FastQCFastQC Report
Fri 10 Feb 2017
ERR1631215.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631215.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932896
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40560.43477515178540804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40080.42962988371694166No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT39490.42330549171611837No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG35220.3775340445237197No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT32240.3455905052653243TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC21920.23496724179329745No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA18540.19873597914451344No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC15010.1608968202243337No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT13290.14245960964566254No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC13110.14053013411998766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12980.13913662401811136No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC12870.13795750008575447No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC12570.134741707542963No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA12210.1308827564916132No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA11980.12841731554213975No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT10910.11694765547285015No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC10670.11437502143861694RNA PCR Primer, Index 6 (95% over 22bp)
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG10420.11169519431962405No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCT10250.10987291187870887No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA10030.10751466401399513No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC9840.10547799540356054No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT9350.10022553425033444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTCG308.310308E-637.07
GGTATCA4750.028.431581
GTATCAA18200.027.038461
CTTATAC5450.026.8165131
CGTCTTC1800.026.72222337
AGTTCGC451.3229508E-424.6666688
CCGTTTA451.3229508E-424.66666827
TATACAC6500.023.6230773
GCCGTCT5400.023.29629736
CCGTCTT5500.022.87272837
TTGGCGT502.7015732E-422.223
GACCGTT502.7015732E-422.27
AGGACCG502.7015732E-422.25
TTACCGG502.7015732E-422.231
GATTACG603.725318E-521.5833321
TTATACA7050.021.517732
GGACCGT705.1002244E-621.1428576
GGTTCGC1151.3460522E-1020.91304423
CGCCGTT555.1420956E-420.18181825
TTACGGG759.262705E-619.7333343
Produced by FastQC (version 0.11.5)