.0
35	53012.0
36	74528.0
37	110574.0
38	176962.0
39	121932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.354040873200184	23.4401126335346	15.576956572224804	22.62888992104041
2	14.362662563864376	23.330672317696237	40.74880980027868	21.55785531816071
3	15.469548304691127	28.869890849976777	34.81218067812355	20.848380167208546
4	12.138005109150022	19.58052717138876	38.41558290757084	29.865884811890385
5	11.779638875986995	39.722770552717144	35.99222015791918	12.505370413376685
6	27.297230608453322	40.669995355318164	18.142998142127265	13.889775894101252
7	23.46856130980028	34.368468416163495	23.78512540640966	18.377844867626568
8	23.748403390617742	37.05193334881561	21.953379006038087	17.246284254528565
9	23.48670459823502	16.237952856479332	20.167789131444497	40.10755341384115
10	13.563632141198328	29.97953437064561	34.70840106827682	21.748432419879236
11	30.453582210868557	24.42507547607989	25.81151300510915	19.309829307942405
12	19.853982814677195	27.510305387830936	30.29188922433813	22.343822573153737
13	27.0874941941477	22.241784718996747	27.9842080817464	22.68651300510915
14	20.936483975847654	23.15678704133767	27.299988388295404	28.606740594519277
15	22.8756386437529	30.405538783093355	24.470506270320485	22.248316302833256
16	23.63475383186252	29.439009521597768	23.98383070134696	22.942405945192753
17	21.29586623316303	30.736036925220624	25.528767998142126	22.43932884347422
18	21.90664189503019	27.558493961913612	27.175888295401762	23.35897584765444
19	24.238272178355782	28.27841964700418	27.235253135160242	20.248055039479794
20	22.402751973989783	26.8651300510915	27.836884579656296	22.895233395262423
21	22.302891314444963	27.99044937296795	27.14729447282861	22.559364839758477
22	20.553442870413377	30.53631560613098	26.590803529958194	22.319437993497445
23	21.40269391546679	28.704859498374365	27.41537970274036	22.477066883418487
24	23.493381328379005	29.140443567115653	27.057884347422203	20.30829075708314
25	22.65269391546679	28.414131444496054	26.951492104040874	21.981682535996285
26	23.51355666511844	28.06882837900604	27.03625754760799	21.381357408267533
27	20.926614026939156	28.853344170924295	27.080236878773807	23.13980492336275
28	20.11176265675801	28.02310729215049	29.50432535996284	22.360804691128656
29	22.439619136089178	28.07637598699489	27.36341732466326	22.120587552252672
30	20.83255921969345	29.23145030190432	27.560380863910822	22.375609614491406
31	21.638701811425918	27.471261031119372	29.16656990246168	21.723467254993032
32	22.394623780771017	28.061280771017184	27.566912447747328	21.977183000464468
33	20.703814444960518	29.145813980492335	28.72735717603344	21.4230143985137
34	20.765356479331164	29.16439270784951	28.448966558290756	21.621284254528565
35	22.51625638643753	27.50348351137947	29.102995819786344	20.877264282396656
36	22.223786576869482	28.93941593125871	25.68915466790525	23.147642823966557
37	22.422491871806784	27.378367394333488	27.79305039479796	22.406090339061773
38	22.14381096144914	26.936396888063168	28.062006502554578	22.857785647933117
39	23.156206456107757	27.25238039944264	27.517852995819787	22.07356014862982
40	21.5526300510915	26.18294240594519	30.331804458894567	21.93262308406874
41	21.638992104040874	28.042266604737577	27.178500928936366	23.140240362285184
42	22.17980724570367	27.0454017649791	28.99007199256851	21.784718996748722
43	22.173711100789596	26.20485949837436	28.92591732466326	22.695512076172783
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10.0
1	14.5
2	19.0
3	66.0
4	113.0
5	113.0
6	153.0
7	193.0
8	198.5
9	204.0
10	334.0
11	464.0
12	464.0
13	652.0
14	840.0
15	2297.0
16	3754.0
17	4146.5
18	4539.0
19	4539.0
20	5080.0
21	5621.0
22	7419.0
23	9217.0
24	11452.5
25	13688.0
26	13688.0
27	16457.5
28	19227.0
29	25182.0
30	31137.0
31	32391.0
32	33645.0
33	33645.0
34	38213.0
35	42781.0
36	43545.0
37	44309.0
38	48048.5
39	51788.0
40	51788.0
41	54599.5
42	57411.0
43	52114.5
44	46818.0
45	47784.5
46	48751.0
47	48751.0
48	48498.0
49	48245.0
50	55280.5
51	62316.0
52	55140.5
53	47965.0
54	47965.0
55	45309.0
56	42653.0
57	34849.0
58	27045.0
59	21455.0
60	15865.0
61	15865.0
62	13590.5
63	11316.0
64	9134.0
65	6952.0
66	5684.0
67	4416.0
68	4416.0
69	3629.5
70	2843.0
71	2600.5
72	2358.0
73	1946.0
74	1534.0
75	1534.0
76	1010.0
77	486.0
78	359.0
79	232.0
80	170.0
81	108.0
82	108.0
83	78.0
84	48.0
85	39.5
86	31.0
87	23.0
88	15.0
89	15.0
90	8.0
91	1.0
92	1.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	688960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.42641357747729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.53728195385551	42.27801074144548
2	8.853649200395234	8.752082540972719
3	1.99372161403009	2.9562752716022036
4	0.862334774428122	1.7048846081249978
5	0.49054394482881447	1.2122913947518092
6	0.3417359471654598	1.0134469355334568
7	0.24145146810033374	0.8353856084851313
8	0.16462504905050182	0.6509460607666071
9	0.13879838636082853	0.6174275803341115
>10	1.082161598076976	10.738666183383277
>50	0.14463424606928577	5.095309444582166
>100	0.13076115883958606	12.971482922770635
>500	0.01180687664465774	3.857116279337854
>1k	0.005608266406212426	4.267804770386196
>5k	8.855157483493304E-4	3.0488696575233556
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	7205	1.0457791453785417	No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	7094	1.0296679052484905	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	6599	0.9578204830469114	TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	3870	0.5617162099396191	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	2034	0.29522758941012545	RNA PCR Primer, Index 8 (95% over 23bp)
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1892	0.27461681374825825	No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	1888	0.2740362285183465	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1760	0.25545750116117044	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1688	0.24500696702275895	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1537	0.22308987459359036	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1486	0.21568741291221552	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1377	0.1998664653971203	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1370	0.19885044124477472	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1338	0.1942057594054807	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1225	0.17780422666047374	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1216	0.17649790989317232	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1155	0.16764398513701811	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1145	0.16619252206223875	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1104	0.1602415234556433	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1102	0.1599512308406874	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1038	0.1506618671620994	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1028	0.14921040408732003	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	958	0.13905016256386438	No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG	936	0.13585694379934973	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	918	0.13324431026474687	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	900	0.130631676730144	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	889	0.12903506734788667	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	868	0.12598699489084997	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	863	0.1252612633534603	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	767	0.11132721783557827	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	732	0.10624709707385044	No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG	716	0.10392475615420343	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.4514630747793777E-4	0.0
11	0.0	0.0	0.0	1.4514630747793777E-4	0.0
12	0.0	0.0	0.0	2.9029261495587555E-4	0.0
13	0.0	0.0	0.0	2.9029261495587555E-4	0.0
14	0.0	0.0	0.0	4.3543892243381327E-4	0.0
15	0.0	0.0	0.0	4.3543892243381327E-4	0.0
16	0.0	0.0	0.0	5.805852299117511E-4	0.0
17	0.0	0.0	0.0	5.805852299117511E-4	0.0
18	0.0	0.0	0.0	5.805852299117511E-4	0.0
19	0.0	0.0	0.0	5.805852299117511E-4	0.0
20	0.0	0.0	0.0	7.257315373896888E-4	0.0
21	0.0	0.0	0.0	0.00130631676730144	0.0
22	0.0	0.0	0.0	0.0015966093822573153	0.0
23	0.0	0.0	0.0	0.0015966093822573153	0.0
24	0.0	0.0	0.0	0.0020320483046911285	0.0
25	0.0	0.0	0.0	0.0020320483046911285	0.0
26	0.0	0.0	0.0	0.0023223409196470044	0.0
27	0.0	0.0	0.0	0.0027577798420808175	0.0
28	0.0	0.0	0.0	0.0058058522991175105	0.0
29	0.0	0.0	0.0	0.01103111936832327	0.0
30	0.0	0.0	0.0	0.02148165350673479	0.0
31	0.0	0.0	0.0	0.05428471899674872	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	935	0.0	32.844917	1
TTATACA	1055	0.0	29.985783	2
CGTCTTC	280	0.0	28.410715	37
TATACAC	1140	0.0	27.91228	3
CCGTCTT	940	0.0	26.569149	37
GCCGTCT	985	0.0	25.355331	36
GGTATCA	325	0.0	25.046156	1
GTATCAA	975	0.0	24.85641	1
ACGGTAC	45	1.3224603E-4	24.666668	3
CACGGTA	50	2.7005796E-4	22.2	2
CGGTACT	50	2.7005796E-4	22.2	4
TGCCGTC	1165	0.0	21.437769	35
GACGTAG	45	0.003824275	20.555557	25
ATGCCGT	1270	0.0	19.665354	34
AGACGTA	50	0.007032091	18.5	24
TCTCGTG	50	0.007032091	18.5	24
ACCGTGC	90	2.149669E-6	18.5	9
TATGCCG	1380	0.0	18.097826	33
AACGGCC	75	2.0657519E-4	17.266666	37
TCACGGT	65	0.0015790658	17.076923	1
>>END_MODULE