FastQCFastQC Report
Fri 10 Feb 2017
ERR1631214.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631214.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322303
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT147274.56930279891903No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG138684.302783405677267No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT117203.6363297890494346Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC78402.432493647282216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55911.7347030589228147No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51131.586395410529843No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC41531.2885390455565104Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC40511.2568918067780939No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA27720.8600602538604977No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG18990.5891971219628734No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18980.5888868549160262No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA18580.5764761730421374No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA18500.5739940366673596No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT15900.49332460448708204No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA12800.39714181996444337No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT12570.3900056778869573No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12440.38597220627794343No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT11850.36766645051395735No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11800.36611511527972124No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11630.3608405754833185No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10850.3366397458292352No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT9850.3056130411445131No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG9840.3053027740976659No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8170.2534881772741799No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8060.2500752397588605No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7990.24790337043092991No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7830.24293909768137437No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7830.24293909768137437No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7430.2305284158074855No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7350.22804627943270772No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7270.22556414305792996No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7160.22215120554261053No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6860.21284319413719388No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6760.20974052366872167No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6590.2044659838723189No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6520.20229411454438834No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6470.20074277931015225No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6430.19950171112276335No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6300.19546823951374948No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6250.19391690427951336No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6180.19174503495158282No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5910.18336782468670784No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5690.17654194965606898No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5650.17530088146868011No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5310.16475180187587457No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5160.16009779617316625No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5130.15916699503262458No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5130.15916699503262458No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG5040.1563745916109996No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5010.15544379047045792No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4860.1507897847677496No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4850.1504795177209024No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4710.1461357790650413No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.1389996369875552No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4450.13806883584701352No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4440.1377585688001663No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4410.13682776765962465No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4320.13403536423799964No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4250.1318634949100691No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4200.130312159675833No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4190.1300018926289858No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4170.1293813585352913No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4170.1293813585352913No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4070.1262786880668191No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4040.12534788692627746No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3960.12286575055149968No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3950.12255548350465245No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3800.11790147780194413No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3800.11790147780194413No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3780.11728094370824968No Hit
ATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT3770.11697067666140246No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3750.11635014256770804No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3750.11635014256770804No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3690.11448854028662468No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3690.11448854028662468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3630.11262693800554137No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3610.11200640391184694No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3510.10890373344337471No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT3310.10269839250643029No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3300.10238812545958306No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3300.10238812545958306No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3280.10176759136588862No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA3280.10176759136588862No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3270.10145732431904139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG501.7826096E-1037.02
TAGCAAC308.293426E-637.01
TCAGAGT251.2316552E-437.03
TTACGGG452.6084308E-937.03
GATTACG501.7826096E-1037.01
ACCTTGA200.001839971837.09
CCTTGAA200.001839971837.010
CTTATAC17000.035.802941
ATTACTT507.2977855E-933.3000032
TTATACA18350.033.2697532
CGTCTTC4900.032.09183537
TATACAC19200.031.7968733
AGCAACG352.3830169E-531.7142872
CTCAGAG303.593784E-430.8333322
CAGACAC601.2641976E-930.83333218
TTACTTT551.8470018E-830.2727283
GTGTATA551.8470018E-830.2727286
TAAGAGA551.8470018E-830.27272811
TTTACAC250.005490548429.62
AAAAGTA250.005490548429.615
Produced by FastQC (version 0.11.5)