##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631215.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	932896
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.531913525194664	31.0	31.0	34.0	28.0	34.0
2	31.5254508541145	31.0	31.0	34.0	30.0	34.0
3	30.993887850307	31.0	31.0	34.0	27.0	34.0
4	35.1384495163448	37.0	35.0	37.0	32.0	37.0
5	35.352665248859466	37.0	35.0	37.0	33.0	37.0
6	35.55039039721469	37.0	35.0	37.0	33.0	37.0
7	35.560749536925876	37.0	35.0	37.0	33.0	37.0
8	35.58476936335883	37.0	35.0	37.0	33.0	37.0
9	37.345241055809005	39.0	37.0	39.0	34.0	39.0
10	37.19488881933249	39.0	37.0	39.0	33.0	39.0
11	37.258796264535384	39.0	37.0	39.0	34.0	39.0
12	37.2032466641512	39.0	37.0	39.0	33.0	39.0
13	37.26012224299386	39.0	37.0	39.0	34.0	39.0
14	38.42303107741913	40.0	38.0	41.0	34.0	41.0
15	38.44172233560869	40.0	38.0	41.0	34.0	41.0
16	38.44323268617295	40.0	38.0	41.0	34.0	41.0
17	38.43989147772099	40.0	38.0	41.0	34.0	41.0
18	38.46232163070696	40.0	38.0	41.0	34.0	41.0
19	38.51465329468665	40.0	38.0	41.0	34.0	41.0
20	38.4965633897026	40.0	38.0	41.0	34.0	41.0
21	38.45828581106576	40.0	38.0	41.0	34.0	41.0
22	38.42526069358214	40.0	38.0	41.0	34.0	41.0
23	38.39339111755223	40.0	38.0	41.0	34.0	41.0
24	38.36964141769286	40.0	38.0	41.0	34.0	41.0
25	38.35751895173738	40.0	38.0	41.0	34.0	41.0
26	38.2548847888725	40.0	38.0	41.0	34.0	41.0
27	38.20287577607793	40.0	38.0	41.0	34.0	41.0
28	38.16398934071965	40.0	38.0	41.0	33.0	41.0
29	38.105456556786606	40.0	38.0	41.0	33.0	41.0
30	38.02404448084245	40.0	37.0	41.0	33.0	41.0
31	37.969938771309984	40.0	37.0	41.0	33.0	41.0
32	37.919131392995574	40.0	37.0	41.0	33.0	41.0
33	37.887540518986036	40.0	37.0	41.0	33.0	41.0
34	37.82542534216033	40.0	37.0	41.0	33.0	41.0
35	37.79168739066306	40.0	37.0	41.0	33.0	41.0
36	37.74352339381882	40.0	37.0	41.0	33.0	41.0
37	37.711322591157	40.0	37.0	41.0	33.0	41.0
38	37.65199229067334	40.0	37.0	41.0	33.0	41.0
39	37.58052451720235	40.0	37.0	41.0	32.0	41.0
40	37.53668468939732	40.0	37.0	41.0	32.0	41.0
41	37.47336144650636	40.0	37.0	41.0	32.0	41.0
42	37.42129562137687	40.0	37.0	41.0	32.0	41.0
43	36.846166132130485	39.0	36.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	5.0
11	3.0
12	4.0
13	6.0
14	4.0
15	1.0
16	4.0
17	10.0
18	19.0
19	27.0
20	67.0
21	140.0
22	284.0
23	483.0
24	960.0
25	1699.0
26	2965.0
27	4475.0
28	6785.0
29	9841.0
30	13877.0
31	18580.0
32	24836.0
33	32621.0
34	43881.0
35	58807.0
36	83288.0
37	125161.0
38	221647.0
39	282409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.82147634891778	23.161209824031832	15.652977395122287	22.364336431928102
2	13.528196069015197	23.865575583987926	41.19773265187116	21.408495695125715
3	14.687060508352484	28.411527115562723	35.74256937536446	21.15884300072034
4	12.022133228141186	18.859015367200634	40.90016464857819	28.21868675607999
5	11.821360580386237	39.71160772476246	36.21518368607005	12.251848008781257
6	26.31150739203513	42.00393184234898	18.563269646348573	13.121291119267314
7	23.24063989983878	34.822102356532774	24.5851627619799	17.352094981648545
8	23.88669262168559	36.612441258189556	22.168709052241624	17.332157067883237
9	24.47486107776215	16.267944122388776	21.50518385757898	37.75201094227009
10	13.945069975645733	29.78520649675848	35.16790724796762	21.101816279628167
11	30.816082392892667	24.87908620039104	26.372821836517684	17.932009570198606
12	20.157873975234107	27.677147291873904	31.874935684149143	20.290043048742838
13	26.9081441018077	22.830733543717628	28.527617226357492	21.733505128117176
14	20.639278118889994	23.104719068363462	29.517438171028708	26.738564641717833
15	22.307952869344494	30.383022330463415	25.775649161321308	21.533375638870787
16	21.6863401708229	30.17571090453813	25.916608067780334	22.221340856858642
17	20.743898569615478	29.8841457139917	27.773299488903376	21.598656227489453
18	21.525014578259526	28.299188762734538	28.930556031969267	21.24524062703667
19	23.02250197235276	27.798275477652385	29.372513120433574	19.80670942956128
20	23.16131701711659	27.334343806812335	29.028423318354886	20.47591585771619
21	21.608625184372105	28.581428154906867	28.4272844990224	21.382662161698622
22	20.517292388433436	29.550132061880426	29.137009913216477	20.79556563646966
23	20.97790107364594	28.256740301169692	29.057151065070492	21.70820756011388
24	21.895795458443384	28.57381744588893	28.682725620004803	20.847661475662882
25	22.227558055774708	27.974608102082115	28.914369876170547	20.883463965972627
26	21.329387198573045	28.348497581724008	28.761083764964155	21.561031454738792
27	21.318453503927554	28.922516550612286	28.74479041608068	21.01423952937948
28	20.302370253490206	28.99004819401091	29.406707714471924	21.300873838026963
29	21.394453401022194	28.75240112509862	28.71788495180599	21.1352605220732
30	21.336890714506225	28.93484375535965	28.810499777038384	20.917765753095736
31	21.45683977635235	28.873529310877093	28.870742290673345	20.798888622097213
32	21.2190855143553	28.26188556923816	29.53480345075978	20.984225465646762
33	20.893861695194317	27.885852227901076	30.240884300072036	20.979401776832574
34	20.846267965561005	29.035712448118545	28.428678009124276	21.68934157719617
35	21.232163070695982	28.633416801015333	29.59708263300518	20.537337495283502
36	21.548168284567627	28.51346773916921	28.307335437176278	21.631028539086884
37	22.102678112029636	27.413452303365005	28.80256748876616	21.681302095839193
38	21.749369704661614	27.92883665488972	29.00719908757246	21.314594552876205
39	21.672083490549856	27.881350118341164	29.131328679724213	21.315237711384764
40	21.16109405550029	27.461903577676395	30.028106026824	21.348896339999314
41	21.166453709738278	27.950168078756903	29.05168421774774	21.831693993757074
42	21.51954773093678	27.404233698075668	29.42493053888108	21.65128803210647
43	21.022600589990738	27.26884883202415	29.811575995609374	21.89697458237574
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	39.5
2	44.0
3	157.5
4	271.0
5	271.0
6	328.5
7	386.0
8	403.0
9	420.0
10	653.5
11	887.0
12	887.0
13	1381.5
14	1876.0
15	4839.0
16	7802.0
17	8780.0
18	9758.0
19	9758.0
20	10585.0
21	11412.0
22	14670.5
23	17929.0
24	22197.0
25	26465.0
26	26465.0
27	30445.5
28	34426.0
29	41504.5
30	48583.0
31	51052.5
32	53522.0
33	53522.0
34	55621.0
35	57720.0
36	60783.0
37	63846.0
38	65764.0
39	67682.0
40	67682.0
41	70733.5
42	73785.0
43	68416.0
44	63047.0
45	63149.0
46	63251.0
47	63251.0
48	61283.5
49	59316.0
50	62436.5
51	65557.0
52	61949.0
53	58341.0
54	58341.0
55	53015.0
56	47689.0
57	41549.0
58	35409.0
59	28789.5
60	22170.0
61	22170.0
62	18911.0
63	15652.0
64	13087.5
65	10523.0
66	8552.0
67	6581.0
68	6581.0
69	5147.0
70	3713.0
71	3048.5
72	2384.0
73	1845.5
74	1307.0
75	1307.0
76	950.5
77	594.0
78	442.5
79	291.0
80	204.0
81	117.0
82	117.0
83	95.0
84	73.0
85	47.5
86	22.0
87	14.5
88	7.0
89	7.0
90	4.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	932896.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.81786798084022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.24385201733483	33.72269026082791
2	14.117911914050099	12.654694246580572
3	4.476112344772313	6.018294364062441
4	1.9626702743248656	3.5185078897844515
5	0.9849249806681807	2.207111887730907
6	0.6092648697764446	1.6383571499402703
7	0.40009943568609313	1.2552122581451597
8	0.2698106987218034	0.9673872220105634
9	0.21806856008981246	0.8796031143189423
>10	1.3888248528404965	12.069692736853735
>50	0.16927301395134553	5.331168090060577
>100	0.1459814765828079	13.14713941183118
>500	0.008403538946014977	2.454220211237864
>1k	0.004802022254865701	4.135921156615395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4056	0.43477515178540804	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4008	0.42962988371694166	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	3949	0.42330549171611837	No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG	3522	0.3775340445237197	No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	3224	0.3455905052653243	TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	2192	0.23496724179329745	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1854	0.19873597914451344	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1501	0.1608968202243337	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1329	0.14245960964566254	No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	1311	0.14053013411998766	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1298	0.13913662401811136	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1287	0.13795750008575447	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1257	0.134741707542963	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1221	0.1308827564916132	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1198	0.12841731554213975	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1091	0.11694765547285015	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	1067	0.11437502143861694	RNA PCR Primer, Index 6 (95% over 22bp)
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1042	0.11169519431962405	No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCT	1025	0.10987291187870887	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1003	0.10751466401399513	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	984	0.10547799540356054	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	935	0.10022553425033444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.0719308475971598E-4	0.0	0.0	1.0719308475971598E-4	0.0
9	1.0719308475971598E-4	0.0	0.0	1.0719308475971598E-4	0.0
10	1.0719308475971598E-4	0.0	0.0	1.0719308475971598E-4	0.0
11	1.0719308475971598E-4	0.0	0.0	1.0719308475971598E-4	0.0
12	1.0719308475971598E-4	0.0	0.0	2.1438616951943197E-4	0.0
13	1.0719308475971598E-4	0.0	0.0	2.1438616951943197E-4	0.0
14	1.0719308475971598E-4	0.0	0.0	2.1438616951943197E-4	0.0
15	1.0719308475971598E-4	0.0	0.0	2.1438616951943197E-4	0.0
16	1.0719308475971598E-4	0.0	0.0	3.2157925427914793E-4	0.0
17	1.0719308475971598E-4	0.0	0.0	3.2157925427914793E-4	0.0
18	1.0719308475971598E-4	0.0	0.0	3.2157925427914793E-4	0.0
19	1.0719308475971598E-4	0.0	0.0	4.2877233903886394E-4	0.0
20	2.1438616951943197E-4	0.0	0.0	5.359654237985799E-4	0.0
21	4.2877233903886394E-4	0.0	0.0	7.503515933180119E-4	0.0
22	5.359654237985799E-4	0.0	0.0	0.0012863170171165917	0.0
23	5.359654237985799E-4	0.0	0.0	0.0012863170171165917	0.0
24	5.359654237985799E-4	0.0	0.0	0.0016078962713957398	0.0
25	5.359654237985799E-4	0.0	0.0	0.0017150893561554558	0.0
26	5.359654237985799E-4	0.0	0.0	0.0019294755256748877	0.0
27	5.359654237985799E-4	0.0	0.0	0.0031085994580317636	0.0
28	5.359654237985799E-4	0.0	0.0	0.011576853154049325	0.0
29	5.359654237985799E-4	0.0	0.0	0.025190374918533254	0.0
30	5.359654237985799E-4	0.0	0.0	0.04330600624292526	0.0
31	5.359654237985799E-4	0.0	0.0	0.09829605872465956	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTCG	30	8.310308E-6	37.0	7
GGTATCA	475	0.0	28.43158	1
GTATCAA	1820	0.0	27.03846	1
CTTATAC	545	0.0	26.816513	1
CGTCTTC	180	0.0	26.722223	37
AGTTCGC	45	1.3229508E-4	24.666668	8
CCGTTTA	45	1.3229508E-4	24.666668	27
TATACAC	650	0.0	23.623077	3
GCCGTCT	540	0.0	23.296297	36
CCGTCTT	550	0.0	22.872728	37
TTGGCGT	50	2.7015732E-4	22.2	23
GACCGTT	50	2.7015732E-4	22.2	7
AGGACCG	50	2.7015732E-4	22.2	5
TTACCGG	50	2.7015732E-4	22.2	31
GATTACG	60	3.725318E-5	21.583332	1
TTATACA	705	0.0	21.51773	2
GGACCGT	70	5.1002244E-6	21.142857	6
GGTTCGC	115	1.3460522E-10	20.913044	23
CGCCGTT	55	5.1420956E-4	20.181818	25
TTACGGG	75	9.262705E-6	19.733334	3
>>END_MODULE